- 询价
- R&D
- ZK-0002370
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- GFAP (SMI 21) Monoclonal Antibody保存于运输说明：
- Store at -20°C.
- 详细信息：
-
Description Monoclonal Antibody against Glial Fibrillary Acidic Protein (GFAP) Clone SMI 21 Form Ascites Fluid (Stored in 0.01 M sodium azide) Host Mouse Species Reactivity Human, monkey, canine IsoType GFAP (SMI 21) Monoclonal AntibodyIgG1 Specificity SMI 21 has been tested for immunocytochemical localization of GFAP in astrocytes and Bergmann glia in human, monkey and dog paraffin sections. Astrocytomas from human and dog show a positive reaction with SMI 21. Rat, rabbit and mouse brain sections do not react with SMI 21. Other species have not been examined. No reactivity was observed on liver and kidney paraffin sections. SMI 21 reacts with GFAP and shows no cross reactivity with other intermediate filaments on tissue sections or Western blots of human brain proteins. Rat cytoskeletal proteins do not react with SMI 21. Uses This antibody is effective in immunoblotting, immunohistochemistry and ELISA. Suggested Working Dilution The extent of dilution of SMI 21 beyond those recommended above depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined. This antibody is sold for research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- IHC: 1:1,000
- GFAP (SMI 21) Monoclonal AntibodyELISA: 1:1,000
Tissue Preparation: SMI 21 can be used to detect GFAP in paraffin, vibratome and frozen tissue sections and cell cultures. In order to obtain antibody reactivity on paraffin sections, the sections (on glue-coated slides) can be incubated with 0.1% trypsin in 0.05M Tris buffer pH 7.6 for 20 to 30 minutes at 37°C after paraffin removal. Glue-coated slides are prepared by mixing 2.25g of Elmers glue in 15 mL distilled water. Apply one drop of glue solution to a clean glass slide and smear over the slide with another clean slide. Dry overnight in 56°C oven. Cut paraffin sections, place on glue covered slide and dry. Enhanced reaction of GFAP in paraffin sections also has been reported with tissue fixed in FEA fixative (formalin, ethanol, acetic acid and water; 4:69:5:22(v/v) and after autoclaving of deparaffinized formalin fixed sections in distilled water or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min. Post-fixation for 10 minutes in cold methanol or methanol/hydrogen peroxide is necessary for good access of SMI 22 to astrocytes in culture or in cryostat and vibratome tissue sections.Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - GFAP (SMI 21) Monoclonal Antibody
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GFAP (SMI 21) Monoclonal Antibody
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