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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- Keratin 10 Polyclonal Antibody, Purified详细信息:
-
Description 
Polyclonal Antibody against Keratin 10 Intended Use 
** In Vitro Diagnostic (IVD) ** Form 
Affinity Purified Antibody (Stored in PBS + 0.03% Thimerosal) Host 
Rabbit Species Reactivity 
Mouse, Human [Ab] 
1 mg/mL Specificity 
Keratin 10 Polyclonal Antibody, Purified This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 10 protein. It was subsequently purified using peptide affinity chromatography. Uses 
This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. - WB: 1:1,000*
- IF: 1:500
- IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250) or polymer based detection systems such as ACUITYAdvanced Biotin Free Polymer Detection (SIG-32902).
Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (SIG-31932, SIG-31934).
Incubation: 60 minutes at room temperature
Notes 
*Predicted MW = 59 kDa Storage 
When stored at ≤20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first. References 
Keratin 10 Polyclonal Antibody, Purified Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001. [IF]
Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989. [WB, IF]
Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - Keratin 10 Polyclonal Antibody, Purified
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