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- R&D
- 美国/中国
- xy-102P
- 2025年07月12日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- NUP 153 Nuclear Pore Complex Protein Monoclonal Antibody保存于运输说明：
- Store at -20°C.
- 详细信息：
-
Description 
Monoclonal Antibody against Nuclear Pore Complex Proteins, NUP 153 Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone QE5 Form Purified Antibody (Stored in PBS + 0.03% Thimerosal) Host Mouse Species Reactivity NUP 153 Nuclear Pore Complex Protein Monoclonal Antibody Eukaryote IsoType IgG1 [Ab] 1 mg/mL Specificity The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins. It recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern. Uses This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:500
- IF: 1:250
- IP: 1:50
Storage NUP 153 Nuclear Pore Complex Protein Monoclonal Antibody Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References C Smythe, HE Jenkins, CJ Hutchison. Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs. EMBO J 19(15):3918-3931, 2000.
N Panté, R Bastos, I McMorrow, B Burke, U Aebi. Interactions and three dimensional localization of a group of nuclear pore complex proteins. J Cell Biol 126:603-617, 1994.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - NUP 153 Nuclear Pore Complex Protein Monoclonal Antibody
- xy-0295G-xyy3 Goat Anti-rabbit IgG/Cy3Cy3标记的羊抗兔IgG
xy-0295G-xyy5.5 Goat Anti-rabbit IgG/Cy5.5Cy5.5标记的羊抗兔IgG
xy-0295G-AF350 Goat Anti-rabbit IgG/Alexa Fluor 350Alexa Fluor 350标记的羊抗兔IgG
xy-0295G-AF488 Goat Anti-rabbit IgG/Alexa Fluor 488Alexa Fluor 488标记的羊抗兔IgG
xy-0296G-AF350 Goat Anti-Mouse IgG/Alexa Fluor 350Alexa Fluor 350标记的羊抗小鼠IgG
xy-0296G-AF488 Goat Anti-Mouse IgG/Alexa Fluor 488Alexa Fluor 488标记的羊抗小鼠IgG
xy-0296G-AF555 Goat Anti-Mouse IgG/Alexa Fluor 555Alexa Fluor 555标记的羊抗小鼠IgG
xy-0296G-AF647 Goat Anti-Mouse IgG/Alexa Fluor 647Alexa Fluor 647标记的羊抗小鼠IgG
xy-0296G-xyE-xyY5 Goat Anti-Mouse IgG/PE-CY5PE-CY5标记的羊抗小鼠IgG
xy-0296G-xyE-xyy7 Goat Anti-Mouse IgG/PE-Cy7PE-Cy7标记的羊抗小鼠IgG
xy-0296G-xyy3 Goat Anti-Mouse IgG/Cy3Cy3标记的羊抗小鼠IgG
xy-0296G-xyE Goat Anti-Mouse IgG/PEPE标记的羊抗小鼠IgG
xy-0358M-xyE-xyy7 Mouse Anti-Guinea pig IgG/PE-Cy7PE-Cy7标记的小鼠抗豚鼠IgG
xy-0358G-xyy3 Goat Anti-Guinea pig IgG/Cy3Cy3标记的羊抗豚鼠IgG
xy-0295G-HRxy Goat Anti-rabbit IgG/HRP辣根过氧化物酶标记的羊抗兔IgG
xy-0294R-xyE Rabbit Anti-Goat IgG/PEPE标记的兔抗羊IgG
xy-0294xy-AF647 Donkey Anti-Goat IgG/Alexa Fluor 647Alexa Fluor 647标记的驴抗羊IgG
xy-0294xy-xyE-xyy5.5 Donkey Anti-Goat IgG/PE-Cy5.5PE-Cy5.5标记的驴抗羊IgG
xy-0294M-AF350 Mouse Anti-Goat IgG/Alexa Fluor 350Alexa Fluor 350标记的小鼠抗羊IgG
xy-0294M-AF555 Mouse Anti-Goat IgG/Alexa Fluor 555Alexa Fluor 555标记的小鼠抗羊IgG
xy-0375R-xyE-xyy3 Rabbit Anti-Guinea pig IgM/PE-Cy3PE-Cy3标记的兔抗豚鼠IgM
xy-0296G-Golxy Goat Anti-Mouse IgG/Gold胶体金标记的羊抗小鼠IgG
xy-0295G-Golxy Goat Anti-rabbit IgG/Gold胶体金标记的羊抗兔IgG
xy-0296G-RBITxy Goat Anti-Mouse IgG/RBITC罗丹明标记的羊抗小鼠IgG
xy-0295G-RBITxy Goat Anti-rabbit IgG/RBITC罗丹明标记的羊抗兔IgG
xy-0294xy-FITxy Donkey Anti-Goat IgG/FITCFITC标记的驴抗羊IgG
xy-0294M-FITxy Mouse Anti-Goat IgG/FITCFITC标记的小鼠抗羊IgG
xy-0296G-HRxy Goat Anti-Mouse IgG/HRP辣根过氧化物酶标记的羊抗小鼠IgG
xy-0294R-HRxy Rabbit Anti-Goat IgG/HRP辣根过氧化物酶标记的兔抗羊IgG
xy-0370R-AF555 Rabbit Anti-Goat IgM/Alexa Fluor 555Alexa Fluor 555标记的兔抗羊IgM
xy-0370R-xyE-xyy5.5 Rabbit Anti-Goat IgM/PE-Cy5.5PE-Cy5.5标记的兔抗羊IgM
xy-0370R-xyE-xyy7 Rabbit Anti-Goat IgM/PE-Cy7PE-Cy7标记的兔抗羊IgM
xy-0358xy-xyE-xyy7 Donkey Anti-Guinea pig IgG/PE-Cy7PE-Cy7标记的驴抗豚鼠IgG
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文献和实验Monoclonal Antibody Affinity Chromatography
. These criteria are often best fulfilled for protein targets by antibody ligands. Monoclonal antibodies are the ligands of choice for most purposes, especially when a protein of unknown properties is being characterized. Monoclonal antibodies fulfill
Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
proteins. Taking advantage of the exquisite specificity afforded by each antibody results in a final purified product that binds specifically to that MAb. Using immunoaffinity chromatography, a single antigen can be separated from very complex mixtures (e.g
施一公团队突破性进展再登 Science！以超高分辨率解析核孔复合物的结构细节
质组装成正确的三维分子结构一直是一个巨大的挑战。 2022 年 6 月 10 日，西湖大学施一公团队在 Science 杂志发表研究论文 Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex。 该研究聚焦于非洲爪蟾核孔复合物（NPC）结构，首次扩展了脊椎动物细胞质环（CR）亚单位复合体模型的分子量，获得高分辨率的单粒子冷冻电镜显微结构。除了解析 Y 复合物，同时发现 5 个核孔蛋白 Nup358
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