- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA771Hu
- 2025年07月01日
13 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
趋化因子C-X-C-基元配体16(CXCL16)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.057ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
趋化因子C-X-C-基元配体16(CXCL16)检测试剂盒
yb-E10242 人前心钠肽(Pro-ANP)ELISA试剂盒 Human atrial natriuretic peptide before (Pro-ANP) ELISA kit
yb-E10243 人细胞角蛋白13(CK-13)ELISA试剂盒 Human cytokeratin 13 (CK-13) ELISA kit
yb-E10244 人细胞角蛋白17(CK17)ELISA试剂盒 Human cytokeratin 17 (CK17) ELISA kit
yb-E10245 人制瘤素M受体(OSMR)ELISA试剂盒 Human oncostatin M receptor (OSMR) ELISA kit
yb-E10246 人B细胞淋巴瘤因子3(Bcl3)ELISA试剂盒 Human B -cell lymphoma factor 3 (Bcl3) ELISA kit
yb-E10247 人癌蛋白诱导转录物3(OIT3)ELISA试剂盒 Human oncoprotein induced transcript 3 (OIT3) ELISA kit
yb-E10248 人P27蛋白(P27)ELISA试剂盒 Human P27 protein (P27) ELISA kit
yb-E10249 人P糖蛋白/渗透性糖蛋白(P-gp)ELISA试剂盒 Human P-glycoprotein / permeability glycoprotein (P-gp) ELISA kit
yb-E10250 人大肠癌专一抗原3(CCSA-3)ELISA试剂盒 Colorectal cancer -specific antigen 3 (CCSA-3) ELISA kit
yb-E10251 人大肠癌专一抗原2(CCSA-2)ELISA试剂盒 Colorectal cancer -specific antigen 2 (CCSA-2) ELISA Kit
yb-E10252 人大肠癌专一抗原4(CCSA-4)ELISA试剂盒 Colorectal cancer -specific antigen 4 (CCSA-4) ELISA kit
yb-E10253 人粘蛋白/粘液素5B(MUC5B)ELISA试剂盒 Human mucin / mucin- 5B (MUC5B) ELISA Kit
yb-E10254 人肠三叶因子(ITF)ELISA试剂盒 Human intestinal trefoil factor (ITF) ELISA kit
yb-E10255 人Dickkopf 1(DKK1)ELISA试剂盒 Human Dickkopf 1 (DKK1) ELISA Kit
yb-E10256 人激肽释放酶11(KLK 11)ELISA试剂盒 Human kallikrein 11 (KLK 11) ELISA kit
yb-E10257 人生长调节致癌基因γ/黑素瘤生激因子(GROγ/CXCL3/MGSA)ELISA试剂盒 Human growth regulating oncogene γ / melanoma growth stimulating factor (GROγ/CXCL3/MGSA) ELISA Kit
yb-E10258 人生长调节致癌基因β/黑素瘤生激因子(GROβ/CXCL2/MGSA)ELISA试剂盒 Oncogenic human growth regulating β / melanoma growth stimulating factor (GROβ/CXCL2/MGSA) ELISA Kit
yb-E10259 人美丽线虫凋亡基因(CED-3)ELISA试剂盒 Beautiful people nematode apoptotic genes (CED-3) ELISA kit
yb-E10260 人胸腺白血病抗原(TLa)ELISA试剂盒 Human thymus leukemia antigen (TLa) ELISA kit
yb-E10261 人肿瘤特异性移植抗原(TSTA)ELISA试剂盒 Human tumor specific transplantation antigen (TSTA) ELISA Kit
yb-E10262 人足细胞标记蛋白/足盂蛋白(PCX)ELISA试剂盒 Human podocyte marker protein / protein spoon foot (PCX) ELISA Kit
yb-E10263 人乳腺癌易感蛋白1(BRCA-1)ELISA试剂盒 Human breast cancer susceptibility protein 1 (BRCA-1) ELISA Kit
yb-E10264 人T细胞急性淋巴母细胞白血病相关抗原(TALLA-1/CD231)ELISA试剂盒 Human T -cell acute lymphoblastic leukemia -associated antigen (TALLA-1/CD231) ELISA kit
yb-E10265 人核仁形成区嗜银蛋白(Ag-NORs)ELISA试剂盒 People nucleolar organizer regions associated proteins (Ag-NORs) ELISA kit
yb-E10266 人硫氧化还原蛋白(Trx)ELISA试剂盒 Human thioredoxin (Trx) ELISA kit
yb-E10267 人窖蛋白(Cav-1)ELISA试剂盒 Human caveolin (Cav-1) ELISA Kit
yb-E10268 人普通急性淋巴细胞白血病抗原(CALLA)ELISA试剂盒 Ordinary acute lymphoblastic leukemia antigen (CALLA) ELISA kit
yb-E10269 人黑色素细胞刺激素(MSH)ELISA试剂盒 Human melanocyte stimulating hormone (MSH) ELISA kit
yb-E10270 人表皮角蛋白(EK)ELISA试剂盒 Human epidermal keratin (EK) ELISA Kit
趋化因子C-X-C-基元配体16(CXCL16)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.057ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
趋化因子C-X-C-基元配体16(CXCL16)检测试剂盒
ELISA Kit for Chemokine C-X-C-Motif Ligand 16 (CXCL16)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA771Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.062ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Chemokine C-X-C-Motif Ligand 16 (CXCL16).
No significant cross-reactivity or interference between Chemokine C-X-C-Motif Ligand 16 (CXCL16) and analogues was observed.
No significant cross-reactivity or interference between Chemokine C-X-C-Motif Ligand 16 (CXCL16) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Chemokine C-X-C-Motif Ligand 16 (CXCL16) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-X-C-Motif Ligand 16 (CXCL16) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 91-99 | 94 |
| heparin plasma(n=5) | 96-103 | 101 |
Precision
趋化因子C-X-C-基元配体16(CXCL16)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X-C-Motif Ligand 16 (CXCL16) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X-C-Motif Ligand 16 (CXCL16) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X-C-Motif Ligand 16 (CXCL16) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-X-C-Motif Ligand 16 (CXCL16) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-97% | 85-104% | 82-97% | 98-105% |
| EDTA plasma(n=5) | 84-93% | 98-105% | 89-98% | 89-101% |
| heparin plasma(n=5) | 79-98% | 87-96% | 80-94% | 81-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
趋化因子C-X-C-基元配体16(CXCL16)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Ligand 16 (CXCL16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Ligand 16 (CXCL16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Ligand 16 (CXCL16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Ligand 16 (CXCL16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10241 人N端前脑钠素(NT-proBNP)ELISA试剂盒 Human N -terminal pro- brain natriuretic peptide (NT-proBNP) ELISA kit
yb-E10242 人前心钠肽(Pro-ANP)ELISA试剂盒 Human atrial natriuretic peptide before (Pro-ANP) ELISA kit
yb-E10243 人细胞角蛋白13(CK-13)ELISA试剂盒 Human cytokeratin 13 (CK-13) ELISA kit
yb-E10244 人细胞角蛋白17(CK17)ELISA试剂盒 Human cytokeratin 17 (CK17) ELISA kit
yb-E10245 人制瘤素M受体(OSMR)ELISA试剂盒 Human oncostatin M receptor (OSMR) ELISA kit
yb-E10246 人B细胞淋巴瘤因子3(Bcl3)ELISA试剂盒 Human B -cell lymphoma factor 3 (Bcl3) ELISA kit
yb-E10247 人癌蛋白诱导转录物3(OIT3)ELISA试剂盒 Human oncoprotein induced transcript 3 (OIT3) ELISA kit
yb-E10248 人P27蛋白(P27)ELISA试剂盒 Human P27 protein (P27) ELISA kit
yb-E10249 人P糖蛋白/渗透性糖蛋白(P-gp)ELISA试剂盒 Human P-glycoprotein / permeability glycoprotein (P-gp) ELISA kit
yb-E10250 人大肠癌专一抗原3(CCSA-3)ELISA试剂盒 Colorectal cancer -specific antigen 3 (CCSA-3) ELISA kit
yb-E10251 人大肠癌专一抗原2(CCSA-2)ELISA试剂盒 Colorectal cancer -specific antigen 2 (CCSA-2) ELISA Kit
yb-E10252 人大肠癌专一抗原4(CCSA-4)ELISA试剂盒 Colorectal cancer -specific antigen 4 (CCSA-4) ELISA kit
yb-E10253 人粘蛋白/粘液素5B(MUC5B)ELISA试剂盒 Human mucin / mucin- 5B (MUC5B) ELISA Kit
yb-E10254 人肠三叶因子(ITF)ELISA试剂盒 Human intestinal trefoil factor (ITF) ELISA kit
yb-E10255 人Dickkopf 1(DKK1)ELISA试剂盒 Human Dickkopf 1 (DKK1) ELISA Kit
yb-E10256 人激肽释放酶11(KLK 11)ELISA试剂盒 Human kallikrein 11 (KLK 11) ELISA kit
yb-E10257 人生长调节致癌基因γ/黑素瘤生激因子(GROγ/CXCL3/MGSA)ELISA试剂盒 Human growth regulating oncogene γ / melanoma growth stimulating factor (GROγ/CXCL3/MGSA) ELISA Kit
yb-E10258 人生长调节致癌基因β/黑素瘤生激因子(GROβ/CXCL2/MGSA)ELISA试剂盒 Oncogenic human growth regulating β / melanoma growth stimulating factor (GROβ/CXCL2/MGSA) ELISA Kit
yb-E10259 人美丽线虫凋亡基因(CED-3)ELISA试剂盒 Beautiful people nematode apoptotic genes (CED-3) ELISA kit
yb-E10260 人胸腺白血病抗原(TLa)ELISA试剂盒 Human thymus leukemia antigen (TLa) ELISA kit
yb-E10261 人肿瘤特异性移植抗原(TSTA)ELISA试剂盒 Human tumor specific transplantation antigen (TSTA) ELISA Kit
yb-E10262 人足细胞标记蛋白/足盂蛋白(PCX)ELISA试剂盒 Human podocyte marker protein / protein spoon foot (PCX) ELISA Kit
yb-E10263 人乳腺癌易感蛋白1(BRCA-1)ELISA试剂盒 Human breast cancer susceptibility protein 1 (BRCA-1) ELISA Kit
yb-E10264 人T细胞急性淋巴母细胞白血病相关抗原(TALLA-1/CD231)ELISA试剂盒 Human T -cell acute lymphoblastic leukemia -associated antigen (TALLA-1/CD231) ELISA kit
yb-E10265 人核仁形成区嗜银蛋白(Ag-NORs)ELISA试剂盒 People nucleolar organizer regions associated proteins (Ag-NORs) ELISA kit
yb-E10266 人硫氧化还原蛋白(Trx)ELISA试剂盒 Human thioredoxin (Trx) ELISA kit
yb-E10267 人窖蛋白(Cav-1)ELISA试剂盒 Human caveolin (Cav-1) ELISA Kit
yb-E10268 人普通急性淋巴细胞白血病抗原(CALLA)ELISA试剂盒 Ordinary acute lymphoblastic leukemia antigen (CALLA) ELISA kit
yb-E10269 人黑色素细胞刺激素(MSH)ELISA试剂盒 Human melanocyte stimulating hormone (MSH) ELISA kit
yb-E10270 人表皮角蛋白(EK)ELISA试剂盒 Human epidermal keratin (EK) ELISA Kit
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
说明诊断试验阳性与阴性结果均正确的百分比，亦反映试验的真实性。 3)约登指数（youden's index）=A/(A+C)+D/(B+D)-1约登指数是将灵敏度与特异度之 和减1，指数可从0～1。约登指数愈大，其真实性亦愈大。 ②可靠性（reliability）：可靠性是指试验在相同条件下重复试验获得相同结果的稳定 程度。如试验结果为均数，可用变异系数（coefficient of Variation）表示。公式为 CV=S/X×100% S为标准差，X为平均值 ③预示
) ELISA 48T/96T 2000/3000 进口分装 F10251 小鼠 C 反应蛋白 (mouse CRP) ELISA 48T/96T 1500/2500 进口分装 F10252 小鼠 CXC 趋化因子受体 3(mouse CXCR3) ELISA 48T/96T 1800/2800 进口分装 F10253 小鼠 CXC 趋化因子配体 16(mouse CXCL16) ELISA 48T/96T 1800/2800 进口分装 F10254 小鼠 CXCL1/KC(mouse
饥饿的 HeLa 和 NIH 3T3 癌细胞系获得的裂解物中的磷酸化蛋白特异性。图示：从复杂生物样品中富集高纯度磷蛋白。用 theThermo Scientific Pierce 磷酸化蛋白富集试剂盒进行 Weste blot 分析，根据试剂盒说明书制备细胞裂解液，富集磷酸化蛋白。利用识别生长因子信号传导相关关键调控蛋白的磷酸化特异性抗体实现蛋白检测。细胞色素 C (pI 9.6) 和 p15Ink4b (pI 5.5) 作为非磷酸化蛋白非特异性结合的阴性对照。FT = 流穿馏分，W = 合并洗脱馏分，E
技术资料暂无技术资料 索取技术资料
趋化因子C-X-C-基元配体16(CXCL16)检测试剂盒
¥900 - 1890
