- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA899Hu
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
骨桥素(OPN)检测试剂盒
检测范围 0.625-40ng/mL 灵敏度 0.261ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
骨桥素(OPN)检测试剂盒
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骨桥素(OPN)检测试剂盒
检测范围 0.625-40ng/mL 灵敏度 0.261ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
骨桥素(OPN)检测试剂盒
ELISA Kit for Osteopontin (OPN)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA899Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.625-40ng/mL The standard curve concentrations used for the ELISA’s were 40ng/mL, 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.261ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Osteopontin (OPN).
No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.
No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.
Recovery
骨桥素(OPN)检测试剂盒Matrices listed below were spiked with certain level of recombinant Osteopontin (OPN) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteopontin (OPN) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-99 | 96 |
| EDTA plasma(n=5) | 96-103 | 101 |
| heparin plasma(n=5) | 78-104 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteopontin (OPN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteopontin (OPN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-94% | 78-98% | 97-104% | 79-105% |
| EDTA plasma(n=5) | 88-97% | 93-101% | 78-99% | 83-101% |
| heparin plasma(n=5) | 84-97% | 78-96% | 91-99% | 92-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
骨桥素(OPN)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteopontin (OPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteopontin (OPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验相关实验
大鼠 骨桥素(OPN ) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本 中 骨桥素(OPN ) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 大鼠 骨桥素(OPN ) 水 平。用纯化的 大鼠 骨桥素(OPN ) 抗体 包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 骨桥素(OPN ) ，再与 HRP 标记
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人骨桥素 ( OPN )ELISA 试剂盒 ( 用于母乳、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 OPN 单抗包被于酶标板上，标准品和样品中的 OPN与单抗结合，加入生物素化的抗人 OPN ，形成免疫复合物连接
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 豚鼠骨桥素 ( OPN )ELISA 试剂盒 ( 用于母乳、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗豚鼠 OPN 单抗包被于酶标板上，标准品和样品中的 OPN与单抗结合，加入生物素化的抗豚鼠 OPN ，形成
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骨桥素(OPN)检测试剂盒
¥900 - 1890
