- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA029Hu
- 2025年07月16日
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- 详细信息
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
E选择素(SELE)检测试剂盒
检测范围 39.06-2500pg/mL 灵敏度 14pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
E选择素(SELE)检测试剂盒
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yb-E10193 人碱性成纤维细胞生长因子9(bFGF-9)ELISA试剂盒 Human basic fibroblast growth factor 9 (bFGF-9) ELISA kit
yb-E10194 人碱性成纤维细胞生长因子6(bFGF-6)ELISA试剂盒 Human basic fibroblast growth factor 6 (bFGF-6) ELISA Kit
yb-E10195 人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒 Human basic fibroblast growth factor 4 (bFGF-4) ELISA kit
yb-E10196 人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒 Human acidic fibroblast growth factor 1 (aFGF-1) ELISA Kit
yb-E10197 人凋亡相关因子配体(FASL)ELISA试剂盒 Apoptosis -related factor ligand (FASL) ELISA kit
yb-E10198 人凋亡相关因子(FAS/CD95)ELISA试剂盒 Apoptosis -related factors (FAS/CD95) ELISA kit
yb-E10199 人E选择素(E-Selectin/CD62E)ELISA试剂盒 Human E -selectin (E-Selectin/CD62E) ELISA kit
yb-E10200 人嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA试剂盒 Human eosinophil chemoattractant protein Eotaxin 1 (Eotaxin 1/CCL11) ELISA kit
yb-E10201 人鼠嗜酸粒细胞趋化因子(ECF)ELISA试剂盒 Of Mice and eosinophil chemotactic factor (ECF) ELISA kit
yb-E10202 人内分泌腺来源的血管内皮生长因子(EG-VEGF)ELISA试剂盒 The human endocrine origin VEGF (EG-VEGF) ELISA Kit
yb-E10203 人睫状神经营养因子(CNTF)ELISA试剂盒 Human ciliary neurotrophic factor (CNTF) ELISA kit
yb-E10204 人CD30分子(CD30)ELISA试剂盒 Human CD30 molecule (CD30) ELISA Kit
yb-E10205 人CXC趋化因子受体1(CXCR1)ELISA试剂盒 Human CXC chemokine receptor 1 (CXCR1) ELISA kit
yb-E10206 人XC趋化因子受体1(XCR1)ELISA试剂盒 People XC chemokine receptor 1 (XCR1) ELISA kit
yb-E10207 人二级淋巴组织趋化因子(SLC/CCL21)ELISA试剂盒 Human secondary lymphoid tissue chemokine (SLC/CCL21) ELISA kit
yb-E10208 人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒 Human E -cadherin / epithelial cadherin (E-Cad) ELISA kit
yb-E10209 人脑源性神经营养因子(BDNF)ELISA试剂盒 The human brain -derived neurotrophic factor (BDNF) ELISA kit
yb-E10210 人白细胞活化黏附因子(ALCAM)ELISA试剂盒 Activation of human leukocyte adhesion molecule (ALCAM) ELISA kit
yb-E10211 人活化素A(ACV-A)ELISA试剂盒 Human activin A (ACV-A) ELISA Kit
yb-E10212 人神经调节蛋白1(NRG-1)ELISA试剂盒 Human neuregulin 1 (NRG-1) ELISA Kit
yb-E10213 人心钠肽(ANP)ELISA试剂盒 People natriuretic peptide (ANP) ELISA kit
yb-E10214 人多巴胺D2受体(D2R)ELISA试剂盒 Human dopamine D2 receptor (D2R) ELISA Kit
yb-E10215 人内肽-2(EM-2)ELISA试剂盒 The people endomorphins -2 (EM-2) ELISA Kit
yb-E10216 人α-内肽(α-EP)ELISA试剂盒 Human α- endomorphins (α-EP) ELISA kit
E选择素(SELE)检测试剂盒
检测范围 39.06-2500pg/mL 灵敏度 14pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
E选择素(SELE)检测试剂盒
ELISA Kit for Selectin, Endothelium (SELE)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA029Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 39.06-2500pg/mL The standard curve concentrations used for the ELISA’s were 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.13pg/mL, 39.06pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 14pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Selectin, Endothelium (SELE).
No significant cross-reactivity or interference between Selectin, Endothelium (SELE) and analogues was observed.
No significant cross-reactivity or interference between Selectin, Endothelium (SELE) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Selectin, Endothelium (SELE) and the recovery rates were calculated by comparing the measured value to the expected amount of Selectin, Endothelium (SELE) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-93 | 87 |
| EDTA plasma(n=5) | 81-98 | 92 |
| heparin plasma(n=5) | 81-96 | 89 |
Precision
E选择素(SELE)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selectin, Endothelium (SELE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selectin, Endothelium (SELE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selectin, Endothelium (SELE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Selectin, Endothelium (SELE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-93% | 78-96% | 98-105% | 80-93% |
| EDTA plasma(n=5) | 97-104% | 89-96% | 80-95% | 81-101% |
| heparin plasma(n=5) | 80-99% | 87-103% | 78-103% | 88-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
E选择素(SELE)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Endothelium (SELE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selectin, Endothelium (SELE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selectin, Endothelium (SELE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selectin, Endothelium (SELE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10192 人趋化因子(fractalkine/CX3CL1) ELISA试剂盒 Human chemokine (fractalkine/CX3CL1) ELISA Kit
yb-E10193 人碱性成纤维细胞生长因子9(bFGF-9)ELISA试剂盒 Human basic fibroblast growth factor 9 (bFGF-9) ELISA kit
yb-E10194 人碱性成纤维细胞生长因子6(bFGF-6)ELISA试剂盒 Human basic fibroblast growth factor 6 (bFGF-6) ELISA Kit
yb-E10195 人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒 Human basic fibroblast growth factor 4 (bFGF-4) ELISA kit
yb-E10196 人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒 Human acidic fibroblast growth factor 1 (aFGF-1) ELISA Kit
yb-E10197 人凋亡相关因子配体(FASL)ELISA试剂盒 Apoptosis -related factor ligand (FASL) ELISA kit
yb-E10198 人凋亡相关因子(FAS/CD95)ELISA试剂盒 Apoptosis -related factors (FAS/CD95) ELISA kit
yb-E10199 人E选择素(E-Selectin/CD62E)ELISA试剂盒 Human E -selectin (E-Selectin/CD62E) ELISA kit
yb-E10200 人嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA试剂盒 Human eosinophil chemoattractant protein Eotaxin 1 (Eotaxin 1/CCL11) ELISA kit
yb-E10201 人鼠嗜酸粒细胞趋化因子(ECF)ELISA试剂盒 Of Mice and eosinophil chemotactic factor (ECF) ELISA kit
yb-E10202 人内分泌腺来源的血管内皮生长因子(EG-VEGF)ELISA试剂盒 The human endocrine origin VEGF (EG-VEGF) ELISA Kit
yb-E10203 人睫状神经营养因子(CNTF)ELISA试剂盒 Human ciliary neurotrophic factor (CNTF) ELISA kit
yb-E10204 人CD30分子(CD30)ELISA试剂盒 Human CD30 molecule (CD30) ELISA Kit
yb-E10205 人CXC趋化因子受体1(CXCR1)ELISA试剂盒 Human CXC chemokine receptor 1 (CXCR1) ELISA kit
yb-E10206 人XC趋化因子受体1(XCR1)ELISA试剂盒 People XC chemokine receptor 1 (XCR1) ELISA kit
yb-E10207 人二级淋巴组织趋化因子(SLC/CCL21)ELISA试剂盒 Human secondary lymphoid tissue chemokine (SLC/CCL21) ELISA kit
yb-E10208 人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒 Human E -cadherin / epithelial cadherin (E-Cad) ELISA kit
yb-E10209 人脑源性神经营养因子(BDNF)ELISA试剂盒 The human brain -derived neurotrophic factor (BDNF) ELISA kit
yb-E10210 人白细胞活化黏附因子(ALCAM)ELISA试剂盒 Activation of human leukocyte adhesion molecule (ALCAM) ELISA kit
yb-E10211 人活化素A(ACV-A)ELISA试剂盒 Human activin A (ACV-A) ELISA Kit
yb-E10212 人神经调节蛋白1(NRG-1)ELISA试剂盒 Human neuregulin 1 (NRG-1) ELISA Kit
yb-E10213 人心钠肽(ANP)ELISA试剂盒 People natriuretic peptide (ANP) ELISA kit
yb-E10214 人多巴胺D2受体(D2R)ELISA试剂盒 Human dopamine D2 receptor (D2R) ELISA Kit
yb-E10215 人内肽-2(EM-2)ELISA试剂盒 The people endomorphins -2 (EM-2) ELISA Kit
yb-E10216 人α-内肽(α-EP)ELISA试剂盒 Human α- endomorphins (α-EP) ELISA kit
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E选择素(SELE)检测试剂盒
¥900 - 1890
