Stemgent® Mouse ES/iPS Cell Ch

Stemgent® Mouse ES/iPS Cell Characterization Set详细信息：
Product Overview



The Stemgent™ Mouse ES/iPS Cell Characterization Set is a specific and sensitive, stem cell-verified tool designed to analyze the undifferentiated and differentiated status of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells. The set includes the Stemgent™ AP Staining Kit to measure alkaline phosphatase (AP) activity, and three ES cell-verified Stemgent™ antibodies to detect the mouse ES/iPS cell surface stage-specific embryonic antigen-1 (SSEA-1), and the intracellular transcription factors, Nanog and Oct4. The Stemgent™ Mouse ES/iPS Cell Characterization Set is quality tested by monitoring the AP staining activity of mouse ES cells, and assessing the undifferentiated/differentiated cell states through the expression of SSEA-1, Oct4, and Nanog by immunocytochemistry (ICC) and/or flow cytometry (FC).

Stemgent® Mouse ES/iPS Cell Characterization SetMouse ES and iPS cells are of extensive research interest because of their capacity for unlimited proliferation in vitro and their unique differentiation potential for future therapeutic use1,2. While the undifferentiated/pluripotent state of ES cells can best be defined functionally, the identification and characterization of ES and iPS cells by their unique biomarker(s) are extremely important to assess the quality of the cells in culture and the efficiency of cell reprogramming. The undifferentiated state of ES cells and iPS cells can be characterized by a high level of AP expression along with the expression of the mouse pluripotency markers SSEA-1, Nanog, and Oct4.

Alkaline Phosphatase (AP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES cells or iPS cells are stained with AP, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless, making this a simple, sensitive, and rapid assay for the verification of the pluripotent state3,4.

Stemgent® Mouse ES/iPS Cell Characterization SetSSEA-1, a carbohydrate antigen, is expressed on the surface of preimplantation-stage mouse embryos at the eight-cell stage5. It is strongly expressed by undifferentiated mouse ES cells6. Upon differentiation, mouse ES cells are characterized by the loss of SSEA-1 expression7.

Both Nanog and Oct4 are transcription factors that have been associated with an undifferentiated phenotype in embryonic ES/iPS cell8-10. A critical amount of Nanog and Oct4 expression is required to sustain stem-cell pluripotency. These key factors form a regulatory network to support or limit each others expression level and maintain the properties of ES cells. When ES cells are induced to differentiate, Nanog and Oct4 are downregulated, which has proven to be essential for a proper and divergent developmental program11,12. Functionally, Nanog and Oct4 work together with other key reprogramming factors (Sox2, Lin28, Klf4 and C-Myc) to reprogram mouse and human fibroblasts and generate induced pluripotent stem (iPS) cells13-15.
Stemgent® Mouse ES/iPS Cell Characterization Set
xy-E10041 干扰素调节因子(IRF)ELISA试剂盒 检测范围
xy-E10042 淋巴毒素α(LTA)ELISA试剂盒 检测范围
xy-E10043 CC趋化因子受体1(CCR1)ELISA试剂盒 检测范围
xy-E10044 CX3C趋化因子受体1(CX3CR1)ELISA试剂盒 检测范围
xy-E10045 肺部活化调节趋化因子(PARC/CCL18)ELISA试剂盒 检测范围
xy-E10046 黏膜地址素细胞黏附分子(MAdCAM-1)ELISA试剂盒 检测范围
xy-E10047 β干扰素(IFN-β/IFNB)ELISA试剂盒 检测范围
xy-E10048 可溶性CD38(sCD38)ELISA试剂盒 检测范围
xy-E10049 可溶性CD21(CR2/sCD21)ELISA试剂盒 检测范围
xy-E10050 可溶性瘦素受体(sLR)ELISA试剂盒 检测范围
xy-E10051 Toll样受体9(TLR-9/CD289)ELISA试剂 检测范围
xy-E10052 转化生长因子β2(TGFβ2)ELISA试剂盒 检测范围
xy-E10053 单核细胞趋化蛋白4(MCP-4/CCL13)ELISA试剂盒 检测范围
xy-E10054 白三烯D4(LTD4)ELISA试剂盒 检测范围
xy-E10055 N钙黏蛋白/神经钙黏蛋白(N-Cad)ELISA试剂盒 检测范围
xy-E10056 红细胞刺激因子(ESF)ELISA试剂 检测范围
xy-E10057 肿瘤坏死因子相关激活诱导因子(TRANCE)ELISA试剂盒 检测范围
xy-E10058 生长激素释放因子(GH-RF)ELISA试剂盒 检测范围
xy-E10059 巨噬细胞趋化因子(MCF)ELISA试剂 检测范围
xy-E10060 α/β干扰素受体(IFN-α/βR)ELISA试剂盒 检测范围
xy-E10061 B细胞生长因子(BCGF)ELISA试剂盒 检测范围
xy-E10062 B细胞分化因子(BCDF)ELISA试剂盒 检测范围
xy-E10063 上皮细胞粘附分子(Ep-CAM/CD362)ELISA试剂盒 检测范围
xy-E10064 可溶性粘附分子(Sam)ELISA试剂盒 检测范围
xy-E10065 巨噬细胞替代激活相关化学因子1(AmAC-1)ELISA试剂盒 检测范围
xy-E10066 KU86抗体（KU86-Ab）ELISA试剂盒 检测范围
xy-E10067 8-异构前列腺素（8-isoprostane)ELISA试剂盒 检测范围
xy-E10068 硫代酸反应物(TBARS)ELISA试剂盒 检测范围
xy-E10069 L-Carnitine ELISA试剂盒 检测范围
xy-E10070 小反刍兽疫抗体(PPR Ab)ELISA试剂盒 检测范围
xy-E10071 副结核分枝杆菌抗体(M.pt Ab)ELISA试剂盒 检测范围
xy-E10072 结核分枝杆菌抗体（M.tuberculosis Ab）ELISA试剂盒 检测范围
xy-E10073 可溶性血管内皮生长因子受体2(VEGFR-2/sFLK-1)ELISA试剂盒 检测范围
xy-E10074 胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 检测范围
xy-E10075 穿孔素/成孔蛋白(PF/PFP)ELISA试剂盒 检测范围
xy-E10076 多效生长因子(PTN)ELISA试剂盒 检测范围
xy-E10077 可溶性CD28(sCD28)ELISA试剂盒 检测范围
xy-E10078 淋巴细胞因子ELISA试剂盒 检测范围
xy-E10079 胸腺活化调节趋化因子(TARC/CCL17)ELISA试剂盒 检测范围
xy-E10080 神经细胞粘附分子配体1(NCAM-L1/CD171)ELISA试剂盒 检测范围
xy-E10081 神经保护因子(CVNPF)ELISA试剂盒 检测范围