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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
E-Selectin Monoclonal Antibody
- 库存：
1
- 供应商：
上海信裕
- E-Selectin Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody against E Selectin Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone P2H3 Form Ascites Host Mouse Species Reactivity Human IsoType E-Selectin Monoclonal Antibody IgG1 [Ab] 1-3 mg/mL Specificity The P2H3 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs). P2H3, inhibits the adhesion of carcinoma cells and other cells to E-Selectin expressed in activated endothelial cells, and neutralizes their function. Vascular E-Selectin is expressed in activated endothelia, and mediates the interaction between leukocytes and endothelia during an inflammation response. Uses This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:1,000
- IF: 1:500
- IP: 1:150
Storage E-Selectin Monoclonal Antibody Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Kim JA, Territo MC, Wayner E, Carlos TM, Parhami F, Smith CW, Haberland ME, Fogelman AM, Berliner JA. Partial characterization of leukocyte binding molecules on endothelial cells induced by minimally oxidized LDL. Arterioscler Thromb 14(3):427-433, 1994. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - E-Selectin Monoclonal Antibody
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
人E-选择素(E-Selectin)ELISA试剂盒 说明书
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