This product is sold for laboratory research use only, not for human or in-vivo use.
Clone
P2H3
Form
Ascites
Host
Mouse
Species Reactivity
Human
IsoType
E-Selectin Monoclonal Antibody IgG1
[Ab]
1-3 mg/mL
Specificity
The P2H3 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs). P2H3, inhibits the adhesion of carcinoma cells and other cells to E-Selectin expressed in activated endothelial cells, and neutralizes their function. Vascular E-Selectin is expressed in activated endothelia, and mediates the interaction between leukocytes and endothelia during an inflammation response.
Uses
This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP).
Suggested Working Dilution
The optimal working dilution should be determined for each specific assay condition.
Western blot: 1:1,000
IF: 1:500
IP: 1:150
Storage
E-Selectin Monoclonal Antibody Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
References
Kim JA, Territo MC, Wayner E, Carlos TM, Parhami F, Smith CW, Haberland ME, Fogelman AM, Berliner JA. Partial characterization of leukocyte binding molecules on endothelial cells induced by minimally oxidized LDL. Arterioscler Thromb 14(3):427-433, 1994.
Warranty/Conditions
Covance products may not be resold or modified for resale without prior written approval.