Determination of the activity and GTPase specificity of uncharacterized GAPs.
Biochemical characterization of small GTPases and their associated GAPs.
Examination of the regulation of GAP activity by different cofactors or protein domains.
Screen the mutant protein of either GAPs or GTPases for activity and substrate specificity.
Identification of GAP inhibitors in HTS (high throughput screen) format. Please inquire for significant discounts on large quantities of any reagents in this kit.
RhoGAP assay Biochem KitIntroduction The Ras superfamily of small GTPases (such as Ras, Rho, Rab, Arf and Ran proteins) serve as binary switches cycling between a GDP-bound “OFF state” and a GTP-bound “ON state”, which mediates signals downstream. In cells, the cycling between the two states is mainly controlled by two types of regulatory proteins: the activating Guanine nucleotide Exchange Factors (GEFs) and the inactivating GTPase Activating Proteins (GAPs).
Small G-proteins of the Ras superfamily typically have an inrinsic GTPase activity, meaning that after they get activated (GTP-bound) by GEFs, they eventually “turn themselves off”. The intrinsic GTPase rate, however, is normally very slow, so the “automatic” turning off process can take up to hours.
Because of the slow intrinsic GTPase activity in most Ras superfamily GTPases, their GAPs are important signaling molecules that function to terminate their signaling. GAPs function by binding to the active, GTP-bound, form of their target G-proteins and greatly stimulating the G-protein’s intrinsic GTPase activity, thereby quickly turning off the G-protein.
This GAP assay kit measures the amount of inorganic phosphate (Pi) that is produced as a result of G-protein dependent hydrolysis of GTP to GDP + Pi. The kit contains all the reagents needed for the assay including GTPases, a positive control RhoGAP, carefully optimized buffers and phosphate detection reagent. The assay can be performed in either 96-well plates or in 384-well plates. There is enough reagents for 80 assays (20 for each provided GTPase) in the 96-well format and 160 assays (40 for each provided GTPase) in the 384-well format
RhoGAP assay Biochem Kit xy-2127xy 5-HTR4(5 hydroxytryptamine serotonin receptor 4)5-羟色胺受体4抗原
xy-1993xy Muc2 peptide粘蛋白-2/上皮膜抗原2抗原
xy-0733xy ADAMTS7 peptide整合素样金属蛋白酶与凝血酶1型-7抗原
xy-0045xy AT (Angiotenogen)血管紧张素原(抗原)
xy-0526xy 5-LOX (5-lipoxygenase)5-脂氧合酶抗原
xy-0733xy-HRxy ADAMTS7 peptide/HRP辣根过氧化物酶标记整合素样金属蛋白酶与凝血酶1型-7抗原
xy-0167xy Apo-E (Apolipoprotein E)载脂蛋白E(抗原)
xy-0245xy IKI3 family protein
xy-0317xy ILK-1 (Integrin-linked protein kinase 1)整合素连接激酶-1
xy-0114xy Aromatase芳香化酶(抗原)
xy-2337xy SP-C peptide肺表面活性蛋白C抗原
xy-2353xy Mast Cell Chymase肥大细胞类糜蛋白酶1
xy-2373xy Ceruloplasmin peptide铜蓝蛋白抗原
xy-2394xy CKLF1趋化素样蛋白
xy-0055xy ART(Artemin)(抗原)
xy-1510xy AATK peptide细胞凋亡关联酪氨酸激酶抗原
xy-0261xy AQP-2 (aquaporin Protein-2)水通道蛋白-2(抗原)
xy-1627xy ABCA1(ATP binding cassette transporter A1)腺苷三磷酸结合盒转运体A1抗原
xy-2535xy TFPI/LACI组织因子途径抑制剂
xy-2540xy GLUT12葡萄糖转运蛋白12
xy-2558xy EGFRvIII表皮生长因子受体III型突变体抗原
xy-2570xy CD26CD26抗原
xy-1231xy ABCG1 peptide三磷酸腺苷结合盒亚家族G1抗原
xy-2804xy Cardiac Troponin T心肌特异性肌钙蛋白T
xy-0164xy BACE( ASP2 ) β分泌酶(抗原)
xy-0583xy ABL1(v-abl Abelson murine leukemia viral oncogene homolog 1)ABL1抗原
xy-2940xy FUCA1/Alpha L fucosidase I peptideα-L岩藻糖苷酶抗原
xy-0275xy Bassoon(BSN)
xy-0127xy Bax peptideBax(多肽抗原)
xy-0409xy-FITxy Leptin peptide/FITC荧光素FITC标记瘦素抗原
xy-0032xy Bcl-2(抗原)Bcl-2(多肽抗原)
xy-0248xy BDNF (Brain-derived Neurotrophin factor)脑源神经营养因子(脑衍化神经营养因子)(多肽片断抗原)
xy-2249xy ACA11 peptide拟南芥ACA11蛋白多肽抗原
RhoGAP assay Biochem Kitxy-2247xy AHA3 peptide植物蛋白AHA3抗原(拟南芥)
xy-0217xy bFGF碱性成纤维细胞生长因子(多肽抗原)