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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃保存
- 保质期:
6个月
- 英文名:
CML Competitive ELISA
- 库存:
1
- 供应商:
上海信裕
- CML Competitive ELISA详细信息:
-
Description: Carboxymethyl Lysine (CML) is an advanced glycation endproduct (AGE) formed by oxidative stress and glycosylation. AGEs, including CML, can accumulate in tissues causing inflammation. Excessive AGEs also reduce antioxidant defense, weaken immune systems, impair DNA repair mechanisms, and promote the accumulation of toxins and infections in tissues. CML adducts have been found to accumulate in many diseases including Alzheimers disease, diabetes, chronic obstructive pulmonary disease (COPD), accelerated periodontal disease, renal disease, and atherosclerosis.
Echelons CML Competitive ELISA is a competitive immunoassay that quantifies CML in biological samples. CML in the sample competes with plate-bound CML for antibody binding; a decreasing signal in the plate indicates an increasing CML concentration in the sample.
CML Competitive ELISA
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文献和实验相关专题 DAY 1 1. Coat Nunc immuno-module plates overnight, in usual manner. 2. Set up competition assay between antibody and competing substance :- Prepare a 1:2 serial dilution of the competitive substance
DAY 1 1. Coat Nunc immuno-module plates overnight, in usual manner. 2. Set up competition assay between antibody and competing substance :- Prepare a 1:2 serial dilution of the competitive substance from 480 to 0.4 ng/ml
一篇文献中的ELISA方法如下:Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
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