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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Penicillium citrinum Nuclease P1
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Penicillium citrinum Nuclease P1品名简称:
Recombinant Penicillium citrinum Nuclease P1 protein货号:
CSB-YP328459PEU规格:
20μg价格:
2616LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P24289别名:
Deoxyribonuclease P1 Endonuclease P1种属:
Penicillium citrinum蛋白长度:
Full Length来源:
Yeast分子量:
31.2 kDa表达区域:
1-270aa氨基酸序列:
WGALGHATVAYVAQHYVSPEAASWAQGILGSSSSSYLASIASWADEYRLTSAGKWSASLHFIDAEDNPPTNCNVDYERDCGSSGCSISAIANYTQRVSDSSLSSENHAEALRFLVHFIGDMTQPLHDEAYAVGGNKINVTFDGYHDNLHSDWDTYMPQKLIGGHALSDAESWAKTLVQNIESGNYTAQAIGWIKGDNISEPITTATRWASDANALVCTVVMPHGAAALQTGDLYPTYYDSVIDTIELQIAKGGYRLANWINEIHGSEIAK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Hydrolyzes only single-stranded DNA and RNA without apparent specificity for bases.功能2:
Hydrolyzes only single-stranded DNA and RNA without apparent specificity for bases.文献:
"Recognition of single-stranded DNA by nuclease P1: high resolution crystal structures of complexes with substrate analogs."Romier C., Dominguez R., Lahm A., Dahl O., Suck D.Proteins 32:414-424(1998)研究领域:
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文献和实验, respectively, using the E. coli F-factor plasmid replication and bacteriophage P1 plasmid origin to maintain largeness (100–250 kb). Genomic DNA is subjected to partial digestion with a restriction endonuclease in order to break DNA into clonable size and size
Design and Preparation of Sequence-Specific RNase P Ribozymes
The catalytic RNA subunit of Escherichia coli RNase P (M1 RNA) is a structure-specific endonuclease that cleaves pre-tRNA substrates in trans . By combining catalytic and substrate RNAs in one RNA molecule, self-cleaving ribozymes
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Communications 杂志上发表了研究论文 Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing。 该研究阐释了基因编辑过程中染色体易位产生的分子机制,设计和开发了能够抑制基因编辑过程中染色体易位和大片段缺失等染色体结构异常的产生、安全性大幅提高的新型基因编辑酶 Cas9TX! 图 2:来源 Nature Communications 3. Science Advances:果蝇的求偶之谜
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