• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Design and Preparation of Sequence-Specific RNase P Ribozymes

        互联网

        2411
        The catalytic RNA subunit of Escherichia coli RNase P (M1 RNA) is a structure-specific endonuclease that cleaves pre-tRNA substrates in trans . By combining catalytic and substrate RNAs in one RNA molecule, self-cleaving ribozymes have been created ( 13 ). These constructs were converted to sequence-specific endonucleases by deleting 5′-segments of the tethered tRNA ( 1 ) ( see Fig. 1 ). It has also been shown that by linking a guide sequence to the 3′-end of the M1 RNA, a sequence-specific ribozyme could be designed ( 2 ) ( see Fig. 2 ).
         
        Fig. 1.  Design of sequence specific Endo.P-based RNase P ribozymes. Arrows denote sites of cleavage within the exogenous substrate RNAs. The underlined sequences denote the positions of the forward and reverse primers for the PCR. Endo P3 RNA 1s a theoretical approach and has not been checked experimentally.

         
        Fig. 2.  Design of a sequence specific M1GS-based RNase P ribozyme. The arrow denotes the site of cleavage within the exogenous RNA substrate. The underlined sequences denote the sites for the forward and reverse primers for the PCR. The internal guide sequence is indicated by a series of nucleotides (N), complementary to the cleaved target sequence ( see also Note 3 ).
        Similar to Endo.P1 RNA, the internal guide sequence forms the 3′-half of a 7 bp acceptor stem, but in this case, no full-size tRNA structure is present (compare ref. 1 with ref. 2 ).

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序