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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
密封干燥保存
- 保质期:
6个月
- 英文名:
IL6-promoter luciferase reporter plasmid
- 库存:
1000
- 供应商:
钰博生物
IL6-Promoter荧光素酶报告基因(报告基因质粒)(IL6-Promoter luciferase reporter plasimd)是钰博生物自主研发的用于检测human IL6-Promoter激活水平为目的的报告基因。IL6(Interleukin 6)白细胞介素-6,简称白介素6,是一种重要的细胞炎性细胞因子,在炎性疾病的发生发展中起了重要的作用,常作为炎性反应的重要指标。
IL6-Promoter报告基因主要应用于Jak/Stat信号通路的研究等和human IL6-Promoter激活水平的检测等。
pGMIL6-Promoter-Lu是钰博生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了human IL6启动子,可以高灵敏度地检测human IL6-Promoter的激活水平。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,增加了质粒对转录因子结合的特异性。另外,由于质粒体积减小,使得IL6-Promoter报告基因质粒更易于转染。
IL6-promoter luciferase reporter plasmid(IL6-promoter-Luc荧光素酶报告基因质粒)
运输与保存方法
冰袋(wet ice)运输。收到产品后放到-20 ºC保存。
注意事项
本质粒未经钰博生物允许不得用于任何商业用途,也不得移交给订货人实验室以外的任何人或单位。
为了您的健康,实验操作时请穿实验服和带一次性手套。
使用说明
pGMIL6-promoter-Lu可以采用常规转染方法转染哺乳动物细胞。用荧光素酶检测试剂盒或双荧光素酶检测试剂盒进行检测。
IL6-promoter luciferase reporter plasmid(IL6-promoter-Luc荧光素酶报告基因质粒)参考文献
[1] OHara SP, et al. Cholangiocyte N-Ras protein mediates lipopolysaccharide-induced interleukin 6 secretion and proliferation. J Biol Chem. 286(35):30352-60 (2011).
[2] Xiao W, et al. Co-operative functions between nuclear factors NFkappaB and CCAT/enhancer-binding protein-beta (C/EBP-beta) regulate the IL-6 promoter in autocrine human prostate cancer cells. Prostate. 61(4):354-70 (2004).
[6] Wang DJ, et al. Regulation of the let-7a-3 promoter by NF-κB. PLoS One. 7(2):e31240 (2012).
[7] Tran TA, et al. Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor- kappa B. PLoS One. 6(8):e23660 (2011).
[8] Robbins D, et al. Imaging NF-κB signaling in mice for screening anticancer drugs. Methods Mol Biol. 716: 169- 77 (2011).
[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells. ChemBiol Interact. 159(2):117-28 (2006).
[10] Ozeki M, et al. Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD. Anticancer Res. 24(5A):2657-63 (2004).
IL6-promoter luciferase reporter plasmid(IL6-promoter-Luc荧光素酶报告基因质粒)yb0193 人正常前列腺上皮细胞 RWPE-1
yb0194 人正常前列腺基质永生化细胞 WPMY-1
yb0195 人前列腺癌细胞 22RV1
yb0196 人前列腺癌细胞 DU 145
yb0197 人前列腺癌细胞 LNCaP clone FGC
yb0198 人前列腺癌细胞 PC-3
yb0199 人宫颈癌细胞 HeLa
yb0200 人乳腺腺癌细胞 MDA-MB-415
yb0201 人乳腺腺癌细胞 MDA-MB-436
yb0202 人宫颈癌细胞 HeLa 229
yb0203 人子宫颈鳞癌细胞 SiHa
yb0204 人子宫颈癌 C-33 A
yb0205 人子宫颈表皮癌细胞 MS751
yb0206 人子宫颈表皮癌细胞 ME-180
yb0207 人子宫内膜癌细胞 RL95-2
yb0208 人宫颈癌肠转移细胞 Ca Ski
yb0209 人子宫鳞癌细胞(高分化) HCC 94 [HCC941122]
yb0210 人子宫内膜腺癌细胞 HEC-1-B
yb0211 人卵巢癌细胞 HO-8910
yb0212 人卵巢癌细胞 NIH:OVCAR-3
yb0213 人卵巢癌细胞 SK-OV-3
yb0214 人卵巢透明细胞癌 ES-2
yb0215 人高转移卵巢癌细胞 HO-8910PM
yb0216 人乳腺癌细胞 HCC1937
yb0217 人胎盘绒毛癌细胞 JAR
yb0218 人羊膜细胞 HA
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文献和实验Promoter Deletion Analysis Using a Dual-Luciferase Reporter System
of interest (e.g., cis -acting elements or enhancer) in the promoter. Luciferase genes have been widely used as reporter genes for their sensitivity and efficiency. Firefly and Renilla luciferases are two commonly used reporters, which oxidize
【求助】topflash/foplash的luciferase opening reading fream针对的就是firefly萤光素酶吗?
lope 请教各位大侠: 购买的UPSTATE的TOPFLASH/FOPFLASH质粒,准备做双荧光素酶报告基因检测,看文献上都只是用这个质粒和Renilla的载体共转染细胞,是否这个质粒本身就携带了Firefly萤光素酶呢?我看了说明书,只是说two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse
Application of the Firefly Luciferase Reporter Gene
the expression of the wild-type or in vitro mutagenized receptor, and the second contains a reporter gene linked to a hormone-responsive promoter. Application of the hormone or a related synthetic drug activates the reporter gene, and the effects of the mutations
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