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Human G-CSF

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  • ¥1200 - 14080
  • SinoBio
  • 国产
  • C002
  • 2025年11月08日
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    • 保存条件

      蛋白表达区间:Thr31-Pro204

    • 保质期

      See instructions

    • 英文名

      标签:Tag free

    • 库存

      表达系统:E.coli

    • 供应商

      上海经科化学科技有限公司

    • 规格

      10ug/50ug/500ug/1mg

    规格:10ug产品价格:¥1200.0
    规格:50ug产品价格:¥3520.0
    规格:500ug产品价格:¥9840.0
    规格:1mg产品价格:¥14080.0
    产品描述
    Recombinant Human Granulocyte Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Thr31-Pro204 is expressed.
     
    蛋白编号
    P09919-2
     
    产品别称
    Granulocyte Colony-Stimulating Factor; G-CSF; Pluripoietin; Filgrastim; Lenograstim; CSF3; C17orf33; GCSF
     
    分子量
    18.8 KDa
     
    表观分子量
    16 kDa, reducing conditions
     
    剂型描述
    Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
     
    内毒素
    < 0.01 EU/µg as determined by LAL test.
     
    纯度-SDS-PAGE
    Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
     
    纯度-SEC-HPLC
    Greater than 95% as determined by SEC-HPLC. (QC verified)
     
    生物活性-Cell Based Assay
    Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.03 ng/ml.
     
    复溶
    • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
    • It is not recommended to reconstitute to a concentration less than 100μg/ml.
    • Dissolve the lyophilized protein in distilled water.
    • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
     
    运输条件
    • The product is shipped at ambient temperature.
    • Upon receipt, store it immediately at the temperature listed below.
     
    保存条件
    • Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
    • Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
    • Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
     
    产品背景
    Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

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    图标文献和实验
    该产品被引用文献

    Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

    相关实验
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    • Hematopoietic Stem Cell Mobilization with Agents Other than G-CSF

      factor (G-CSF) are the preferred source for transplantation. G-CSF mobilization regimens, however, are associated with known morbidities and a significant number of normal donors and patient populations fail to mobilize sufficient numbers of hematopoietic

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    文献支持
    Human G-CSF
    ¥1200 - 14080