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- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥16896.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥11808.0 |
| 规格： | 50ug | 产品价格： | ￥4576.0 |
| 规格： | 10ug | 产品价格： | ￥1560.0 |
Recombinant Human Granulocyte Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Thr31-Pro204 is expressed.
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells
成员:Wang Xiaobin, Liu Xiaoyu, Wang Huihan
论文因子:5.854 发表期刊:CANCER GENE THERAPY pmid:35768562
| 基本信息 | |
| 基因名 | |
| 别名 | Granulocyte Colony-Stimulating Factor;G-CSF;Pluripoietin;Filgrastim;Lenograstim;CSF3;C17orf33;GCSF |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol,pH 4.0. |
| 纯度 | ED50 is less than 0.1ng/ml. Specific Activity of 6.0×107 IU/mg.measured by the dose-dependent proliferation of murine NFS-60 indicator cells.≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 区间范围 | MTPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLY QGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRV LRHLAQP |
| 背景说明 | Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds.It induces the survival，proliferation，and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils.Among the family of colony-stimulating factors，G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines.The synthesis of G-CSF can be induced by bacterial endotoxins，TNF，Interleukin-1，and GM-CSF.Prostaglandin E2 inhibits the synthesis of G-CSF.In epithelial，endothelial，and fibroblastic cells secretion of GCSF is induced by Interleukin-17. |
| 来源 | E.coli |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in16X PBS.Please aliquot the reconstituted solution to minimize freeze-t cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 IEU/ug). |
| 单位 | 支 |
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文献和实验【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells
成员:Wang Xiaobin, Liu Xiaoyu, Wang Huihan
论文因子:5.854 发表期刊:CANCER GENE THERAPY pmid:35768562
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
In this chapter we describe efficient procedures for the construction, expression and screening of comprehensive libraries of human Fab antibody fragments displayed on the surface of filamentous phage. Phagemid vectors are used for placing
我们知道，PeproTech的所有细胞因子和蛋白均为冻干粉，这使得运输非常便捷，只要常温即可。而且，细胞因子和蛋白冻干粉非常稳定，在-20℃或-80℃条件下可保存数年。冻干粉在使用前需进行溶解，然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键，因溶解不好会导致细胞因子或蛋白的失活，这也是很多用户在实际使用中经常遇到的问题。 那么，应该如何进行正确的溶解呢？ 下面我们以Recombinant Human IL-4 (重组人IL-4，产品编号：200-04)的说明书为例
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