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- 2025年12月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
蛋白表达区间:Gln28-Glu480
- 保质期：
See instructions
- 英文名：
标签:C-6His
- 库存：
表达系统:Human Cells
- 供应商：
上海经科化学科技有限公司
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ￥640.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥1760.0 |
| 规格： | 500ug | 产品价格： | ￥12320.0 |
| 规格： | 1mg | 产品价格： | ￥17600.0 |
- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
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文献和实验Inter-Cellular Adhesion Molecule 1 (ICAM1) is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. As an endothelial and leukocyte-associated transmembrane protein, ICAM1 is well known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration. The presence of heavy glycosylation and other structural characteristics lend ICAM1 binding sites for a number of immune-associated ligands. Notably, ICAM-1 binds to macrophage adhesion ligand-1 (Mac-1; ITGB2 / ITGAM), leukocyte function associated antigen-1 (LFA-1/integrin), and fibrinogen.ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.
Buffer. At same time strip the Human IgG column with 5 column bed volumes of Stripping Buffer. Equilibrate Human IgG column with 10 column bed volumes of Equilibration Buffer and store at 4o C. Before beginning elution step of R6.5 column, set
ICAM-1纯化 Purification of ICAM-1
Purification of ICAM-1 by Mazen Ferzly, 06/22/2000 Purpose The purification of ICAM-1 on R6.5 anti-ICAM-1 column. Materials Triethylamine NaCl Octyl Glucoside ( n-octyl-b -D-glucopyranoside Sigma Cat
ELISA FOR DETECTING SECRETED ICAM-1
. Add 50 ul of culture supernatant containing secreted ICAM-1. Include positive control wells of purified ICAM-1 ranging from 512 ng/ml to 2 ng/ml. Incubate 30 min at 37o C. Wash 4X with PBS. Add 50 ul/well Biotinylated R6.5 diluted
技术资料