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Caspase 3 Activity Assay roche详细信息：
Application
Samples suitable for the Caspase 3 Activity Assay are:
Lysates of apoptosis-induced cells
Recombinant caspase 3 (CPP32)
The Caspase 3 Activity Assay can be used to:
Detect and quantify in vitro caspase 3 activity in cell lysates
Analyze caspase 1, 2, 3, or 7 activity by simply omitting the capture-antibody step
Perform inhibitor studies for Caspase 3
This kit is intended as a tool for life-science research to further the understanding of apoptosis.
Benefits
Caspase 3 Activity Assay rocheSpecifically detect caspase 3 activity: Detect natural and recombinant human caspase 3 activity - not other caspases - using the caspase 3-specific anti-CPP32 monoclonal capture antibody
Achieve highly sensitive detection of caspase 3 activity: Detect extremely low levels of caspase 3 activity in lysates using the kit’s fluorescent substrate in an immunosorbent enzyme assay
Screen multiple samples simultaneously: Take advantage of the kit’s convenient 96-well microplate format when screening multiple samples
Store lysates for extended periods: Since the same buffer serves as both lysis and reaction buffers, you can immediately assay lysates or store them for subsequent analysis
Background Information
The initiation of all intracellular events seems to be characterized by the activation of specific proteases called caspases (cysteinyl-aspartic-acid-proteases). Biological function of Caspase 3 (CPP32, Yama, Apopain) activation seems to play a key role during the apoptotic process. Activated CPP32 is responsible for the cleavage, and therefore the breakdown, of several cellular components related to DNA repair and regulation. Cleavage sites of different caspases, including CPP32, are described.
Contents
Caspase 3 Activity Assay rocheCoating Buffer, 10x, 5 ml
Anti-Caspase-3, 20x, 500 µl monoclonal antibody from mouse
Blocking Buffer, ready-to-use, 25 ml
Incubation Buffer, 5x, 50 ml
DTT, 100x, 400 µl (1 M) stock solution
Substrate Solution Ac- DEVD-AFC, 20x, 650 µl (1 mM) stock solution in DMSO
AFC, 150 µl (1 mM) Stock Solution in DMSO
Positive Control, 250 µl lysate from apoptotic U937 cells
Microplate, 12 modules of 8 wells each
Adhesive Plate Cover Foils, 2 sheets
Principle
The Caspase 3 Activity Assay is a fluorometric, immunosorbent enzyme assay (FIENA) for the specific, quantitative in vitro determination of caspase 3 activity in microplates. The test principle is shown in Figure 1.
Caspase 3 is captured from cellular lysates by a monoclonal antibody. Following the washing step, substrate is added that is cleaved in proportion to the amount of activated caspase 3. Due to proteolytic cleavage of the substrate, free fluorescent AFC is generated. Free AFC is fluorometrically determined at lmax = 505 nm. The developed fluorochrome is proportional to the concentration of activated caspase 3, and can be quantified by a calibration curve. The assay time is approximately 5 hours.
Caspase 3 Activity Assay roche
xy1036 HDMEC-a 人真皮微血管内皮细胞-成人 Human Dermal Microvascular Endothelial Cells-adult 5 x 10^5 cells/vial ATCC
xy1037 HDVSMC "人真皮血管平滑肌细胞-新生儿
" Human Dermal Vascular Smooth Muscle Cells-neonate 5 x 10^5 cells/vial ATCC
xy1038 HEK-n 人表皮角质细胞--新生 Human Epidermal Keratinocytes-neonatal 5 x 10^5 cells/vial ATCC
xy1039 HEK-a 人表皮角质细胞--成人 Human Epidermal Keratinocytes-adult 5 x 10^5 cells/vial ATCC
xy1040 HKf 人表皮角质细胞--胎儿 Human Keratinocytes-fetal 5 x 10^5 cells/vial ATCC
xy1041 HEM-l 人表皮黑色素细胞--浅色素 Human Epidermal Melanocytes-light 5 x 10^5 cells/vial ATCC
xy1042 HEM-m 人表皮黑色素细胞--中色素 Human Epidermal Melanocytes-medium 5 x 10^5 cells/vial ATCC
xy1043 HEM-d 人表皮黑色素细胞--深色素 Human Epidermal Melanocytes-dark 5 x 10^5 cells/vial ATCC
xy1044 HM 人黑色素细胞--成人 Human Melanocytes-adult 5 x 10^5 cells/vial ATCC
xy1045 HEM-f 人黑色素细胞--胎儿 Human Epidermal Melanocytes-fetal 5 x 10^5 cells/vial ATCC
xy1046 HDF-f 人真皮成纤维细胞--胎儿 Human Dermal Fibroblasts-fetal 5 x 10^5 cells/vial ATCC
xy1047 HDF-n 人真皮成纤维细胞--新生 Human Dermal Fibroblasts-neonate 5 x 10^5 cells/vial ATCC
xy1048 HDF-a 人真皮成纤维细胞--成人 Human Dermal Fibroblasts-adult 5 x 10^5 cells/vial ATCC
xy1049 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial ATCC
xy1050 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 2 x 10^6 cells/vial ATCC
xy1051 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 5 x 10^6 cells/vial ATCC
xy1052 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial x 5 ATCC
xy1053 ATCC
xy1054 毛发细胞系统 ATCC
xy1055 HHDPC 人毛乳头细胞 Human Hair Dermal Papilla Cells 5 x 10^5 cells/vial ATCC
xy1056 HHGMC 人生发基质细胞 Human Hair Germinal Matrix Cells 5 x 10^5 cells/vial ATCC
xy1057 HHORSC 人毛囊外根鞘细胞 Human Hair Follicular Outer Root Sheath Cells 5 x 10^5 cells/vial ATCC
xy1058 HHIRSC 人毛囊内根鞘细胞 Human Hair Follicular Inner Root Sheath Cells 5 x 10^5 cells/vial ATCC
xy1059 HHFK 人毛囊角质细胞 Human Hair Folliclar Keratinocytes 5 x 10^5 cells/vial ATCC
xy1060 ATCC
xy1061 淋巴细胞系统 ATCC
xy1062 HLEC 人淋巴内皮细胞 Human Lymphatic Endothelial Cells 5 x 10^5 cells/vial ATCC
xy1063 HLF 人淋巴成纤维细胞 Human Lymphatic Fibroblasts 5 x 10^5 cells/vial ATCC
xy1064 HTEpiC 人扁桃体上皮细胞 Human Tonsil Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1065 HTF 人扁桃体成纤维细胞 "Human Tonsil Fibroblasts
" 5 x 10^5 cells/vial ATCC
xy1066 HTyF 人胸腺成纤维细胞 Human Thymus Fibroblasts 5 x 10^5 cells/vial ATCC
xy1067 口腔细胞系统 ATCC
xy1068 HOMEC 人口腔微血管内皮细胞 Human Oral Microvascular Endothelial Cells ATCC
xy1069 HOK 人口腔角质细胞 Human Oral Keratinocytes 5 x 10^5 cells/vial ATCC
Application
Samples suitable for the Caspase 3 Activity Assay are:
Lysates of apoptosis-induced cells
Recombinant caspase 3 (CPP32)
The Caspase 3 Activity Assay can be used to:
Detect and quantify in vitro caspase 3 activity in cell lysates
Analyze caspase 1, 2, 3, or 7 activity by simply omitting the capture-antibody step
Perform inhibitor studies for Caspase 3
This kit is intended as a tool for life-science research to further the understanding of apoptosis.
Benefits
Caspase 3 Activity Assay rocheSpecifically detect caspase 3 activity: Detect natural and recombinant human caspase 3 activity - not other caspases - using the caspase 3-specific anti-CPP32 monoclonal capture antibody
Achieve highly sensitive detection of caspase 3 activity: Detect extremely low levels of caspase 3 activity in lysates using the kit’s fluorescent substrate in an immunosorbent enzyme assay
Screen multiple samples simultaneously: Take advantage of the kit’s convenient 96-well microplate format when screening multiple samples
Store lysates for extended periods: Since the same buffer serves as both lysis and reaction buffers, you can immediately assay lysates or store them for subsequent analysis
Background Information
The initiation of all intracellular events seems to be characterized by the activation of specific proteases called caspases (cysteinyl-aspartic-acid-proteases). Biological function of Caspase 3 (CPP32, Yama, Apopain) activation seems to play a key role during the apoptotic process. Activated CPP32 is responsible for the cleavage, and therefore the breakdown, of several cellular components related to DNA repair and regulation. Cleavage sites of different caspases, including CPP32, are described.
Contents
Caspase 3 Activity Assay rocheCoating Buffer, 10x, 5 ml
Anti-Caspase-3, 20x, 500 µl monoclonal antibody from mouse
Blocking Buffer, ready-to-use, 25 ml
Incubation Buffer, 5x, 50 ml
DTT, 100x, 400 µl (1 M) stock solution
Substrate Solution Ac- DEVD-AFC, 20x, 650 µl (1 mM) stock solution in DMSO
AFC, 150 µl (1 mM) Stock Solution in DMSO
Positive Control, 250 µl lysate from apoptotic U937 cells
Microplate, 12 modules of 8 wells each
Adhesive Plate Cover Foils, 2 sheets
Principle
The Caspase 3 Activity Assay is a fluorometric, immunosorbent enzyme assay (FIENA) for the specific, quantitative in vitro determination of caspase 3 activity in microplates. The test principle is shown in Figure 1.
Caspase 3 is captured from cellular lysates by a monoclonal antibody. Following the washing step, substrate is added that is cleaved in proportion to the amount of activated caspase 3. Due to proteolytic cleavage of the substrate, free fluorescent AFC is generated. Free AFC is fluorometrically determined at lmax = 505 nm. The developed fluorochrome is proportional to the concentration of activated caspase 3, and can be quantified by a calibration curve. The assay time is approximately 5 hours.
Caspase 3 Activity Assay roche
xy1036 HDMEC-a 人真皮微血管内皮细胞-成人 Human Dermal Microvascular Endothelial Cells-adult 5 x 10^5 cells/vial ATCC
xy1037 HDVSMC "人真皮血管平滑肌细胞-新生儿
" Human Dermal Vascular Smooth Muscle Cells-neonate 5 x 10^5 cells/vial ATCC
xy1038 HEK-n 人表皮角质细胞--新生 Human Epidermal Keratinocytes-neonatal 5 x 10^5 cells/vial ATCC
xy1039 HEK-a 人表皮角质细胞--成人 Human Epidermal Keratinocytes-adult 5 x 10^5 cells/vial ATCC
xy1040 HKf 人表皮角质细胞--胎儿 Human Keratinocytes-fetal 5 x 10^5 cells/vial ATCC
xy1041 HEM-l 人表皮黑色素细胞--浅色素 Human Epidermal Melanocytes-light 5 x 10^5 cells/vial ATCC
xy1042 HEM-m 人表皮黑色素细胞--中色素 Human Epidermal Melanocytes-medium 5 x 10^5 cells/vial ATCC
xy1043 HEM-d 人表皮黑色素细胞--深色素 Human Epidermal Melanocytes-dark 5 x 10^5 cells/vial ATCC
xy1044 HM 人黑色素细胞--成人 Human Melanocytes-adult 5 x 10^5 cells/vial ATCC
xy1045 HEM-f 人黑色素细胞--胎儿 Human Epidermal Melanocytes-fetal 5 x 10^5 cells/vial ATCC
xy1046 HDF-f 人真皮成纤维细胞--胎儿 Human Dermal Fibroblasts-fetal 5 x 10^5 cells/vial ATCC
xy1047 HDF-n 人真皮成纤维细胞--新生 Human Dermal Fibroblasts-neonate 5 x 10^5 cells/vial ATCC
xy1048 HDF-a 人真皮成纤维细胞--成人 Human Dermal Fibroblasts-adult 5 x 10^5 cells/vial ATCC
xy1049 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial ATCC
xy1050 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 2 x 10^6 cells/vial ATCC
xy1051 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 5 x 10^6 cells/vial ATCC
xy1052 HDF-f-mt 人真皮成纤维细胞-胎儿霉素C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial x 5 ATCC
xy1053 ATCC
xy1054 毛发细胞系统 ATCC
xy1055 HHDPC 人毛乳头细胞 Human Hair Dermal Papilla Cells 5 x 10^5 cells/vial ATCC
xy1056 HHGMC 人生发基质细胞 Human Hair Germinal Matrix Cells 5 x 10^5 cells/vial ATCC
xy1057 HHORSC 人毛囊外根鞘细胞 Human Hair Follicular Outer Root Sheath Cells 5 x 10^5 cells/vial ATCC
xy1058 HHIRSC 人毛囊内根鞘细胞 Human Hair Follicular Inner Root Sheath Cells 5 x 10^5 cells/vial ATCC
xy1059 HHFK 人毛囊角质细胞 Human Hair Folliclar Keratinocytes 5 x 10^5 cells/vial ATCC
xy1060 ATCC
xy1061 淋巴细胞系统 ATCC
xy1062 HLEC 人淋巴内皮细胞 Human Lymphatic Endothelial Cells 5 x 10^5 cells/vial ATCC
xy1063 HLF 人淋巴成纤维细胞 Human Lymphatic Fibroblasts 5 x 10^5 cells/vial ATCC
xy1064 HTEpiC 人扁桃体上皮细胞 Human Tonsil Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1065 HTF 人扁桃体成纤维细胞 "Human Tonsil Fibroblasts
" 5 x 10^5 cells/vial ATCC
xy1066 HTyF 人胸腺成纤维细胞 Human Thymus Fibroblasts 5 x 10^5 cells/vial ATCC
xy1067 口腔细胞系统 ATCC
xy1068 HOMEC 人口腔微血管内皮细胞 Human Oral Microvascular Endothelial Cells ATCC
xy1069 HOK 人口腔角质细胞 Human Oral Keratinocytes 5 x 10^5 cells/vial ATCC
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Caspase 3 Activity Assay roche
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