大鼠CNTF蛋白

大鼠CNTF蛋白
产品信息
别称：
Ciliary Neurotrophic Factor
蛋白构建：
A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified.
种属：
Rat
表达宿主：
E. coli
大鼠CNTF蛋白产品状态&运输说明：
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.


质控检测
纯度：
> 92 % as determined by SDS-PAGE.
生物活性：
1. Measured by its binding ability in a functional ELISA. Immobilized rat CNTF (Cat: 80012-RNAE) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) (Cat: 80019-R08B) with a linear range of 1.56-50.0 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-40 ng/ml.
内毒素：
Please contact us for more information.
稳定性：
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端：
Met 1
大鼠CNTF蛋白分子量：
The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.

缓冲液：
Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.


使用指导
储存方法：
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶条件：
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.大鼠CNTF蛋白
xy1582 P/S 青霉素/链霉素溶液 Penicillin/Streptomycin Solution 100 ml
xy1583 AMS 抗真菌溶液 Antimycotic Solution 50 ml
xy1584 ABAMS 抗菌/抗霉菌溶液 Antibiotic/Antimycotic Solution 50 ml
xy1585 CCGW 细胞培养超纯水 Cell Culture Grade Water 500 ml
xy1586 MDS 肌细胞分离液 Myocyte Detachment Solution 100 ml
xy1587 BPE 牛脑垂体提取物 Bovine Pituitary Extract 25 mg
xy1588 BPE 牛脑垂体提取物 Bovine Pituitary Extract 100 mg
xy1589 ITS 胰岛素铁硒传递蛋白 Insulin-Transferrin-Selenium, 100x 10 ml
xy1590 L-Glu 左旋谷氨酰胺溶液 L-Glutamine Solution, 200 mM 100 ml
xy1591 NEAA 100X非必需氨基酸 100X Non-Essential Amino Acids 100ml
xy1592 HGL PCR 人胚层检测试剂盒 Human Germ Layer Detection Kit 50 reactions
xy1593 Ored 油红O染色试剂盒 Oil Red O Staining Kit 100 ml
xy1594 StemDS 人胚胎干细胞分离液 StemDS. Human Embryonic Stem Cell Dissociation Solution 100 ml
xy1595 BPF 牛血浆纤维粘连蛋白 Bovine Plasma Fibronectin 1 mg
xy1596 HCF 人细胞纤连蛋白 Human Cellular Fibronectin 100 μg
xy1597 BPV 牛血浆玻连蛋白 Bovine Plasma Vitronectin 100 μg
xy1598 rHSA 重组人血清白蛋白 "Recombinant Human Serum Albumin
" 1g
10g
50g
100g
xy1602 其他细胞外基质
xy1603 BPF 牛血浆纤维粘连蛋白 Bovine Plasma Fibronectin 1 mg
xy1604 CCCSCK 胶原I-细胞培养表面包被试剂盒 Collage I-Cell Culture Surface Coating Kit 10 mg
xy1605 PLL 多聚赖氨酸 Poly-L-Lysine 1 mg/ml 1 ml
xy1606 PLL 多聚赖氨酸 Poly-L-Lysine 10 mg/ml 1 ml
xy1607 人类胚胎干细胞/诱导多能干细胞
xy1608 STEMium 人胚胎干细胞无血清培养基 Human Pluripotent Stem Cell Growth Medium 500 ml
xy1609 STEMium-XF 无异种人胚胎干细胞生长培养基 Xeno-free Human Pluripotent Stem Cell Growth Medium 500 ml
xy1610 STEMium-ACF 无动物成分的人胚胎干细胞生长培养基 Animal Component-free Human Pluripotent Stem Cell Growth Medium 500 ml
xy1611 StemDS 人胚胎干细胞分离液 StemDS. Human Embryonic Stem Cell Dissociation Solution 100 ml
xy1612 StemCryo 人胚胎干细胞冻存液 Human Pluripotent Stem Cell Cryopreservation Medium 50ml
xy1613 StemCryo 人胚胎干细胞冻存液 Human Pluripotent Stem Cell Cryopreservation Medium, 5 x 10mL
xy1614 MEF 小鼠胚胎成纤维细胞 Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial