大鼠CNTF蛋白

大鼠CNTF蛋白
产品信息
别称：
Ciliary Neurotrophic Factor
蛋白构建：
A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified.
种属：
Rat
表达宿主：
E. coli
大鼠CNTF蛋白产品状态&运输说明：
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.


质控检测
纯度：
> 92 % as determined by SDS-PAGE.
生物活性：
1. Measured by its binding ability in a functional ELISA. Immobilized rat CNTF (Cat: 80012-RNAE) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) (Cat: 80019-R08B) with a linear range of 1.56-50.0 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-40 ng/ml.
内毒素：
Please contact us for more information.
稳定性：
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端：
Met 1
大鼠CNTF蛋白分子量：
The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.

缓冲液：
Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.


使用指导
储存方法：
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶条件：
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.大鼠CNTF蛋白
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