- 询价
- SignaGen
- 美国
- SignaGen
- 2026年01月03日
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- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 库存：
现货 大量
- 供应商：
美中清水湾生物科技有限公司
Description: Adenovirus is so far the most efficient gene delivery vector with nearly 100% efficiency for both dividing and non-dividing mammalian cells while AAV and lentivirus usually give 30~40% infection efficiency. The custom shRNA adenovirus service is to convert the unsurpassable efficiency of adenovirus to effective gene silencing on all mammalian cells for both in vitro or in vivo applications. Our proprietary Ad.MAX™ technology provides maximum shRNA adenovirus production and more flexibility to meet customers' needs. Ready-to-use deliverables are suitable for in vitro and in vivo applications.
If commercial shRNAs or siRNAs of your target gene are not available or non-functional, or your project requires special design for further development, we offer special shRNA design without additional charge!
Advantages:
- One-stop service: from shRNA design to packaging.
- Unsurpassable high infection efficiency.
- Quick turnaround time: 3 to 4 weeks.
- Cost-effective.
- Special design request acceptable, FREE!
Wide choice of Ad.MAX™ shuttle vectors for shRNA cloning:
Requirements: GenBank access #, sequence of gene of interest or shRNA is needed.
Deliverables: Total 2.0 ml at 2x1010~5x1011 PFU/ml shRNA adenovirus stock will be delivered.
Options:
- Wide choice of selection markers.
- Constitutive or inducible.
- Concentration for in vivo application.
- Packaging-only if you already have a construct.
- Re-order of additional volume at reduced cost.
- Control virus: vector-only or scrambled shRNA.
The most popular full and customized shRNA adenovirus construction services with Ad.MAX™ system are being listed below. We are offering discount for new customers. Please inquire with us for pricing for your special needs.
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文献和实验位点提供有效的重组 BLOCK-iT™ Lentiviral RNAi Expression System 在分裂和不分裂哺乳动物细胞或者动物模型中均可获得稳定的shRNA表达 ·长期、有效的RNAi分析－有效的慢病毒整合·简单的U6框重组－att位点使得从U6入门载体的转移快速而简单·有效的慢病毒转导－为动物模型、难于转染、原代和不分裂细胞类型提供可重复的结果
载体，PCR制备的siRNA表达框在细胞中表达产生siRNA。 1． 化学合成生产siRNA优点：方便，研究人员几乎不需要做什么工作。缺点：价格较贵，效率只有转录合成的shRNA的1/10-1/40，基因抑制持续时间短，对细胞毒性大，转染效率低，此外由于合成工艺上存在不可弥补的缺陷，此方法不能合成shRNA，不能纠正合成中产生的20%左右的碱基错误。适用于：建议不再采用此种合成方法。不适用于：基本上对目前所有的实验室来说均不适用。评价：☆☆☆☆☆ 2． 生物合成转录生产编码siRNA优点：价格较低，抑制
RNA PolyIII 聚合酶转录终止位点，且两端分别设计酶切位点。5. pSUPER 载体进行 BglII，HindIII 的双酶切。获得线性质粒载体。6. DNA 双链和病毒载体的连接。7. 连接产物的转化。8. 测序验证 ShRNA 序列是否连接到载体。9. 将质粒转染细胞验证 ShRNA 敲减效率。以上是 shRNA 的茎环引物设计方法，在构建其它慢病毒或者腺病毒质粒时，方法是通用的。但是，对于以下几种情况：（1）不同的质粒，（2）选用不同的限制性内切酶，（3）连接质粒时选用重组或者黏性
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