- 询价
- SignaGen
- 美国
- SignaGen
- 2026年01月06日
企业认证
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 库存：
现货 大量
- 供应商：
美中清水湾生物科技有限公司
Description: Adenovirus is so far the most efficient gene delivery vector with nearly 100% efficiency for both dividing and non-dividing mammalian cells while AAV and lentivirus usually give 30~40% infection efficiency. The custom shRNA adenovirus service is to convert the unsurpassable efficiency of adenovirus to effective gene silencing on all mammalian cells for both in vitro or in vivo applications. Our proprietary Ad.MAX™ technology provides maximum shRNA adenovirus production and more flexibility to meet customers' needs. Ready-to-use deliverables are suitable for in vitro and in vivo applications.
If commercial shRNAs or siRNAs of your target gene are not available or non-functional, or your project requires special design for further development, we offer special shRNA design without additional charge!
Advantages:
- One-stop service: from shRNA design to packaging.
- Unsurpassable high infection efficiency.
- Quick turnaround time: 3 to 4 weeks.
- Cost-effective.
- Special design request acceptable, FREE!
Wide choice of Ad.MAX™ shuttle vectors for shRNA cloning:
Requirements: GenBank access #, sequence of gene of interest or shRNA is needed.
Deliverables: Total 2.0 ml at 2x1010~5x1011 PFU/ml shRNA adenovirus stock will be delivered.
Options:
- Wide choice of selection markers.
- Constitutive or inducible.
- Concentration for in vivo application.
- Packaging-only if you already have a construct.
- Re-order of additional volume at reduced cost.
- Control virus: vector-only or scrambled shRNA.
The most popular full and customized shRNA adenovirus construction services with Ad.MAX™ system are being listed below. We are offering discount for new customers. Please inquire with us for pricing for your special needs.
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文献和实验位点提供有效的重组 BLOCK-iT™ Lentiviral RNAi Expression System 在分裂和不分裂哺乳动物细胞或者动物模型中均可获得稳定的shRNA表达 ·长期、有效的RNAi分析－有效的慢病毒整合·简单的U6框重组－att位点使得从U6入门载体的转移快速而简单·有效的慢病毒转导－为动物模型、难于转染、原代和不分裂细胞类型提供可重复的结果
RNA PolyIII 聚合酶转录终止位点，且两端分别设计酶切位点。5. pSUPER 载体进行 BglII，HindIII 的双酶切。获得线性质粒载体。6. DNA 双链和病毒载体的连接。7. 连接产物的转化。8. 测序验证 ShRNA 序列是否连接到载体。9. 将质粒转染细胞验证 ShRNA 敲减效率。以上是 shRNA 的茎环引物设计方法，在构建其它慢病毒或者腺病毒质粒时，方法是通用的。但是，对于以下几种情况：（1）不同的质粒，（2）选用不同的限制性内切酶，（3）连接质粒时选用重组或者黏性
腺病毒（adenovirus，Ad）最初由W. P. ROWE等人在人腺样体中分离得到并命名，这是一种直径为70-100nm、无包膜的双链DNA病毒。目前已知的人腺病毒血清型有50多种。其中基于人腺病毒5型（Ad5）改造的载体使用较为广泛，其特点是人为地删除了E1和E3基因，导致腺病毒失去复制能力和一定程度上降低免疫原性，使得Ad5成为一种广泛、安全、有效的基因运输载体。 根据重组腺病毒的特点，我们继续了解腺病毒在不同领域中的应用都有哪些 腺病毒除了用作基因
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