- ¥1980
- SignaGen
- 美国
- SL100568-C2C12
- 2026年01月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 英文名：
GenMute™ siRNA Transfection Reagent for C2C12 Cell
- 库存：
现货 大量
- 供应商：
美中清水湾生物科技有限公司
- 规格：
毫升
Description:
GenMute™ siRNA Transfection Reagent for C2C12 Cell is pre-optimized for transfecting siRNA to C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).
Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on C2C12 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: |
|
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol.pdf
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文献和实验of controls to ensure the validity of the data. The editors of Nature Cell Biology have recommended several controls (2). Two of these controls are: A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant
/BLAST . 3. Design appropriate controls. A complete siRNA experiment should include a number of controls to ensure the validity of the data. The editors of Nature Cell Biology have recommended several controls
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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