- 询价
- 上海钰博
- 进口/国产
- YB441Hu01
- 2025年07月14日
14 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Lysosomal Associated Membrane Protein 1 (LAMP1)
- 库存：
10000
- 供应商：
钰博生物
溶酶体关联膜蛋白1(LAMP1)重组蛋白
适用生物 Homo sapiens (Human，人)
溶酶体关联膜蛋白1(LAMP1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 54.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybC235Hu02 斑联蛋白(ZYX)重组蛋白 Recombinant Zyxin (ZYX) Homo sapiens (Human)
ybC418Mu02 过氧化氢酶(CAT)重组蛋白 Recombinant Catalase (CAT) Mus musculus (Mouse)
ybU807Ra01 含卷曲螺旋域蛋白60(CCDC60)重组蛋白 Recombinant Coiled Coil Domain Containing Protein 60 (CCDC60) Rattus norvegicus (Rat)
ybB154Ra02 纤维蛋白原α(FGα)重组蛋白 Recombinant Fibrinogen Alpha (FGa) Rattus norvegicus (Rat)
ybE784Ca01 膜联蛋白A4(ANXA4)重组蛋白 Recombinant Annexin A4 (ANXA4) Canis familiaris; Canine (Dog)
ybA891Hu02 花生四烯酸-15-脂加氧酶(ALOX15)重组蛋白 Recombinant Arachidonate-15-Lipoybgenase (ALOX15) Homo sapiens (Human)
ybB349Hu02 腺苷酸环化酶关联蛋白1(CAP1)重组蛋白 Recombinant Adenylyl Cyclase Associated Protein 1 (CAP1) Homo sapiens (Human)
ybC418Hu02 过氧化氢酶(CAT)重组蛋白 Recombinant Catalase (CAT) Homo sapiens (Human)
ybC915Hu02 成纤维细胞生长因子13(FGF13)重组蛋白 Recombinant Fibroblast Growth Factor 13 (FGF13) Homo sapiens (Human)
ybM132Hu01 REV1同源物(REV1)重组蛋白 Recombinant REV1 Homolog (REV1) Homo sapiens (Human)
ybA292Hu02 α-胞衬蛋白(SPTAN1)重组蛋白 Recombinant Alpha-Fodrin (SPTAN1) Homo sapiens (Human)
ybA663Hu02 Toll样受体2(TLR2)重组蛋白 Recombinant Toll Like Receptor 2 (TLR2) Homo sapiens (Human)
ybB355Mu02 花生四烯酸-5-脂加氧酶(ALOX5)重组蛋白 Recombinant Arachidonate-5-Lipoybgenase (ALOX5) Mus musculus (Mouse)
ybA221Mu03 转录因子20(TCF20)重组蛋白 Recombinant Transcription factor 20 (TCF20) Mus musculus (Mouse)
ybE784Po01 膜联蛋白A4(ANXA4)重组蛋白 Recombinant Annexin A4 (ANXA4) Sus scrofa; Porcine (Pig)
ybE784Po02 膜联蛋白A4(ANXA4)重组蛋白 Recombinant Annexin A4 (ANXA4) Sus scrofa; Porcine (Pig)
ybB134Ra01 黑色素瘤关联硫酸软骨素蛋白聚糖(MCSP)重组蛋白 Recombinant Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) Rattus norvegicus (Rat)
适用生物 Homo sapiens (Human，人)
溶酶体关联膜蛋白1(LAMP1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 54.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Lysosomal Associated Membrane Protein 1 (LAMP1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPB441Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 54.0kDa |
| Predicted isoelectric point | 6.6 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | Cell membrane; Lysosome membrane;Single-pass type I membrane protein. Endosome membrane; Late endosome. |
| Residues | Ala49~Ser297 (Accession # P11279) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
溶酶体关联膜蛋白1(LAMP1)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-AF SVNYDTKSGP KNMTFDLPSD ATVVLNRSSC GKENTSDPSL VIAFGRGHTL TLNFTRNATR YSVQLMSFVY NLSDTHLFPN ASSKEIKTVE SITDIRADID KKYRCVSGTQ VHMNNVTVTL HDATIQAYLS NSSFSRGETR CEQDRPSPTT APPAPPSPSP SPVPKSPSVD KYNVSGTNGT CLLASMGLQL NLTYERKDNT TVTRLLNINP NKTSASGSCG AHLVTLELHS EGTTVLLFQF GMNASSS
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-AF SVNYDTKSGP KNMTFDLPSD ATVVLNRSSC GKENTSDPSL VIAFGRGHTL TLNFTRNATR YSVQLMSFVY NLSDTHLFPN ASSKEIKTVE SITDIRADID KKYRCVSGTQ VHMNNVTVTL HDATIQAYLS NSSFSRGETR CEQDRPSPTT APPAPPSPSP SPVPKSPSVD KYNVSGTNGT CLLASMGLQL NLTYERKDNT TVTRLLNINP NKTSASGSCG AHLVTLELHS EGTTVLLFQF GMNASSS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
溶酶体关联膜蛋白1(LAMP1)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybE206Hu02 嘌呤霉素敏感性氨肽酶(PSA)重组蛋白 Recombinant Puromycin Sensitive Aminopeptidase (PSA) Homo sapiens (Human)
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybC235Hu02 斑联蛋白(ZYX)重组蛋白 Recombinant Zyxin (ZYX) Homo sapiens (Human)
ybC418Mu02 过氧化氢酶(CAT)重组蛋白 Recombinant Catalase (CAT) Mus musculus (Mouse)
ybU807Ra01 含卷曲螺旋域蛋白60(CCDC60)重组蛋白 Recombinant Coiled Coil Domain Containing Protein 60 (CCDC60) Rattus norvegicus (Rat)
ybB154Ra02 纤维蛋白原α(FGα)重组蛋白 Recombinant Fibrinogen Alpha (FGa) Rattus norvegicus (Rat)
ybE784Ca01 膜联蛋白A4(ANXA4)重组蛋白 Recombinant Annexin A4 (ANXA4) Canis familiaris; Canine (Dog)
ybA891Hu02 花生四烯酸-15-脂加氧酶(ALOX15)重组蛋白 Recombinant Arachidonate-15-Lipoybgenase (ALOX15) Homo sapiens (Human)
ybB349Hu02 腺苷酸环化酶关联蛋白1(CAP1)重组蛋白 Recombinant Adenylyl Cyclase Associated Protein 1 (CAP1) Homo sapiens (Human)
ybC418Hu02 过氧化氢酶(CAT)重组蛋白 Recombinant Catalase (CAT) Homo sapiens (Human)
ybC915Hu02 成纤维细胞生长因子13(FGF13)重组蛋白 Recombinant Fibroblast Growth Factor 13 (FGF13) Homo sapiens (Human)
ybM132Hu01 REV1同源物(REV1)重组蛋白 Recombinant REV1 Homolog (REV1) Homo sapiens (Human)
ybA292Hu02 α-胞衬蛋白(SPTAN1)重组蛋白 Recombinant Alpha-Fodrin (SPTAN1) Homo sapiens (Human)
ybA663Hu02 Toll样受体2(TLR2)重组蛋白 Recombinant Toll Like Receptor 2 (TLR2) Homo sapiens (Human)
ybB355Mu02 花生四烯酸-5-脂加氧酶(ALOX5)重组蛋白 Recombinant Arachidonate-5-Lipoybgenase (ALOX5) Mus musculus (Mouse)
ybA221Mu03 转录因子20(TCF20)重组蛋白 Recombinant Transcription factor 20 (TCF20) Mus musculus (Mouse)
ybE784Po01 膜联蛋白A4(ANXA4)重组蛋白 Recombinant Annexin A4 (ANXA4) Sus scrofa; Porcine (Pig)
ybE784Po02 膜联蛋白A4(ANXA4)重组蛋白 Recombinant Annexin A4 (ANXA4) Sus scrofa; Porcine (Pig)
ybB134Ra01 黑色素瘤关联硫酸软骨素蛋白聚糖(MCSP)重组蛋白 Recombinant Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) Rattus norvegicus (Rat)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
的。ATG5 在死亡肿瘤细胞中高表达，因此成为抗肿瘤治疗成功与否的重要指标，而 ATG12-ATG5-ATG16L 复合物的形成对自噬隔离膜的延伸是至关重要的。LC3 是 Atg8 在哺乳动物细胞中的同源物，LC3B-I(定位于胞浆) 与磷脂酰乙醇胺（PE）共价连接并插入到自噬体的双层膜中形成 LC3B II。LC3 偶联到自噬体上以及 LC3-I 转换成 LC3-II，已被用来作为自噬评价和测定的公认指标。自噬溶酶体形成自噬体与溶酶体的直接融合形成自噬溶酶体，形成过程中主要是 LAMP1、Rab
多种分子伴侣编码基因和通过激细胞内众多不同的分子伴侣的方法来实现。 2．在E.coli中表达真核膜蛋白和多亚基蛋白质复合物的难题上有待解决。 3． 蛋白质分泌到培养基中的真正和有效机制需要明了。目前已经有多种体系能够是重组蛋白质分泌到培养基中。其中有些是利用信号肽、融合伴侣和具有穿透能力的因子，这种因子能够引起外膜的破坏和有限的渗漏。而另外的策略是利用已经存在的分泌通道，来保证更高特异性的分泌。有关这方面的工作需要对E.coli中众多分泌通道的更多了解。 4． 赋予原核细胞诸如真核
毒性或限制宿主细胞的生长，选用可抑制的启动子则至关重要[27，28]。例如，轮状病毒的VP7蛋白能有效地杀死细胞，因此必须在严格控制的条件下表达[29]。但在某些情况下，启动子的严格性并不合理，因为即使最小量的基因产物也能由于其毒性而杀死细胞。如能灭活核糖体或破坏膜渗透压的分子对细胞来说是致死性的[30]。对宿主的毒性并不仅限于外源基因，某种自身蛋白的过量表达也能造成同样的结果。如编码外膜磷脂蛋白的traT基因[31]。另外，不完全抑制的表达系统会造成质粒的不稳定，细胞生长速度的下降和重组蛋白产量的降低
技术资料暂无技术资料 索取技术资料
溶酶体关联膜蛋白1(LAMP1)重组蛋白
询价
