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- 上海钰博
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- YB592Po01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Caspase 1 (CASP1)
- 库存：
10000
- 供应商：
钰博生物
胱天蛋白酶1(CASP1)重组蛋白
适用生物 Sus scrofa; Porcine (Pig，猪)
胱天蛋白酶1(CASP1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 21.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybB871Hu01 G组着色性干皮病偶联因子(XPG)重组蛋白 Recombinant Xeroderma Pigmentosum, Complementation Group G (XPG) Homo sapiens (Human)
ybB872Hu01 CD3d分子(CD3d)重组蛋白 Recombinant Cluster Of Differentiation 3d (CD3d) Homo sapiens (Human)
ybB873Ra01 CD19分子(CD19)重组蛋白 Recombinant Cluster Of Differentiation 19 (CD19) Rattus norvegicus (Rat)
ybB874Hu01 凝血因子Ⅶ(F7)重组蛋白 Recombinant Coagulation Factor VII (F7) Homo sapiens (Human)
ybB875Hu01 角蛋白13(KRT13)重组蛋白 Recombinant Keratin 13 (KRT13) Homo sapiens (Human)
ybB876Hu01 高迁移率族核小体结合域蛋白1(HMGN1)重组蛋白 Recombinant High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1) Homo sapiens (Human)
ybB877Hu01 叉头框蛋白P3(FOXP3)重组蛋白 Recombinant Forkhead Box Protein P3 (FOXP3) Homo sapiens (Human)
ybB878Hu01 凝血因子Ⅷ(F8)重组蛋白 Recombinant Coagulation Factor VIII (F8) Homo sapiens (Human)
ybB879Ra01 载脂蛋白F(APOF)重组蛋白 Recombinant Apolipoprotein F (APOF) Rattus norvegicus (Rat)
ybB880Hu01 周期素依赖性激酶7(CDK7)重组蛋白 Recombinant Cyclin Dependent Kinase 7 (CDK7) Homo sapiens (Human)
ybB882Ra01 成纤维细胞生长因子10(FGF10)重组蛋白 Recombinant Fibroblast Growth Factor 10 (FGF10) Rattus norvegicus (Rat)
ybB884Mu01 受磷蛋白(PLN)重组蛋白 Recombinant Phospholamban (PLN) Mus musculus (Mouse)
ybB886Hu01 血管紧张素转化酶2(ACE2)重组蛋白 Recombinant Angiotensin I Converting Enzyme 2 (ACE2) Homo sapiens (Human)
ybB889Hu01 周期素依赖性激酶2(CDK2)重组蛋白 Recombinant Cyclin Dependent Kinase 2 (CDK2) Homo sapiens (Human)
ybB890Hu01 载脂蛋白C3(APOC3)重组蛋白 Recombinant Apolipoprotein C3 (APOC3) Homo sapiens (Human)
ybB893Ra01 血管内皮生长因子受体3(VEGFR3)重组蛋白 Recombinant Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) Rattus norvegicus (Rat)
ybB895Hu01 甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)重组蛋白 Recombinant Mannose Associated Serine Protease 1 (MASP1) Homo sapiens (Human)
ybB897Ra01 胰岛素降解酶(IDE)重组蛋白 Recombinant Insulin Degrading Enzyme (IDE) Rattus norvegicus (Rat)
适用生物 Sus scrofa; Porcine (Pig，猪)
胱天蛋白酶1(CASP1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 21.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Caspase 1 (CASP1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Sus scrofa; Porcine (Pig) |
| Product No. | RPB592Po01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 21.4kDa |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Asn120~Asp297 (Accession # Q9N2I1) with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-N PVKPASSEPR GSLKLCPPDI AQRLWKEKSA EIYPIMGKSI RTRLALIICN TEFENLPRRD GADVDIRDMK ILLEDLGYSV DVRENLTASD MAIELKAFAA RPEHKSSDST FLVLMSHGIQ AGICGKKYSE EVPDVLEVNT VFQILNTLNC PSLKDKPKVI IIQACRGEKQ GVVWIKD
MGHHHHHHSGSEF-N PVKPASSEPR GSLKLCPPDI AQRLWKEKSA EIYPIMGKSI RTRLALIICN TEFENLPRRD GADVDIRDMK ILLEDLGYSV DVRENLTASD MAIELKAFAA RPEHKSSDST FLVLMSHGIQ AGICGKKYSE EVPDVLEVNT VFQILNTLNC PSLKDKPKVI IIQACRGEKQ GVVWIKD
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
胱天蛋白酶1(CASP1)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
胱天蛋白酶1(CASP1)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
胱天蛋白酶1(CASP1)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybB870Hu01 微管蛋白β(TUBβ)重组蛋白 Recombinant Tubulin Beta (TUBb) Homo sapiens (Human)
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybB871Hu01 G组着色性干皮病偶联因子(XPG)重组蛋白 Recombinant Xeroderma Pigmentosum, Complementation Group G (XPG) Homo sapiens (Human)
ybB872Hu01 CD3d分子(CD3d)重组蛋白 Recombinant Cluster Of Differentiation 3d (CD3d) Homo sapiens (Human)
ybB873Ra01 CD19分子(CD19)重组蛋白 Recombinant Cluster Of Differentiation 19 (CD19) Rattus norvegicus (Rat)
ybB874Hu01 凝血因子Ⅶ(F7)重组蛋白 Recombinant Coagulation Factor VII (F7) Homo sapiens (Human)
ybB875Hu01 角蛋白13(KRT13)重组蛋白 Recombinant Keratin 13 (KRT13) Homo sapiens (Human)
ybB876Hu01 高迁移率族核小体结合域蛋白1(HMGN1)重组蛋白 Recombinant High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1) Homo sapiens (Human)
ybB877Hu01 叉头框蛋白P3(FOXP3)重组蛋白 Recombinant Forkhead Box Protein P3 (FOXP3) Homo sapiens (Human)
ybB878Hu01 凝血因子Ⅷ(F8)重组蛋白 Recombinant Coagulation Factor VIII (F8) Homo sapiens (Human)
ybB879Ra01 载脂蛋白F(APOF)重组蛋白 Recombinant Apolipoprotein F (APOF) Rattus norvegicus (Rat)
ybB880Hu01 周期素依赖性激酶7(CDK7)重组蛋白 Recombinant Cyclin Dependent Kinase 7 (CDK7) Homo sapiens (Human)
ybB882Ra01 成纤维细胞生长因子10(FGF10)重组蛋白 Recombinant Fibroblast Growth Factor 10 (FGF10) Rattus norvegicus (Rat)
ybB884Mu01 受磷蛋白(PLN)重组蛋白 Recombinant Phospholamban (PLN) Mus musculus (Mouse)
ybB886Hu01 血管紧张素转化酶2(ACE2)重组蛋白 Recombinant Angiotensin I Converting Enzyme 2 (ACE2) Homo sapiens (Human)
ybB889Hu01 周期素依赖性激酶2(CDK2)重组蛋白 Recombinant Cyclin Dependent Kinase 2 (CDK2) Homo sapiens (Human)
ybB890Hu01 载脂蛋白C3(APOC3)重组蛋白 Recombinant Apolipoprotein C3 (APOC3) Homo sapiens (Human)
ybB893Ra01 血管内皮生长因子受体3(VEGFR3)重组蛋白 Recombinant Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) Rattus norvegicus (Rat)
ybB895Hu01 甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)重组蛋白 Recombinant Mannose Associated Serine Protease 1 (MASP1) Homo sapiens (Human)
ybB897Ra01 胰岛素降解酶(IDE)重组蛋白 Recombinant Insulin Degrading Enzyme (IDE) Rattus norvegicus (Rat)
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文献和实验相关实验
问：请问各位高手，我想让酵母蛋白酶Ａ失活，决定采用ＰＣＲ进行ＤＮＡ序列的突变，此法行的通吗？可有前人研究过？ 答：国外有了"蛋白酶缺失"的用于重组蛋白表达的菌株。国内也许也有，小龙对于上游不大懂，不知道自己构建蛋白酶缺失的酵母菌是否有技术上的难度。参见一篇综述《生命的化学》2003 年23 卷1 期CHEMISTRY OF L IFE 2003，23(1) 酵母表达系统及其应用研究。 假如只是为了降低重组蛋白表达时被水解的可能，在提取液中加入蛋白酶抑制剂如PMSF可以部分解决。
成分。5.优化表达条件（1）重组蛋白不表达或者表达量低。如果重组蛋白不表达（包含体和可溶蛋白都没有）通过改变诱导剂浓度，诱导温度，时间等都不表达的时候，然后尝试更换菌株、质粒载体。降低诱导后培养温度。重组蛋白表达得越慢，其折叠效果就越好，大肠杆菌在4℃时依然可以表达重组蛋白，而将培养温度降低至25-30℃就能显著改善蛋白折叠。此方法不适用于温度诱导的重组蛋白表达；减少诱导剂。诱导剂浓度可以降低至1/10；选用Lac渗透酶缺陷型菌株，如Tuner、Rosetta等，也有很好的效果。这类菌株能够使进入每个细胞
蛋白、含二硫键蛋白在体外成功复性。 包涵体形成的原因 重组蛋白在宿主系统中高水平表达时，无论是原核表达体系或真核表达体系甚至高等真核表达体系，都会形成包涵体[2]。主要因为在重组蛋白的表达过程中，缺乏某些蛋白质折叠过程中需要的酶和辅助因子，或环境不适，无法形成正确的次级键等原因形成的[3]。 1、 表达量过高，研究发现在低表达时很少形成包涵体，表达量越高越容易形成包涵体。原因可能是合成速度太快，以至于没有足够的时间进行折叠，二硫键不能正确配对，过多的蛋白间的非特异
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胱天蛋白酶1(CASP1)重组蛋白
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