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Sigma货号S6639浓缩缓冲液(SSC Buffer 2

0╳)上海睿安生物13611631389
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  • ￥1470.90
  • Sigma-Aldrich
  • made in USA
  • S6639
  • 2025年10月22日
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    上海睿安生物科技有限公司
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 保存条件：

      Room Temperature

    • 保质期：

      1年

    • 英文名：

      SSC Buffer 20╳

    • 库存：

      100⁺瓶

    • 供应商：

      上海睿安生物13611631389

    • CAS号：

      UNSPSC代码:41105319

    • 规格：

      1L/瓶

    Sigma货号S6639-1L浓缩缓冲液(SSC Buffer 20╳)上海睿安生物13611631389

    SSC 20╳浓缩缓冲液

    for Northern and Southern blotting, solution

    别名:20╳ SSC Buffer，SSC hybridization buffer，Saline-sodium citrate buffer

    说明

    一般描述:SSC(柠檬酸钠盐)缓冲液20倍浓缩液是Southern和Northern杂交过程的标准试剂。 它可用于严格控制杂交后洗涤步骤所用洗涤缓冲液。

    应用

    SSC缓冲液20倍浓缩液已用于
    • 用作原位杂交人特异性重复Alu序列的杂交缓冲液
    • 用作Southern杂交、小鼠基因组DNA斑点印迹和microRNA的Northern印迹的印迹膜洗涤缓冲液
    • 用作L1反转录转座子荧光原位杂交(FISH)的孵育缓冲液

    特点和优势

    • 方便的即用型液体
    • 在杂交中具有多种用途

    组分:SSC缓冲液20倍浓缩液由3M氯化钠、0.3M柠檬酸钠组成(pH7.0)。

    产品细节图片1

    同行评审论文(部分文献)

    Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
    Liu H, et al.
    Alzheimers Dement., 12(2), 130-143 (2016)
    L1 retrotransposon expression in circulating tumor cells
    Papasotiriou I, et al.
    Testing, 12(2), e0171466-e0171466 (2017)
    Spatial detection of fetal marker genes expressed at low level in adult human heart tissue.
    Michaela Asp et al.
    Scientific reports, 7(1), 12941-12941 (2017-10-13)
    Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
    Interaction of histone H1 with superhelical DNA. Sedimentation and electron microscopical studies at low salt concentration.
    M Böttger et al.
    Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
    Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
    Expression profiling of microRNA using oligo DNA arrays.
    Chang-Gong Liu et al.
    Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
    After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
    产品细节图片2产品细节图片3

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    图标文献和实验
    该产品被引用文献

    Sigma货号S6639-1L浓缩缓冲液(SSC Buffer 20╳)上海睿安生物13611631389

    SSC 20╳浓缩缓冲液

    for Northern and Southern blotting, solution

    别名:20╳ SSC Buffer，SSC hybridization buffer，Saline-sodium citrate buffer

    说明

    一般描述:SSC(柠檬酸钠盐)缓冲液20倍浓缩液是Southern和Northern杂交过程的标准试剂。 它可用于严格控制杂交后洗涤步骤所用洗涤缓冲液。

    应用

    SSC缓冲液20倍浓缩液已用于
    • 用作原位杂交人特异性重复Alu序列的杂交缓冲液
    • 用作Southern杂交、小鼠基因组DNA斑点印迹和microRNA的Northern印迹的印迹膜洗涤缓冲液
    • 用作L1反转录转座子荧光原位杂交(FISH)的孵育缓冲液

    特点和优势

    • 方便的即用型液体
    • 在杂交中具有多种用途

    组分:SSC缓冲液20倍浓缩液由3M氯化钠、0.3M柠檬酸钠组成(pH7.0)。

    引用文献图片1

    同行评审论文(部分文献)

    Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
    Liu H, et al.
    Alzheimers Dement., 12(2), 130-143 (2016)
    L1 retrotransposon expression in circulating tumor cells
    Papasotiriou I, et al.
    Testing, 12(2), e0171466-e0171466 (2017)
    Spatial detection of fetal marker genes expressed at low level in adult human heart tissue.
    Michaela Asp et al.
    Scientific reports, 7(1), 12941-12941 (2017-10-13)
    Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
    Interaction of histone H1 with superhelical DNA. Sedimentation and electron microscopical studies at low salt concentration.
    M Böttger et al.
    Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
    Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
    Expression profiling of microRNA using oligo DNA arrays.
    Chang-Gong Liu et al.
    Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
    After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
    引用文献图片2引用文献图片3
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    Sigma货号S6639浓缩缓冲液(SSC Buffer 20╳)上海睿安生物13611631389
    ￥1470.90