Sigma货号L4130-100mg现货LPS(脂多糖)13611631389上海睿安生物
Sigma货号L4130-500mg现货LPS(脂多糖)13611631389上海睿安生物
一般描述
本品是从大肠杆菌血清型O111:B4中提取的TCA。来源菌株由私人提供。该LPS血清型已被用于刺激B细胞并诱导人肝细胞的NOS。
包装:100mg、500mg in poly bottle
应用:脂多糖(LPS)是革兰氏阴性菌细胞壁的特征组分。LPS及其脂质A部分可通过能够识别常见的病原体相关分子模式(PAMP)的Toll样受体蛋白家族成员Toll样受体4(TLR4)刺激先天性免疫系统细胞。
生化/生理作用
脂多糖(LPS)位于膜的外层,并且暴露在无包膜菌株的细胞表面。它们有助于维持外层膜的完整性,并保护细胞免受胆汁盐和亲脂性抗生素的破坏。
制备说明
本品溶于水(5mg/ml)或细胞培养基(1mg/ml),得到浑浊的淡黄色溶液。以盐水为溶剂时,经过涡旋并加热至70-80 oC后,得到浓度更高但仍然浑浊的溶液(20mg/ml)。脂多糖分子可在各种溶剂中形成胶束。在水和磷酸盐缓冲盐水中,可观察到浑浊的溶液。使用有机溶剂不会得到更澄清的溶液。以甲醇为溶剂将得到含漂浮物的混浊悬浮液,而以水为溶剂将得到均匀的混浊溶液。
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