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Histopaque-1077淋巴细胞分离液Sigma货号1

0771上海睿安生物13611631389
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  • ￥569.46
  • Sigma-Aldrich
  • made in USA
  • Sigma货号10771
  • 2025年12月15日
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    上海睿安生物科技有限公司
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 保存条件：

      2-8°C

    • 保质期：

      1年

    • 英文名：

      Histopaque®-1077

    • 库存：

      100⁺瓶

    • 供应商：

      上海睿安生物13611631389

    • 规格：

      100ml/瓶

    Histopaque-1077淋巴细胞分离液Sigma货号10771上海睿安生物13611631389

    Histopaque-1077淋巴细胞分离液

    Frequently Asked Questions

    Live Chat and Frequently Asked Questions are available for this Product.

    General description

    A solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. This medium facilitates the recovery of large numbers of viable mononuclear cells.

    法律信息

    Histopaque注册商标Sigma-Aldrich Co. LLC

    性质

    Quality Level

    PREMIUM

    sterility

    sterile-filtered

    shelf life

    (Expiry date on the label.)

    impurities

    endotoxin, tested

    density

    1.077 g/mL

    storage temp.

    2-8°C

    一般描述

    Histopaque-1077是一种无菌、经内毒素检测的聚蔗糖及泛影钠溶液，并调整密度至1.077g/mL。这种即用型的培养基可有助于从小体积的全血中进行活性淋巴细胞和其他单核细胞的快速恢复。

    应用

    Histopaque-1077已用于

    • 外周血单核细胞(PBMC)以及骨髓单核细胞(BMMC)的分离
    • 通过离心进行单核细胞的纯化
    • 人类淋巴细胞的分离
    • 从全血中进行乳腺癌循环肿瘤细胞(CTC)的分离

    法律信息:Histopaque is a registered trademark of Sigma-Aldrich Co.LLC.产品细节图片1产品细节图片2

    同行评审论文(文献)

    Tumor-Derived Exosomes Induce the Formation of Neutrophil Extracellular Traps: Implications For The Establishment of Cancer-Associated Thrombosis.
    Ana C Leal et al.
    Scientific reports, 7(1), 6438-6438 (2017-07-27)
    Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular
    Evaluation of respiration with clark type electrode in isolated mitochondria and permeabilized animal cells.
    Silva AM and Oliveira PJ
    Methods in Molecular Biology, 810, 7-24 (2012)
    A genetically engineered adenovirus vector targeted to CD40 mediates transduction of canine dendritic cells and promotes antigen-specific immune responses in vivo.
    Erin E Thacker et al.
    Vaccine, 27(50), 7116-7124 (2009-09-30)
    Targeting viral vectors encoding tumor-associated antigens to dendritic cells (DCs) in vivo is likely to enhance the effectiveness of immunotherapeutic cancer vaccines. We have previously shown that genetic modification of adenovirus (Ad) 5 to incorporate CD40 ligand (CD40L) rather than
    Identification and Actions of the Maresin 1 Metabolome in Infectious Inflammation.
    Romain A Colas et al.
    Journal of immunology (Baltimore, Md. : 1950), 197(11), 4444-4452 (2016-11-02)
    Maresin 1 (MaR1) is an immunoresolvent that governs resolution of acute inflammation, and its local metabolism in the context of infectious inflammation is of interest. In this study, we investigated the MaR1 metabolome in infectious exudates and its bioactions in
    A novel pathogenic pathway of immune activation detectable before clinical onset in Huntington's disease.
    Maria Björkqvist et al.
    The Journal of experimental medicine, 205(8), 1869-1877 (2008-07-16)
    Huntington's disease (HD) is an inherited neurodegenerative disorder characterized by both neurological and systemic abnormalities. We examined the peripheral immune system and found widespread evidence of innate immune activation detectable in plasma throughout the course of HD. Interleukin 6 levels

    产品细节图片3

     
    产品细节图片4产品细节图片5产品细节图片6 产品细节图片7

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    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献

    Histopaque-1077淋巴细胞分离液Sigma货号10771上海睿安生物13611631389

    Histopaque-1077淋巴细胞分离液

    Frequently Asked Questions

    Live Chat and Frequently Asked Questions are available for this Product.

    General description

    A solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. This medium facilitates the recovery of large numbers of viable mononuclear cells.

    法律信息

    Histopaque注册商标Sigma-Aldrich Co. LLC

    性质

    Quality Level

    PREMIUM

    sterility

    sterile-filtered

    shelf life

    (Expiry date on the label.)

    impurities

    endotoxin, tested

    density

    1.077 g/mL

    storage temp.

    2-8°C

    一般描述

    Histopaque-1077是一种无菌、经内毒素检测的聚蔗糖及泛影钠溶液，并调整密度至1.077g/mL。这种即用型的培养基可有助于从小体积的全血中进行活性淋巴细胞和其他单核细胞的快速恢复。

    应用

    Histopaque-1077已用于

    • 外周血单核细胞(PBMC)以及骨髓单核细胞(BMMC)的分离
    • 通过离心进行单核细胞的纯化
    • 人类淋巴细胞的分离
    • 从全血中进行乳腺癌循环肿瘤细胞(CTC)的分离

    法律信息:Histopaque is a registered trademark of Sigma-Aldrich Co.LLC.引用文献图片1引用文献图片2

    同行评审论文(文献)

    Tumor-Derived Exosomes Induce the Formation of Neutrophil Extracellular Traps: Implications For The Establishment of Cancer-Associated Thrombosis.
    Ana C Leal et al.
    Scientific reports, 7(1), 6438-6438 (2017-07-27)
    Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular
    Evaluation of respiration with clark type electrode in isolated mitochondria and permeabilized animal cells.
    Silva AM and Oliveira PJ
    Methods in Molecular Biology, 810, 7-24 (2012)
    A genetically engineered adenovirus vector targeted to CD40 mediates transduction of canine dendritic cells and promotes antigen-specific immune responses in vivo.
    Erin E Thacker et al.
    Vaccine, 27(50), 7116-7124 (2009-09-30)
    Targeting viral vectors encoding tumor-associated antigens to dendritic cells (DCs) in vivo is likely to enhance the effectiveness of immunotherapeutic cancer vaccines. We have previously shown that genetic modification of adenovirus (Ad) 5 to incorporate CD40 ligand (CD40L) rather than
    Identification and Actions of the Maresin 1 Metabolome in Infectious Inflammation.
    Romain A Colas et al.
    Journal of immunology (Baltimore, Md. : 1950), 197(11), 4444-4452 (2016-11-02)
    Maresin 1 (MaR1) is an immunoresolvent that governs resolution of acute inflammation, and its local metabolism in the context of infectious inflammation is of interest. In this study, we investigated the MaR1 metabolome in infectious exudates and its bioactions in
    A novel pathogenic pathway of immune activation detectable before clinical onset in Huntington's disease.
    Maria Björkqvist et al.
    The Journal of experimental medicine, 205(8), 1869-1877 (2008-07-16)
    Huntington's disease (HD) is an inherited neurodegenerative disorder characterized by both neurological and systemic abnormalities. We examined the peripheral immune system and found widespread evidence of innate immune activation detectable in plasma throughout the course of HD. Interleukin 6 levels

    引用文献图片3

     
    引用文献图片4引用文献图片5引用文献图片6 引用文献图片7
    相关实验

    • 流式细胞术应用 | PBMC T 细胞受体表征检测

      方案是基于 One Study 研究以及多家专业临床研究型流式实验室共同设计完成的。在这里我们展示了正常人 PBMC 应用 DuraClone IM TCR 管在 CytoFLEX 上的检测结果。方法外周血单核细胞（PBMC）分离将 10 毫升全血用 1X PBS + 3 ％ FCS 稀释至 21 mL。 将 6 mL Ficoll（Sigma：Histopaque 1077 目录号 10771）加入三个 15 mL 锥形小瓶中。通过用 10 mL 移液管以非常陡的角度将 7 mL 稀释的血液加入

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    文献支持
    Histopaque-1077淋巴细胞分离液Sigma货号10771上海睿安生物13611631389
    ￥569.46