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Sigma货号H3149肝素钠盐(来源于猪肠粘膜)13611

631389上海睿安生物
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  • ¥7307.87
  • Sigma-Aldrich
  • made in USA
  • H3149
  • 2025年10月17日
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    • 详细信息
    • 技术资料
    • 保存条件

      Room Temperature

    • 保质期

      1年

    • 英文名

      heparin

    • 库存

      100⁺支

    • 供应商

      上海睿安生物13611631389

    • CAS号

      9041-08-1

    • 规格

      500000 UNITS/支

    Sigma货号H3149肝素钠盐(来源于猪肠粘膜)13611631389上海睿安生物

    一般描述:肝素主要用于推迟血液的凝固。它在凝血途径中可增强抗凝血酶介导的蛋白酶失活。

     
    肝素钠是一种来自糖胺聚糖家族的硫酸化多糖,在细胞培养、癌症生物学和药物发现研究中发挥着至关重要的作用。它对蛋白质和细胞相互作用的影响使其对于研究分子和细胞过程、肿瘤建模、探索结合相互作用以及作为支持细胞活力的细胞培养补充剂很有价值。


    应用

    • 来自猪肠粘膜的肝素钠盐已用于从人血浆中纯化sLRP(可溶性循环低密度脂蛋白受体相关蛋白-1)。
    • 它已被用作用于培养人脐静脉内皮细胞的M199培养基中的一种补充剂。
    • 它在血液分离的过程中被用作抗凝血剂。

    包装:10000、25000、50000、100000、250000、500000、1000000 units in poly bottle.

    特点和优势

    • 高纯度化合物适用于各种研究应用

    常见问题

    1. How is Heparin sodium salt from porcine intestinal mucosa, Product H3149, solubilized?

      Heparin sodium salt from porcine intestinal mucosa is soluble in deionized water at a concentrationi of 50 mg/mL.

    2. How much of Heparin sodium salt from porcine intestinal mucosa, Product H3149, is used for cell culture?

      Solutions at 0.5 mg per mL were tested in cell culture. Start at this concentration, and titer from there to determine the optimal concentration for usage.

    3. How are solutions Heparin sodium salt from porcine intestinal mucosa, Product H3149, stored?

      Solutions can be stored at 2-8C for up to 2 years, if sterile filtered through a 0.22 micron filter. Benzyl alcohol (0.06-1%) can be added to solutions to prevent microbial growth.

    4. How many milligrams of Heparin sodium salt, Product H3149, are in the bottle?

      The potency, in units/mg, is lot-specific; the value can be found on the certificate of analysis. It is typically over 140 units per mg. The number of milligrams can be calculated by dividing the number of units by the potency in units per mg.

    5. What is the molecular weight of Heparin sodium salt?

      Heparin is a mixture of polyanion chains having molecular weights ranging from 6,000 to 30,000 Daltons, with most chains in the range of 17,000 to 19,000 Daltons.

    6. How much Heparin sodium salt, is used to prevent coagulation?

      We suggest using 20 to 50 units per mL of blood.

    7. What is the best way to sterilize Heparin sodium salt?

      Although solutions may be autoclaved at 121 °C for 5 to 10 minutes, we generally recommend sterile filtration. This is because prolonged autoclaving may have an effect on its activity.  

    8. What concentration of Heparin sodium salt, can I use to inhibit RNAse activity?

      Heparin has been used at concentrations ranging from 100 to 500 μg/mL. This has been reported in the J. Biol. Chem., 248, 2095 (1973).

    Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

    同行评审论文(部分文献)

    Endothelial angiogenesis is directed by RUNX1T1-regulated VEGFA, BMP4 and TGF-β2 expression.
    Ko-Hsun Liao et al.
    PloS one, 12(6), e0179758-e0179758 (2017-06-24)
    Tissue angiogenesis is intimately regulated during embryogenesis and postnatal development. Defected angiogenesis contributes to aberrant development and is the main complication associated with ischemia-related diseases. We previously identified the increased expression of RUNX1T1 in umbilical cord blood-derived endothelial colony-forming cells
    Factor Xa and thrombin, but not factor VIIa, elicit specific cellular responses in dermal fibroblasts.
    Bachli EB et al.
    Journal of Thrombosis and Haemostasis, 1, 1935-1935 (2003)
    Effects of T-lymphocyte depletion on muscle fibrosis in the mdx mouse.
    Morrison J, et al.
    The American Journal of Pathology, 166, 1701-1701 (2005)
    Transcription factor ASCL2 is required for development of the glycogen trophoblast cell lineage.
    Aaron B Bogutz et al.
    PLoS genetics, 14(8), e1007587-e1007587 (2018-08-11)
    The basic helix-loop-helix (bHLH) transcription factor ASCL2 plays essential roles in diploid multipotent trophoblast progenitors, intestinal stem cells, follicular T-helper cells, as well as during epidermal development and myogenesis. During early development, Ascl2 expression is regulated by genomic imprinting and
    Structure of the antithrombin-binding site in heparin.
    Lindahl U et al.
    Proceedings of the National Academy of Sciences of the USA, 76, 3198-3202(1979)
    Sigma授权代理询订13611631389上海睿强生物&上海睿安生物产品细节图片1产品细节图片2产品细节图片3
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    一、试剂╳PBMC分离服务:PBMC/人外周血单个核细胞/PBMC细胞/人外周血单个核细胞/冻存PBMC/hPBMC单个核细胞
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    Thermo Fisher Scientific的很多产品线,包括旗下的:
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    产品细节图片5
    上海睿安生物科技有限公司成立于2009年,专注于为生命科学和生物技术领域的客户提供优质的产品与服务。代理了多家欧美知名品牌,如美国Thermo Fisher耗材(包括旗下Nunc、QSP、Nalgene、FisherBrand、Lab_Serv耗材仪器、Finnpipette移液器、Abgene耗材,等等)、默克Merck-Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)Zymo Research、洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材博奥龙Biodragon独家总代理EFL独家总代理碧云天Beyotime代理Eppendorf友康Yocon、Research Diets高脂饲料,IBL、BioVendor、Mercodia、ShiBayaji代谢类产品、Axygen-Corning长期大量耗材备货、BioCoat畅销款长期备货VivaCell血清、Access货号51HI血清、Gemini血清、Gibco血清,BI血清现货、ScienCell血清、Sigma血清、Moregate血清、Cegrogen血清、Pierce发光试剂、Fermentas、Invitrogen、ProteintechActive Motif、Abcam、CST、BD、SAB、R&D systemsBeckman、Qiagen、BD Pharmingen、Biolegend、Jackson、Miltenyi、MP Biomedicals、Biotium更好的荧光试剂产品等品牌。睿安生物始终将“提供更好的产品和服务”作为企业发展的目标和努力的方向,并将通过优质的售前与售后服务,始终如一地为客户提供高质量的产品和服务,我们也希望能得到相关领域中更多老师和同仁们的指导和鞭策,为共创优秀品牌而努力奋斗!询订直线13611631389微信号产品细节图片6

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