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Sigma货号A6014现货Acridine Orange吖

啶橙半(氯[lǜ]化锌)盐13611631389上海睿安生物
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  • ￥5107.50
  • Sigma-Aldrich
  • Made in USA
  • A6014
  • 2025年11月27日
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    上海睿安生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      Room temperature

    • 保质期：

      1年

    • 英文名：

      Acridine Orange

    • 库存：

      100⁺瓶

    • 供应商：

      上海睿安生物13611631389

    • CAS号：

      10127-02-3

    • 规格：

      100g/瓶

    Sigma货号A6014现货Acridine Orange吖啶橙半(氯[lǜ]化锌)盐13611631389上海睿安生物

    一般描述

    吖啶橙是一种异染性荧光阳离子染料，可渗透细胞膜并嵌入DNA和RNA。它能够实现在琼脂糖和聚丙[bǐng]烯酰胺凝胶上目视检测核酸。

    应用

    吖啶橙是一种细胞可渗透的异染色荧光阳离子染料，可嵌入DNA和RNA，用于荧光和表观显微镜检测。吖啶橙染料已被用于通过流式细胞术分析线粒体和溶酶体含量，表征多药耐药性以及检测大鼠胸腺细胞凋亡过程中线粒体质量的变化。
    适合

    • 检测凝胶电泳分离的核酸
    • 荧光和落射荧光显微镜
    • 流式细胞术分析线粒体和溶酶体
    • 凋亡研究中的DNA染色

    包装:10g、25g、100g in glass bottle。

    特点和优势

    • 120μM吖啶橙在单链和双链多核苷酸凝胶
    • 差异染色的每个条带中可检测到25-50ng的纯化DNA

    原理

    吖啶橙嵌入双螺旋核酸中，在530nm处可检测到绿色荧光。它与单链核酸中的磷酸基团静电结合，在640nm处可检测到红色荧光。
    产品细节图片1

    Sigma授权代理●询订13611631389上海睿强生物&上海睿安生物
    产品细节图片2 产品细节图片3
    产品细节图片4

    同行评审论文(Sigma货号A6014现货Acridine Orange吖啶橙半(氯[lǜ]化锌)盐:部分文献)

    Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    G K McMaster et al.
    Proceedings of the National Academy of Sciences of the United States of America, 74(11), 4835-4838 (1977-11-01)
    We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The
    Degradation of apoptotic cells and fragments in HL-60 suspension cultures after induction of apoptosis by camptothecin and ethanol.
    H Baisch et al.
    Cell proliferation, 32(5), 303-319 (2000-01-05)
    Early indicators of apoptosis in mammalian cells are membrane potential breakdown (loss) in mitochondria (MPLM), chromatin condensation, DNA degradation, and phosphatidylserine exposure (PSE) on the outside plasma membrane. One aim of the present study was to determine the kinetics of
    Synthesis of Bcl-2 in response to anthracycline treatment may contribute to an apoptosis-resistant phenotype in leukemic cell lines.
    F Durrieu et al.
    Cytometry, 36(2), 140-149 (1999-11-30)
    Some forms of chemoresistance in leukemia may start from failure of tumour cells to successfully undergo apoptosis and Bcl-2 may play a role in this defect. Therefore, we evaluated the Bcl-2 content and synthesis in relation with the apoptotic potential
    Features of apoptotic cells measured by flow cytometry.
    Z Darzynkiewicz et al.
    Cytometry, 13(8), 795-808 (1992-01-01)
    The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. Most of these methods were applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA
    Quick visualization of neurons in brain tissues using an optical clearing technique.
    Yu Sato et al.
    Anatomical science international, 94(2), 199-208 (2019-01-03)
    Neurons are classified into several morphological types according to the locations of their somata and the branching patterns of their axons and dendrites. Recent studies suggest that these morphological features are related to their physiological properties, including firing characteristics, responses

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    图标文献和实验
    该产品被引用文献

    Sigma货号A6014现货Acridine Orange吖啶橙半(氯[lǜ]化锌)盐13611631389上海睿安生物

    一般描述

    吖啶橙是一种异染性荧光阳离子染料，可渗透细胞膜并嵌入DNA和RNA。它能够实现在琼脂糖和聚丙[bǐng]烯酰胺凝胶上目视检测核酸。

    应用

    吖啶橙是一种细胞可渗透的异染色荧光阳离子染料，可嵌入DNA和RNA，用于荧光和表观显微镜检测。吖啶橙染料已被用于通过流式细胞术分析线粒体和溶酶体含量，表征多药耐药性以及检测大鼠胸腺细胞凋亡过程中线粒体质量的变化。
    适合

    • 检测凝胶电泳分离的核酸
    • 荧光和落射荧光显微镜
    • 流式细胞术分析线粒体和溶酶体
    • 凋亡研究中的DNA染色

    包装:10g、25g、100g in glass bottle。

    特点和优势

    • 120μM吖啶橙在单链和双链多核苷酸凝胶
    • 差异染色的每个条带中可检测到25-50ng的纯化DNA

    原理

    吖啶橙嵌入双螺旋核酸中，在530nm处可检测到绿色荧光。它与单链核酸中的磷酸基团静电结合，在640nm处可检测到红色荧光。
    引用文献图片1

    Sigma授权代理●询订13611631389上海睿强生物&上海睿安生物
    引用文献图片2 引用文献图片3
    引用文献图片4

    同行评审论文(Sigma货号A6014现货Acridine Orange吖啶橙半(氯[lǜ]化锌)盐:部分文献)

    Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    G K McMaster et al.
    Proceedings of the National Academy of Sciences of the United States of America, 74(11), 4835-4838 (1977-11-01)
    We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The
    Degradation of apoptotic cells and fragments in HL-60 suspension cultures after induction of apoptosis by camptothecin and ethanol.
    H Baisch et al.
    Cell proliferation, 32(5), 303-319 (2000-01-05)
    Early indicators of apoptosis in mammalian cells are membrane potential breakdown (loss) in mitochondria (MPLM), chromatin condensation, DNA degradation, and phosphatidylserine exposure (PSE) on the outside plasma membrane. One aim of the present study was to determine the kinetics of
    Synthesis of Bcl-2 in response to anthracycline treatment may contribute to an apoptosis-resistant phenotype in leukemic cell lines.
    F Durrieu et al.
    Cytometry, 36(2), 140-149 (1999-11-30)
    Some forms of chemoresistance in leukemia may start from failure of tumour cells to successfully undergo apoptosis and Bcl-2 may play a role in this defect. Therefore, we evaluated the Bcl-2 content and synthesis in relation with the apoptotic potential
    Features of apoptotic cells measured by flow cytometry.
    Z Darzynkiewicz et al.
    Cytometry, 13(8), 795-808 (1992-01-01)
    The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. Most of these methods were applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA
    Quick visualization of neurons in brain tissues using an optical clearing technique.
    Yu Sato et al.
    Anatomical science international, 94(2), 199-208 (2019-01-03)
    Neurons are classified into several morphological types according to the locations of their somata and the branching patterns of their axons and dendrites. Recent studies suggest that these morphological features are related to their physiological properties, including firing characteristics, responses
    相关实验

    • CELL CYCLE ANALYSIS

      sample to equilibrate and analyze for fluorescence immediately. top of page REAGENTS:Solution A:0.1 % Triton X-100, 0.08N HCl, 0.15M NaClSolution B:6 ug/ml Acridine Orange [Sigma, A 6014] , 1 mM EDTA-Na, 0.15M NaCl, 0.2M NaPO

    • 各等级BSA使用说明

      有DNAse酶、蛋白酶以及IgG，如Equitech bio公司货号为BAH67的BSA(我们公司长期有现货供应)和wako货号为：010-15131的牛血清蛋白。 作用：用于放射免疫、酶联免疫以及作为稀释剂或者封闭剂等。 5、 牛血清白蛋白(细胞培养级)，纯度98%即可，但是要求脂肪酸含量和内毒素含量极低，如：sigma货号为A8806的BSA(国外长期缺货，国内也没现货的)以及Equitech bio货号为BAH66的BSA。 作用：细胞培养及细胞培养相关的实验

    • 各等级BSA使用说明

      有DNAse酶、蛋白酶以及IgG，如Equitech bio公司货号为BAH67的BSA(我们公司长期有现货供应)和wako货号为：010-15131的牛血清蛋白。 作用：用于放射免疫、酶联免疫以及作为稀释剂或者封闭剂等。 5、 牛血清白蛋白(细胞培养级)，纯度98%即可，但是要求脂肪酸含量和内毒素含量极低，如：sigma货号为A8806的BSA(国外长期缺货，国内也没现货的)以及Equitech bio货号为BAH66的BSA。 作用：细胞培养及细胞培养相关的实验

    查看更多相关实验 >

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    文献支持
    Sigma货号A6014现货Acridine Orange吖啶橙半(氯[lǜ]化锌)盐13611631389上海睿安生物
    ￥5107.50