Sigma货号A6014现货Acridine Orange吖

Sigma货号A6014现货Acridine Orange吖啶橙半(氯[lǜ]化锌)盐13611631389上海睿安生物

一般描述

吖啶橙是一种异染性荧光阳离子染料，可渗透细胞膜并嵌入DNA和RNA。它能够实现在琼脂糖和聚丙[bǐng]烯酰胺凝胶上目视检测核酸。

应用

吖啶橙是一种细胞可渗透的异染色荧光阳离子染料，可嵌入DNA和RNA，用于荧光和表观显微镜检测。吖啶橙染料已被用于通过流式细胞术分析线粒体和溶酶体含量，表征多药耐药性以及检测大鼠胸腺细胞凋亡过程中线粒体质量的变化。
适合

• 检测凝胶电泳分离的核酸
• 荧光和落射荧光显微镜
• 流式细胞术分析线粒体和溶酶体
• 凋亡研究中的DNA染色

包装:10g、25g、100g in glass bottle。

特点和优势

• 120μM吖啶橙在单链和双链多核苷酸凝胶
• 差异染色的每个条带中可检测到25-50ng的纯化DNA

原理

吖啶橙嵌入双螺旋核酸中，在530nm处可检测到绿色荧光。它与单链核酸中的磷酸基团静电结合，在640nm处可检测到红色荧光。

同行评审论文(Sigma货号A6014现货Acridine Orange吖啶橙半(氯[lǜ]化锌)盐:部分文献)

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

G K McMaster et al.

Proceedings of the National Academy of Sciences of the United States of America, 74(11), 4835-4838 (1977-11-01)

We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The

Degradation of apoptotic cells and fragments in HL-60 suspension cultures after induction of apoptosis by camptothecin and ethanol.

H Baisch et al.

Cell proliferation, 32(5), 303-319 (2000-01-05)

Early indicators of apoptosis in mammalian cells are membrane potential breakdown (loss) in mitochondria (MPLM), chromatin condensation, DNA degradation, and phosphatidylserine exposure (PSE) on the outside plasma membrane. One aim of the present study was to determine the kinetics of

Synthesis of Bcl-2 in response to anthracycline treatment may contribute to an apoptosis-resistant phenotype in leukemic cell lines.

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Some forms of chemoresistance in leukemia may start from failure of tumour cells to successfully undergo apoptosis and Bcl-2 may play a role in this defect. Therefore, we evaluated the Bcl-2 content and synthesis in relation with the apoptotic potential

Features of apoptotic cells measured by flow cytometry.

Z Darzynkiewicz et al.

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The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. Most of these methods were applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA

Quick visualization of neurons in brain tissues using an optical clearing technique.

Yu Sato et al.

Anatomical science international, 94(2), 199-208 (2019-01-03)

Neurons are classified into several morphological types according to the locations of their somata and the branching patterns of their axons and dendrites. Recent studies suggest that these morphological features are related to their physiological properties, including firing characteristics, responses