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- C7661
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
1年
- 英文名：
Rat Collagen Type I(collagen from rat tail)
- 库存：
100⁺瓶
- 供应商：
上海睿安生物13611631389
- 规格：
100mg/瓶
Sigma货号C7661-5mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-10mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-25mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-50mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-100mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Rat Collagen Type I
from rat tail,powder,suitable for cell culture
说明
一般描述
所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成，其一级结构主要以三个氨基酸作为重复基序，即每隔两个氨基酸是一个甘氨酸残基，其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。I型胶原不同于其他胶原蛋白的方面为其低赖氨酸羟基化和低碳水化合物构成。Col1a1(胶原蛋白，I型，α1)或胶原蛋白是一种主要的人体结构蛋白，它以纤维的形式聚集。这是一种很长很薄的蛋白质，是人体中发现的最丰富的蛋白质。它是由三条左旋多肽链组成的超螺旋右螺旋结构。这些链由~1040个氨基酸组成，基本上是三个氨基酸-(Gly-X-Y)n的重复序列。Gly是甘氨酸，X和Y可以是任何氨基酸，但人体中通常分别是脯氨酸和羟脯氨酸。
Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid(or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
应用
鼠尾胶原蛋白的应用:
- 免疫组织化学
- 细胞活性检测
- 用于背根神经节(DRG)外植体的产生
- 作为官能化表面的制备组分之一(用于核磁共振)
- 用于细胞培养(玻璃盖玻片包被有高浓度的纳米线和胶原蛋白)
- 用于微通道的生物官能化
生化/生理作用
胶原蛋白是结缔组织的基本成分。一个中国家庭的研究表明COL1A1(胶原酶I型)突变与I型成骨不全有关。胶原蛋白和软骨下骨转换能力下调有关，可作为骨关节炎的关节间隙狭窄和骨刺状态的标志物。鼠尾胶原蛋白用于在组织培养板上铺上薄层包被，促进锚定依赖性细胞附着，推荐使用浓度为 6-10μg/cm2。本品不能用于制作3-D凝胶。I型胶原常作为细胞培养的附着基质，与成肌细胞、脊髓神经节、肝细胞、胚胎肺、心脏外植体、成纤维细胞、内皮细胞、胰岛细胞一起在I型胶原的膜或凝胶上成功培养。I型胶原也可用于特发性肺纤维化(IPF)的研究，研究内质网应激IPF对肺成纤维细胞的影响。酸性溶液中的胶原蛋白可以产生三维支架，用于生物工程和细胞培养。
制备说明:产品为澄清至浑浊的无色溶液，以1mg/ml 的浓度溶于含有2μl乙酸(或0.1N乙酸)的水中时存在少量不溶物。通过沉降或离心可去除此不溶物。
其他说明:胶原蛋白被分为许多种结构和遗传性不同的类型。我们使用Bornstein和Traub提出的命名法。不要混淆Sigma分类标识和公认的胶原蛋白分类类型。
同行评审论文(部分文献)
A neurite quality index and machine vision software for improved quantification of neurodegeneration.
Peggy Romero et al.
BioTechniques, 47(6 Suppl), iii-viii (2010-01-01)
Current methods to assess neurodegradation in dorsal root ganglion cultures as a model for neurodegenerative diseases are imprecise and time-consuming. Here we describe two new methods to quantify neuroprotection in these cultures. The neurite quality index (NQI) builds upon earlier
Regulation of vascular endothelial growth factor expression by extra domain B segment of fibronectin in endothelial cells.
Shali Chen et al.
Investigative ophthalmology & visual science, 53(13), 8333-8343 (2012-11-15)
Diabetic retinopathy entails proliferation of vascular endothelial cells (ECs) and unregulated angiogenesis. We have previously shown that ECs increase the expression of an embryonic variant of fibronectin (FN), called extra domain-B FN (ED-B FN) in response to high glucose. We
Circulating but not immobilized N-deglycosylated von Willebrand factor increases platelet adhesion under flow conditions.
M A Fallah et al.
Biomicrofluidics, 7(4), 44124-44124 (2014-01-10)
The role of von Willebrand factor (VWF) as a shear stress activated platelet adhesive has been related to a coiled-elongated shape conformation. The forces dominating this transition have been suggested to be controlled by the proteins polymeric architecture. However, the
A vitamin D analog ameliorates glomerular injury on rat glomerulonephritis.
K Makibayashi et al.
The American journal of pathology, 158(5), 1733-1741 (2001-05-05)
OCT (22-oxa-calcitriol), a vitamin D analog, has been reported to show strong inhibitory effects on mesangial cell proliferation in vitro. In the present study, we report a study of the effect of OCT on anti-thy-1 glomerulonephritis. Both OCT and 1,25(OH)(2)D(3)
High-resolution NMR imaging of paramagnetic liposomes targeted to a functionalized surface.
H P Huinink et al.
Magnetic resonance in medicine, 59(6), 1282-1286 (2008-04-19)
A major application of molecular MR imaging is receptor mapping of cells lining blood vessels with targeted contrast agents. Since these agents accumulate at interfaces, knowledge of their influence on the relaxation process in this specific configuration is a prerequisite
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文献和实验该产品被引用文献
Sigma货号C7661-5mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-10mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-25mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-50mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Sigma货号C7661-100mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
Rat Collagen Type I
from rat tail,powder,suitable for cell culture
说明
一般描述
所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成，其一级结构主要以三个氨基酸作为重复基序，即每隔两个氨基酸是一个甘氨酸残基，其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。I型胶原不同于其他胶原蛋白的方面为其低赖氨酸羟基化和低碳水化合物构成。Col1a1(胶原蛋白，I型，α1)或胶原蛋白是一种主要的人体结构蛋白，它以纤维的形式聚集。这是一种很长很薄的蛋白质，是人体中发现的最丰富的蛋白质。它是由三条左旋多肽链组成的超螺旋右螺旋结构。这些链由~1040个氨基酸组成，基本上是三个氨基酸-(Gly-X-Y)n的重复序列。Gly是甘氨酸，X和Y可以是任何氨基酸，但人体中通常分别是脯氨酸和羟脯氨酸。
Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid(or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
应用
鼠尾胶原蛋白的应用:
- 免疫组织化学
- 细胞活性检测
- 用于背根神经节(DRG)外植体的产生
- 作为官能化表面的制备组分之一(用于核磁共振)
- 用于细胞培养(玻璃盖玻片包被有高浓度的纳米线和胶原蛋白)
- 用于微通道的生物官能化
生化/生理作用
胶原蛋白是结缔组织的基本成分。一个中国家庭的研究表明COL1A1(胶原酶I型)突变与I型成骨不全有关。胶原蛋白和软骨下骨转换能力下调有关，可作为骨关节炎的关节间隙狭窄和骨刺状态的标志物。鼠尾胶原蛋白用于在组织培养板上铺上薄层包被，促进锚定依赖性细胞附着，推荐使用浓度为 6-10μg/cm2。本品不能用于制作3-D凝胶。I型胶原常作为细胞培养的附着基质，与成肌细胞、脊髓神经节、肝细胞、胚胎肺、心脏外植体、成纤维细胞、内皮细胞、胰岛细胞一起在I型胶原的膜或凝胶上成功培养。I型胶原也可用于特发性肺纤维化(IPF)的研究，研究内质网应激IPF对肺成纤维细胞的影响。酸性溶液中的胶原蛋白可以产生三维支架，用于生物工程和细胞培养。
制备说明:产品为澄清至浑浊的无色溶液，以1mg/ml 的浓度溶于含有2μl乙酸(或0.1N乙酸)的水中时存在少量不溶物。通过沉降或离心可去除此不溶物。
其他说明:胶原蛋白被分为许多种结构和遗传性不同的类型。我们使用Bornstein和Traub提出的命名法。不要混淆Sigma分类标识和公认的胶原蛋白分类类型。
同行评审论文(部分文献)
A neurite quality index and machine vision software for improved quantification of neurodegeneration.
Peggy Romero et al.
BioTechniques, 47(6 Suppl), iii-viii (2010-01-01)
Current methods to assess neurodegradation in dorsal root ganglion cultures as a model for neurodegenerative diseases are imprecise and time-consuming. Here we describe two new methods to quantify neuroprotection in these cultures. The neurite quality index (NQI) builds upon earlier
Regulation of vascular endothelial growth factor expression by extra domain B segment of fibronectin in endothelial cells.
Shali Chen et al.
Investigative ophthalmology & visual science, 53(13), 8333-8343 (2012-11-15)
Diabetic retinopathy entails proliferation of vascular endothelial cells (ECs) and unregulated angiogenesis. We have previously shown that ECs increase the expression of an embryonic variant of fibronectin (FN), called extra domain-B FN (ED-B FN) in response to high glucose. We
Circulating but not immobilized N-deglycosylated von Willebrand factor increases platelet adhesion under flow conditions.
M A Fallah et al.
Biomicrofluidics, 7(4), 44124-44124 (2014-01-10)
The role of von Willebrand factor (VWF) as a shear stress activated platelet adhesive has been related to a coiled-elongated shape conformation. The forces dominating this transition have been suggested to be controlled by the proteins polymeric architecture. However, the
A vitamin D analog ameliorates glomerular injury on rat glomerulonephritis.
K Makibayashi et al.
The American journal of pathology, 158(5), 1733-1741 (2001-05-05)
OCT (22-oxa-calcitriol), a vitamin D analog, has been reported to show strong inhibitory effects on mesangial cell proliferation in vitro. In the present study, we report a study of the effect of OCT on anti-thy-1 glomerulonephritis. Both OCT and 1,25(OH)(2)D(3)
High-resolution NMR imaging of paramagnetic liposomes targeted to a functionalized surface.
H P Huinink et al.
Magnetic resonance in medicine, 59(6), 1282-1286 (2008-04-19)
A major application of molecular MR imaging is receptor mapping of cells lining blood vessels with targeted contrast agents. Since these agents accumulate at interfaces, knowledge of their influence on the relaxation process in this specific configuration is a prerequisite
相关实验
primary fibroblasts (˜ cell number 1 x 106 ) use: A. 25 ml rat tail collagen (approx conc 2mg/ml) B. 3 ml 10xMEM C. 0.22 M NaOH: first, add 2ml, then drop-wise whilst stirring, until the collagen turns
来源的血管类器官培养方案【3】 细胞来源 hPSCs 培养试剂配方 hPSCs 培养基 中胚层分化培养基 中胚层分化培养基-2 StemPro-34 SFM 完全培养基 Collagen I 溶液 血管分化培养基 近岸蛋白产品货号 基础培养基 DMEM-F12 50％DMEM-F12 50％DMEM-F12 StemPro-34 SFM DMEM-F12 StemPro-34 SFM - 50％
Direct tissue explant method for derivation and study of extravillous trophoblast
the explant 3x with PBS. Remove the villous tissue from the collagen with fine forceps and incubate the remaining cells with 100 mg/ml collagenase (Sigma type 1A) at 37°C. Collagenase should be reconstituted in DEPC-treated sterile PBS if the isolated
文献支持
Sigma货号C7661-100mg胶原蛋白I型(来源于大鼠尾)(Rat Collagen Type I)上海睿安生物13611631389
￥8475
