γS-crystallin蛋白抗体

GTPγS-induced Inhibition of Binding

GTPγS-induced Inhibition of Binding 1. Chemicals and Equipment DB Buffer, PB, cAMP, cAMP [2, 8- 3H] (25 Ci/mmol, 1.0 mCi/ml, 32, 000 nM), 10 mM GTPγS, Wacker silicone AR-20, Reagiergefab test tubes (Sarstedt), millipore

Measurement of Agonist-Stimulated [35S]GTPγS Binding to Assess Total G-Protein and Gα-Subtype-Specific Activation by G-Protein-Coupled Receptors

preparations, GTP can be substituted by 35 S-labeled guanosine 5′-O -(3-thio)triphosphate ([35 S]GTγS) and on agonist stimulation a stable [35 S]GTPγS-Gα complex will form and accumulate. Separation of 35 S-bound GTPγS-Gα complexes from free [35 S]GTPγS allows

【资源】常见蛋白质等电点参考值

蛋白[myogen A] 6.3 β-乳球蛋白[β-lactoglobulin] 5.1－5.3 卵黄蛋白[livetin] 4.8－5.0 γ1—球蛋白（人）[γ1-globulin(human)] 5.8，6.6，7.3，8.2 γ2—球蛋白（人）[γ2-globulin(human)] 5.2－5.5 肌球蛋白A[myosin A] 5.1 原肌球蛋白[myosin A] 5.9 铁传递蛋白[siderophilin] 3.4－3.5 胎球蛋白[fetuin] 5.5－5.8 血纤蛋白