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现货
- 供应商：
上海善然生物科技有限公司
grade Vetec™ reagent grade
assay 98%
form powder
mp 195 °C (dec.)(lit.)
suitability suitable for
storage temp. 2-8°C
Description
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical tochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
Legal Information
Vetec is a trademark of Sigma-Aldrich International GmbH
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文献和实验Tetrazolium (MTT) Assay for Cellular Viability and Activity
assays utilizes 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), a water-soluble yellow dye that is readily taken up by viable cells and reduced by the action of mitochondrial dehydrogenases ( 1 ). The reduction product is a water
by Percoll gradient separation. Purity is assessed by flow cytometry using staining against cytokeratin-7, protein specific for trophoblast cells. Cell proliferation is evaluated by a Thiazolyl Blue Tetrazolium Bromide (MTT) assay, hormonal secretion
MTT Growth Assays in Ovarian Cancer
The MTT (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide) growth assay developed by Mosmann (1 ) offers a simple, rapid, and precise measurement of cell viability and proliferation of adherent cell lines (2 ). The value
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