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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Anti-Phosphotyrosine Antibody, clone 4G10®
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
rade name Upstate
brand family Upstate
format Purified
control Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
molecular weight Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
application(s) Immunocytochemistry
Immunohistochemistry
展开 (21)
brand family Upstate
format Purified
control Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
molecular weight Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
application(s) Immunocytochemistry
Immunohistochemistry
展开 (21)
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文献和实验相关实验
≥1 μl (as required) Platinum® Taq Antibody: Taq DNA polymerase 1 μl 2.5 units (or as required
a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
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Anti-Phosphotyrosine Antibody, clone 4G10®
¥3112
