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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Nucleophosmin (NPM)
- 库存：
100
- 供应商：
上海沪震
核仁磷酸蛋白(NPM)重组蛋白 Recombinant Nucleophosmin (NPM)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
核仁磷酸蛋白(NPM)重组蛋白
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 11.3kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
核仁磷酸蛋白(NPM)重组蛋白
Subcellular Location n/a
Residues Ala204~Trp290 (Accession # P06748) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- AQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
核仁磷酸蛋白(NPM)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-2222R cellulase纤维素酶抗体
hz-8541R CLEC 4EC型凝集素4家族E抗体
hz-0236R ChRM2毒蕈碱型乙酰胆碱受体抗体
hz-0441R ChRM2毒蕈碱型乙酰胆碱受体M2抗体
hz-0544R CIDEB促进凋亡基因抗体
hz-0670R C-jun原癌基因蛋白/活化蛋白1抗体
hz-6796R CIDEC细胞死亡活化蛋白抗体
hz-1028R Cytokeratin 19细胞角蛋白19抗体
hz-1005R CK2细胞角蛋白2抗体
hz-1006R CK4细胞角蛋白4抗体
hz-3879R Cytochrome b245 Light Chain细胞色素b245轻链抗体
hz-15205R C5ORF445号染色体开放阅读框44抗体
hz-7685R Corin心脏特异性丝氨酸蛋白酶抗体
hz-7560R CPLA2胞浆型磷脂酶A2抗体
hz-7064R CCAR1细胞周期及凋亡调节蛋白1抗体
hz-0805R CD56神经细胞粘附分子1抗体
hz-1241R Claudin 5紧密连接蛋白5抗体
hz-2034R CRIM1富含半胱氨酸运动神经元蛋白1抗体
hz-1660R CK5+6细胞角蛋白5+6抗体
hz-0842R c-Myc tagc-Myc tag标签抗体
hz-0848R CSP环子孢子蛋白(约氏疟原虫)抗体
hz-15172R C3orf30 3号染色体开放阅读框30抗体
hz-0811R CX3CL1CX3CL1抗体
hz-0488R CXorf36脱羧酶蛋白体36抗体
hz-15183R C3orf783号染色体开放阅读框78抗体
hz-0324R C5a anaphylatoxin过敏毒素C5a/补体C5a抗体
hz-0091R CA125卵巢癌抗原抗体
hz-1685R CTNNAL1粘附分子相关蛋白a1抗体
hz-15127R C21orf6921号染色体开放阅读框69抗体
hz-1780R CCRK细胞周期相关激酶抗体
hz-1239R MUC1乳腺癌相关抗原抗体
hz-0084R Caspase-6 (NT)半胱胺酸蛋白酶蛋白-6抗体(N端)
hz-1680R CDK3周期素依赖性激酶3抗体
hz-0030M Calcineurin A钙调磷酸酶抗体
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
核仁磷酸蛋白(NPM)重组蛋白
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 11.3kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
核仁磷酸蛋白(NPM)重组蛋白
Subcellular Location n/a
Residues Ala204~Trp290 (Accession # P06748) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- AQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
核仁磷酸蛋白(NPM)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-2222R cellulase纤维素酶抗体
hz-8541R CLEC 4EC型凝集素4家族E抗体
hz-0236R ChRM2毒蕈碱型乙酰胆碱受体抗体
hz-0441R ChRM2毒蕈碱型乙酰胆碱受体M2抗体
hz-0544R CIDEB促进凋亡基因抗体
hz-0670R C-jun原癌基因蛋白/活化蛋白1抗体
hz-6796R CIDEC细胞死亡活化蛋白抗体
hz-1028R Cytokeratin 19细胞角蛋白19抗体
hz-1005R CK2细胞角蛋白2抗体
hz-1006R CK4细胞角蛋白4抗体
hz-3879R Cytochrome b245 Light Chain细胞色素b245轻链抗体
hz-15205R C5ORF445号染色体开放阅读框44抗体
hz-7685R Corin心脏特异性丝氨酸蛋白酶抗体
hz-7560R CPLA2胞浆型磷脂酶A2抗体
hz-7064R CCAR1细胞周期及凋亡调节蛋白1抗体
hz-0805R CD56神经细胞粘附分子1抗体
hz-1241R Claudin 5紧密连接蛋白5抗体
hz-2034R CRIM1富含半胱氨酸运动神经元蛋白1抗体
hz-1660R CK5+6细胞角蛋白5+6抗体
hz-0842R c-Myc tagc-Myc tag标签抗体
hz-0848R CSP环子孢子蛋白(约氏疟原虫)抗体
hz-15172R C3orf30 3号染色体开放阅读框30抗体
hz-0811R CX3CL1CX3CL1抗体
hz-0488R CXorf36脱羧酶蛋白体36抗体
hz-15183R C3orf783号染色体开放阅读框78抗体
hz-0324R C5a anaphylatoxin过敏毒素C5a/补体C5a抗体
hz-0091R CA125卵巢癌抗原抗体
hz-1685R CTNNAL1粘附分子相关蛋白a1抗体
hz-15127R C21orf6921号染色体开放阅读框69抗体
hz-1780R CCRK细胞周期相关激酶抗体
hz-1239R MUC1乳腺癌相关抗原抗体
hz-0084R Caspase-6 (NT)半胱胺酸蛋白酶蛋白-6抗体(N端)
hz-1680R CDK3周期素依赖性激酶3抗体
hz-0030M Calcineurin A钙调磷酸酶抗体
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文献和实验相关实验
farnesylcysteine|法尼半胱氨酸 farnesylpyrophosphate|法尼焦磷酸，焦磷酸法尼酯 Farr technique|Farr技术，法尔技术[测定抗体绝对量的放射免疫技术] Farwestern blotting|Farwestern印迹法，蛋白质检测（的）蛋白质印迹法[例如用标记的激酶或重组蛋白 来检测印迹膜上与之作用的蛋白] fasciclin|成束蛋白[参与轴突的成束]
、不损伤细胞，尽量在液体和生理条件下操作； 所用试剂和膜材料对细胞无毒害； 膜的孔径可控制，必须使营养物和代谢物自由通过； 膜应有足够机械强度抵抗培养中搅拌。 五、抗凋亡策略在细胞大规模培养中的应用 生物反应器动物细胞大规模生产过程中，细胞凋亡在细胞死亡中占主要部分。最近研究显示在大规模培养生物反应器中细胞的死亡中80%是凋亡所导致,而不是以前所认为的坏死。而在大规模细胞培养中，细胞死亡是维持细胞高活性和高密度的最大障碍。理论上讲，防止或延长细胞死亡，可以极大提高生物反应器生产重组蛋白
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核仁磷酸蛋白(NPM)重组蛋白
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