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- ZYMO RESEARCH
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- 2026年02月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
ZR BAC DNA Miniprep Kit
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
常温
HIGHLIGHTS
- High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
- Unique column design: Zero buffer retention and low elution volume (10 µl)
DESCRIPTION
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
| • | For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion, etc. No messy precipitations! |
| • | Innovative colored buffers* for rapid identification of complete bacterial cell lysis and neutralization steps. |
| • | Unique column design: zero buffer retention and low-volume (≥10 μl) elution. |
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文献和实验freeshotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
free shotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet were resuspended in 265 μl TE
