ZR BAC DNA Miniprep Kit

HIGHLIGHTS

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• High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
• Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
• Unique column design: Zero buffer retention and low elution volume (10 µl)

DESCRIPTION

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The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.

•	For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion, etc. No messy precipitations!
•	Innovative colored buffers* for rapid identification of complete bacterial cell lysis and neutralization steps.
•	Unique column design: zero buffer retention and low-volume (≥10 μl) elution.