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- U1300
- 2026年05月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
150u
| U1300 | DNA Polymerase I Large (Klenow) Fragment Mini Kit | 150u | 2,391 |
| U1330 | Set of dATP, dCTP, dGTP, dTTP | 10µmol each | 762 |
| U1335 | Set of dATP, dCTP, dGTP, dUTP | 10µmol each | 762 |
| U1410 | Set of dATP, dCTP, dGTP, dTTP | 200µmol(2 × 100µmol each) | 9,759 |
| U1420 | Set of dATP, dCTP, dGTP, dTTP | 25µmol each | 1,720 |
| U1511 | dNTP Mix | 200µl | 178 |
| U1515 | dNTP Mix | 1,000µl | 461 |
| U2010 | DNA 5' End-Labeling System | 10 reactions | 2,123 |
| V1061 | Chymotrypsin, Sequencing Grade | 25µg | 1,039 |
| V1062 | Chymotrypsin, Sequencing Grade | 4 x 25µg | 3,115 |
| V1071 | Endoproteinase Lys-C, Sequencing Grade | 5µg | 2,012 |
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文献和实验Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3′–5′ exonuclease activity, but lacks the 5′–3′ exonuclease activity
Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1
Historically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(1 . This enzyme was derived from E. coli . DNA Pol I which, in addition to polymerase activity, has both 3′–
), where we have overcome both the size limitations and the restrictions of the cloning/subcloning procedures used in the Red/ET recombineering system. We describe the transfer of a very large DNA fragment (~55 kb) from one BAC into another unrelated BAC by the means
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