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文献和实验ULTIMATE FREEZE-THAW LYSIS FOR MAMMALIAN CELLS
-abundance proteins (like Myc!).The protocol is described for harvesting mammalian cells from 6 or 10 cm tissue-culture dishes.A.CELL HARVEST.1.Remove media from cells.Wash in ice-cold PBS.2.Scrape cells in 1 ml of ice-cold PBS.Transfer to 1.5 ml microfuge
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison. The ups and downs of various methods from sonication, homogenization, freeze-thaw cycles
Cell Lysis/Western/IPSteven Finkbeiner,Departments of Neurology and Physiology,UCSFcompiled by 6/95 by AJS from AB)Fresh 1X LysisFresh 1X LysisFinal5ml10ml2X Lysis 2.5 ml5mlNaCl0.25M0.25ml of 5M0.5 ml of 5MNa3VO40.1mM25l (μl) of 200 mM5l (μl) of 200
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