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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
1 × 96 preps
| MD1370 | MagneSil® ONE, Fixed Yield Blood Genomic System | 1 × 96 preps | 2,278 |
| MD1392 | Lysis Buffer, Blood | 160ml | 850 |
| MD1401 | Salt Wash, Blood | 90ml | 751 |
| MD1411 | Alcohol Wash, Blood | 70ml | 148 |
| MD1412 | Alcohol Wash, Blood | 120ml | 288 |
| MD1421 | Elution Buffer, Blood | 45ml | 110 |
| MD1431 | Anti-Foam Reagent | 300µl | 238 |
| MD1441 | MagneSil® Paramagnetic Particles | 25ml | 1,140 |
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文献和实验Automated Genomic DNA Extraction
实验材料 10-20 µl blood samples 实验步骤 Before Starting Perform the following before beginning: Prepare Lysis Mix: For each sample, mix 0.5 ml of ChargeSwitch® Lysis Buffer (L12) and 5 µl of Proteinase K
Standard Protocol For Isolation of DNA From Dried Blood, Body Fluids and Sperm
system allows genomic DNA up to 50 kb to bind to the matrix. Samples are first lysed under denaturing conditions and then applied to the HiBind® spin columns to which the DNA binds, while cellular debris, hemoglobin, and other proteins are effectively washed
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
extension cycle at 72 ˚C for 5 min. PCR reactions were performed in Gene Amp® PCR System 480 (Perkin-Elmer, Norwalk, CT, USA). DNA samples of normal subjects (blood donors) were included as well as negative control (with no DNA sample) in each reaction.
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