- ¥857
- promega
- 进口
- L1001
- 2026年01月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
1ml
| L1001 | JM109 Competent Cells, >107cfu/µg | 1ml | 857 | ||
| L1020 | E. coli S30 Extract System for Circular DNA | 30 reactions | 3,667 | ||
| L1030 | E. coli S30 Extract System for Linear Templates | 30 reactions | 3,667 | ||
| L1061 | pF25A ICE T7 Flexi® Vector | 20µg | 2,538 | ||
| L1081 | pF25K ICE T7 Flexi® Vector | 20µg | 2,538 | ||
| L1101 | TNT® T7 Insect Cell Extract Protein Expression System | 10 reactions | 2,044 | ||
| L1102 | TNT® T7 Insect Cell Extract Protein Expression System | 40 reactions | 7,155 | ||
| L1110 | S30 T7 High-Yield Protein Expression System | 24 reactions | 5,178 | ||
| L1115 | S30 T7 High-Yield Protein Expression System | 8 reactions | 2,044 | ||
| L1130 | E. coli T7 S30 Extract System for Circular DNA | 30 reactions | 3,868 |
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文献和实验Step 1: Take an 100 mL aliquot of frozen cells from the -80oC and innoculate about 500 ml to 1 L sterile LB broth. DO NOT ADD antibiotic
Preparation of Lactobacillus Competent Cells
Overview Instructions on how to prepare Lactobacillus plantarum competent cells before electrotransformation. Materials MRS media Culture of L. plantarum cells MgCL2 (10mM) sucrose glycerol
Transforming chemically competent cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). Note: If you are adding small volumes (~1μl), be careful to mix the culture well. Diluting the plasmid
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