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- promega
- 进口
- L2001
- 2026年01月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
1ml
| L2001 | JM109 Competent Cells, >108cfu/µg | 1ml | 1,151 |
| L2005 | Single-use JM109 Competent Cells, >108cfu/µg | 1ml (20 × 50µl) | 2,026 |
| L2011 | HB101 Competent Cells, >108cfu/µg | 1ml | 990 |
| L2015 | Single-use HB101 Competent Cells | 1ml (20 × 50µl) | 1,771 |
| L2080 | TNT® SP6 Quick Coupled Transcription/Translation System | 40 reactions | 4,819 |
| L2081 | TNT® SP6 Quick Coupled Transcription/Translation System, Trial Size | 5 reactions | 1,320 |
| L3002 | Single Step (KRX) Competent Cells | 20 × 50µl | 2,501 |
| L3260 | TNT® SP6 High-Yield Protein Expression System | 40 reactions | 5,446 |
| L3261 | TNT® SP6 High-Yield Protein Expression System | 10 reactions | 1,694 |
| L4130 | TNT® SP6 Coupled Wheat Germ Extract System | 40 reactions | 4,433 |
| L4140 | TNT® T7 Coupled Wheat Germ Extract System | 40 reactions | 4,433 |
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文献和实验TOP10 chemically competent cells
should yield around 100 - 400 colonies Transformation efficiency is (dilution factor=15) x colony count x 105 /µgDNA We expect that the transformation efficiency should be between 5x108 and 5x109 cfu/µgDNA 5x Ligation Adjustment
Step 1: Take an 100 mL aliquot of frozen cells from the -80oC and innoculate about 500 ml to 1 L sterile LB broth. DO NOT ADD antibiotic
Preparation of Lactobacillus Competent Cells
Overview Instructions on how to prepare Lactobacillus plantarum competent cells before electrotransformation. Materials MRS media Culture of L. plantarum cells MgCL2 (10mM) sucrose glycerol
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