Bridging Integrator 1 (BIN1) 抗

Bridging Integrator 1 (BIN1) 抗体抗原
Bridging Integrator 1 (BIN1)
别名 bin1, MGC53185, amph2, amphl, sh3p9, MGC76187, cb57, zgc:86701, BIN1, AMPH2, AMPHL, SH3P9, ALP-1, Amphl, BRAMP-2
适用
其他选择 人
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (12A)
标记
其他选择 非结合性
应用范围
其他选择 Western Blotting (WB), 酶联免疫吸附测定（ELISA）
Pubmed 找到1个引用
规格 25 μL
发货至 中国 (更改)
产品编号 yb1043790
产品细节 Bridging Integrator 1 (BIN1) 抗体
免疫原 Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with 12A exon BIN1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein
克隆位点 12A
亚型 IgG
特异性 Anti-BIN1 purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.
产品特性 Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act with cancer suppressor and inhibits malignant cell transformation. A Study in human tumor cell lines found that most melanoma cells inappropriately expressed exon 12A, suggests that the aberrant splicing of Bin1 may contribute to melanoma progression.
纯化方法 Antiserum
过滤 Sterile filtered
目标详细情况 Bridging Integrator 1 (BIN1) 抗体
别名 BIN1
背景 Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act with cancer suppressor and inhibits malignant cell transformation. A Study in human tumor cell lines found that most melanoma cells inappropriately expressed exon 12A, suggests that the aberrant splicing of Bin1 may contribute to melanoma progression.
Synonyms: AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN 1, BIN-1, BIN1 antibody, anti-BIN1 antibody
基因ID 274
NCBI登录号 NP_004296
UniProt O00499
研究领域 Cancer, Cell Cycle, Transcription Factors
使用细节 Bridging Integrator 1 (BIN1) 抗体
应用备注 Anti-BIN1 antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.
说明
Gene Name: BIN1
限制 仅限研究用
贮存及处理 Bridging Integrator 1 (BIN1) 抗体
状态 Liquid
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
有效期 12 months