- ￥415 - 3977
- Invent
- 美国
- CP-011
- 2026年04月22日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Minute™ Chloroplast Isolation Kit
- 规格：
50 Preps/4 Preps
| 规格： | 50 Preps | 产品价格： | ￥3977.0 |
|---|---|---|---|
| 规格： | 4 Preps | 产品价格： | ￥415.0 |
说明：叶绿体分离试剂盒中包含优化的叶绿体提取缓冲液和2.0ml离心管柱。利用带孔径的离心管柱技术可以快速从新鲜的植物组织中（叶片，种子，软茎等）提取叶绿素。其他方法都需要1-10g组织来提取叶绿体，我们的试剂盒可以从100-200mg新鲜植物叶片中快速提取1 X 106 - 1 X 107 的完整叶绿体（>90%完整），操作时间<5分钟。
特点：
- 简单，快速，性价比高
- 得率高
- 小样品量，可从小样本中提取叶绿体的商业试剂盒
- 适用于筛选或下游应用（如叶绿体DNA提取）
- 储存条件：buffer A and B储存于-20℃
参考文献：
1. H Oh et al. (2015) Two complete chloroplast genome sequences of Cannabis sativa varieties Mitochondrial DNA. DOI:10.3109/19401736.2015.1053117.
2. Hu, D., Li, Y., Jin, W., Gong, H., He, Q., & Li, Y. (2017). Identification and Characterization of a Plastidic Adenine Nucleotide Uniporter (OsBT1-3) Required for Chloroplast Development in the Early Leaf Stage of Rice. Scientific RepoRts | 7:41355 | DOI: 10.1038/srep41355.
3. Kanazawa, A., Ostendorf, E., Kohzuma, K., Hoh, D., Strand, D. D., Sato-Cruz, M., ... & Kramer, D. M. (2017).Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force:Implications for Photosystem I and Photosystem II Photoprotection. Front. Plant Sci., 03 May 2017 | DOI: org/10.3389/fpls.2017.00719
4. Suzuki, J.Y., Geib, S.M., Carlsen, M.M., Henriquez, C.L., Amore, T.D., Sim, S.B., Matsumoto, T.K., Keith, L.M. and Myers, R.Y. (2017). Development of chloroplast single nucleotide polymorphisms (SNPs) as a tool towards interspecies typing of Anthurium germplasm. Acta Hortic. 1167, 257-270
5. H ShuoHao etal (2018).Identification and characterization of a pyridoxal 5′-phosphate phosphatase in tobacco plants. Plant science.doi.org/10.1016/j.gene.2018.10.078.
6. Fuli Ma etal (2019).Dynamic translocation of stilbene synthase VpSTS29 from a Chinese wild Vitis species upon UV irradiation.Phytochemistry.doi.org/10.1016/j.phytochem.2018.12.019.
7. Ren, R. C., Wang, L. L., Zhang, L., Zhao, Y. J., Wu, J. W., Wei, Y. M., ... & Zhao, X. Y. DEK43 is a P‐type PPR Protein Responsible for the Cis‐splicing of nad4 in Maize Mitochondria. Journal of Integrative Plant Biology.doi: 10.1111/jipb.12843.
8. Lee, S., Joung, Y. H., Kim, J. K., Do Choi, Y., & Jang, G. (2019). An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development. BMC Plant Biology, 19(1), 524.
9. Yin, B., Zhang, J., Liu, Y., Pan, X., Zhao, Z., Li, H., ... & Liu, D. (2019). PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr. Scientific Reports, 9(1), 1-15.
10. Wang chengliang et al(2020).Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice.NEW PHYTOLOGIST.
11. Shoeva, O. Y., Mursalimov, S. R., Gracheva, N. V., Glagoleva, A. Y., Börner, A., & Khlestkina, E. K. (2020). Melanin formation in barley grain occurs within plastids of pericarp and husk cells. Scientific Reports, 10(1), 1-9
12.Long, J., Xing, W., Wang, Y., Wu, Z., Li, W., Zou, Y., ... & Zhang, F. (2021). Comparative Proteomic Analysis on Chloroplast Proteins Provides New Insights Into the Effects of Low Temperature in Sugar Beet.
13.Shapiro, K. O. (2021). Plug and play: Is “directed endosymbiosis” of chloroplasts possible?. bioRxiv.
14.Pan, T., Liu, Y., Ling, C., Tang, Y., Tang, W., Liu, Y., ... & Wang, S. (2021). CV-containing vesicle budding from chloroplast inner envelope membrane is mediated by clathrin but inhibited by GAPC. bioRxiv.
15.Li, C., Li, J., Du, X., Zhang, J., Zou, Y., Liu, Y., ... & Lu, H. (2022). Chloroplast Thylakoidal Ascorbate Peroxidase, PtotAPX, Has Enhanced Resistance to Oxidative Stress in Populus tomentosa. International Journal of Molecular Sciences, 23(6), 3340.
16.Shi-Kai Cao, Rui Liu, Miaodi Wang, Feng Sun, Aqib Sayyed, Hong Shi, Xiaomin Wang, Bao-Cai Tan, The small PPR protein SPR2 interacts with PPR-SMR1 to facilitate the splicing of introns in maize mitochondria, Plant Physiology, 2022;, kiac379, doi.org/10.1093/plphys/kiac379
17.Belanger, S., Kramer, M. C., Payne, H. A., Slotkin, R. K., Meyers, B. C., & Staub, J. M. (2022). Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genes. bioRxiv.
18.Liu, L., He, X., Wang, S., Qin, X., Che, S., Wu, L., ... & Yang, M. (2023). The role of the chloroplast localised phosphate transporter GmPHT4; 10 gene in plant growth, photosynthesis and drought resistance. Functional Plant Biology.
19.Wang, Y. Q., Ye, J. J., Yang, H. Z., Li, D., Li, X. X., Wang, Y. K., ... & Lu, J. L. (2023). Shading-Dependent Greening Process of the Leaves in the Light-Sensitive Albino Tea Plant ‘Huangjinya’: Possible Involvement of the Light-Harvesting Complex II Subunit of Photosystem II in the Phenotypic Characteristic. International Journal of Molecular Sciences, 24(12), 10314.
20.Nworji, O., Ogana, J., Orji, E., Ezeigwe, O., & Enemor, A. (2023). The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis.
21.Pan, T., Liu, Y., Hu, X., Li, P., Lin, C., Tang, Y., ... & Wang, S. (2023). Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC. Cell Reports, 42(10).
22.The, S. V., Santiago, J. P., Pappenberger, C., Hammes, U. Z., & Tegeder, M. (2023). UMAMIT44 is a key player in glutamate export from Arabidopsis chloroplasts. The Plant Cell, koad310.
23.Wang, X., Yang, J., Hu, H., Yuan, T., Zhao, Y., Liu, Y., ... & Liu, J. (2024). Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana. Viruses, 16(4), 489.
24.Sun, X., Singla-Rastogi, M., Wang, J., Zhao, C., Wang, X., & Li, P. (2024). The uS10c-BPG2 module mediates ribosomal RNA processing in chloroplast nucleoids. Nucleic Acids Research, gkae339.
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- 作者
- 内容
- 询问日期
文献和实验1. H Oh et al. (2015) Two complete chloroplast genome sequences of Cannabis sativa varieties Mitochondrial DNA. DOI:10.3109/19401736.2015.1053117.
2. Hu, D., Li, Y., Jin, W., Gong, H., He, Q., & Li, Y. (2017). Identification and Characterization of a Plastidic Adenine Nucleotide Uniporter (OsBT1-3) Required for Chloroplast Development in the Early Leaf Stage of Rice. Scientific RepoRts | 7:41355 | DOI: 10.1038/srep41355.
3. Kanazawa, A., Ostendorf, E., Kohzuma, K., Hoh, D., Strand, D. D., Sato-Cruz, M., ... & Kramer, D. M. (2017).Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force:Implications for Photosystem I and Photosystem II Photoprotection. Front. Plant Sci., 03 May 2017 | DOI: org/10.3389/fpls.2017.00719
4. Suzuki, J.Y., Geib, S.M., Carlsen, M.M., Henriquez, C.L., Amore, T.D., Sim, S.B., Matsumoto, T.K., Keith, L.M. and Myers, R.Y. (2017). Development of chloroplast single nucleotide polymorphisms (SNPs) as a tool towards interspecies typing of Anthurium germplasm. Acta Hortic. 1167, 257-270
5. H ShuoHao etal (2018).Identification and characterization of a pyridoxal 5′-phosphate phosphatase in tobacco plants. Plant science.doi.org/10.1016/j.gene.2018.10.078.
6. Fuli Ma etal (2019).Dynamic translocation of stilbene synthase VpSTS29 from a Chinese wild Vitis species upon UV irradiation.Phytochemistry.doi.org/10.1016/j.phytochem.2018.12.019.
7. Ren, R. C., Wang, L. L., Zhang, L., Zhao, Y. J., Wu, J. W., Wei, Y. M., ... & Zhao, X. Y. DEK43 is a P‐type PPR Protein Responsible for the Cis‐splicing of nad4 in Maize Mitochondria. Journal of Integrative Plant Biology.doi: 10.1111/jipb.12843.
8. Lee, S., Joung, Y. H., Kim, J. K., Do Choi, Y., & Jang, G. (2019). An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development. BMC Plant Biology, 19(1), 524.
9. Yin, B., Zhang, J., Liu, Y., Pan, X., Zhao, Z., Li, H., ... & Liu, D. (2019). PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr. Scientific Reports, 9(1), 1-15.
10. Wang chengliang et al(2020).Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice.NEW PHYTOLOGIST.
11. Shoeva, O. Y., Mursalimov, S. R., Gracheva, N. V., Glagoleva, A. Y., Börner, A., & Khlestkina, E. K. (2020). Melanin formation in barley grain occurs within plastids of pericarp and husk cells. Scientific Reports, 10(1), 1-9
12.Long, J., Xing, W., Wang, Y., Wu, Z., Li, W., Zou, Y., ... & Zhang, F. (2021). Comparative Proteomic Analysis on Chloroplast Proteins Provides New Insights Into the Effects of Low Temperature in Sugar Beet.
13.Shapiro, K. O. (2021). Plug and play: Is “directed endosymbiosis” of chloroplasts possible?. bioRxiv.
14.Pan, T., Liu, Y., Ling, C., Tang, Y., Tang, W., Liu, Y., ... & Wang, S. (2021). CV-containing vesicle budding from chloroplast inner envelope membrane is mediated by clathrin but inhibited by GAPC. bioRxiv.
15.Li, C., Li, J., Du, X., Zhang, J., Zou, Y., Liu, Y., ... & Lu, H. (2022). Chloroplast Thylakoidal Ascorbate Peroxidase, PtotAPX, Has Enhanced Resistance to Oxidative Stress in Populus tomentosa. International Journal of Molecular Sciences, 23(6), 3340.
16.Shi-Kai Cao, Rui Liu, Miaodi Wang, Feng Sun, Aqib Sayyed, Hong Shi, Xiaomin Wang, Bao-Cai Tan, The small PPR protein SPR2 interacts with PPR-SMR1 to facilitate the splicing of introns in maize mitochondria, Plant Physiology, 2022;, kiac379, doi.org/10.1093/plphys/kiac379
17.Belanger, S., Kramer, M. C., Payne, H. A., Slotkin, R. K., Meyers, B. C., & Staub, J. M. (2022). Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genes. bioRxiv.
18.Liu, L., He, X., Wang, S., Qin, X., Che, S., Wu, L., ... & Yang, M. (2023). The role of the chloroplast localised phosphate transporter GmPHT4; 10 gene in plant growth, photosynthesis and drought resistance. Functional Plant Biology.
19.Wang, Y. Q., Ye, J. J., Yang, H. Z., Li, D., Li, X. X., Wang, Y. K., ... & Lu, J. L. (2023). Shading-Dependent Greening Process of the Leaves in the Light-Sensitive Albino Tea Plant ‘Huangjinya’: Possible Involvement of the Light-Harvesting Complex II Subunit of Photosystem II in the Phenotypic Characteristic. International Journal of Molecular Sciences, 24(12), 10314.
20.Nworji, O., Ogana, J., Orji, E., Ezeigwe, O., & Enemor, A. (2023). The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis.
21.Pan, T., Liu, Y., Hu, X., Li, P., Lin, C., Tang, Y., ... & Wang, S. (2023). Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC. Cell Reports, 42(10).
22.The, S. V., Santiago, J. P., Pappenberger, C., Hammes, U. Z., & Tegeder, M. (2023). UMAMIT44 is a key player in glutamate export from Arabidopsis chloroplasts. The Plant Cell, koad310.
23.Wang, X., Yang, J., Hu, H., Yuan, T., Zhao, Y., Liu, Y., ... & Liu, J. (2024). Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana. Viruses, 16(4), 489.
24.Sun, X., Singla-Rastogi, M., Wang, J., Zhao, C., Wang, X., & Li, P. (2024). The uS10c-BPG2 module mediates ribosomal RNA processing in chloroplast nucleoids. Nucleic Acids Research, gkae339.
25. Yong-Bo Lv; Dashuai Zhu; Junlang Li; Yuan Li; Chao Lü; et al.(2025).Plant-derived hydrogel and photosynthetic nano-units for myocardial infarction therapy.Nature Communications.DOI 10.1038/s41467-025-62020-5
26.Franziska Kuhnert; Philipp Westhoff; Velasco Valencia; Stephan Krüger; Katharina Vogel; et al.(2025).RETICULATA1 is a plastid-localized basic amino acid transporter.Nature Plants.DOI 10.1038/s41477-025-02080-z
27.Sunghwan Bae; Hyoseong Ahn; Minha Kim; Sangun Park; Yongduk Kim.(2025). Tailored Spectral Lighting Enhances Growth and Photosynthetic Efficiency of Wasabia japonica. Horticulturae.DOI 10.3390/horticulturae11020162
、叶绿体、有环片层和间体等；属于颗粒性结构的有核糖体（或者多核糖体）、糖原颗粒、色素粒、脂肪滴和结晶体等；另外，属于纤维性结构的有微管、中心体、纤毛、鞭毛和微丝。而在这些结构之间充满着液态的细胞质基质。在电子显微镜下观察，细胞质基质是均质的，无结构的，但是，其中含有许多酶和其他蛋白质。细胞质的物理特性表现于细胞质基质的性质。细胞质的粘性是 5— 50里泊（ cp），但在物理性上显示出在纯粘性流体中所见不到的弹性、曳丝性和溶胶与凝胶的转化性（触变性）。而且，细胞质在光学上通常呈等方性，但是，在太阳虫类
柱式法 Minute TM 胞质胞核分离试剂盒操作方法：A． 悬浮细胞样品（包括植物，细菌，酵母和真菌制备的原生质体）1.500Xg，3 分钟低速离心收集细胞，用预冷的 PBS 清洗一次2. 将细胞转移到 1.5 ml 离心管中，3000 rpm 离心 1-5 分钟，弃去上清。3. 按表格 1 加入适量的胞浆提取缓冲液（请注意样品及裂解液的比例，以达到最佳效果）, 涡旋大力震荡 15 秒, 冰上孵育 5 分钟, 混匀. 接转胞质胞核分离步骤。B. 贴壁细胞1. 贴壁细胞生长至融合度 90
的释放，第二次释放少量O2，第三次O2的释放达到高峰，每4次闪光出现1次放氧峰。用高等植物叶绿体实验得到同样的结果。科克(B.Kok，1970)等人根据这一事实提出了关于H2O裂解放氧的“四量子机理假说”：①PSⅡ的反应中心与H2O之间存在一个正电荷的贮存处(S) ②每次闪光，S交给PSⅡ反应中心1个e-；③当S失去4e-带有4个正电荷时能裂解2个H2O释放1个O2，图中S即为M，按照氧化程度(即带正电荷的多少)从低到高的顺序，将不同状态的M分别称为S0、S1、S2、S3和S4。即S0不带电荷，S1
