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| 识别位点 在不同反应缓冲液中的活性
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文献和实验Determining the Direction of Replication Fork Movement
, with the enzyme of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut
时配制: SED:19mlSE 加1ml 1M DTT PEG/CaT:1:1混合40%PEG及CaT 实验材料 GS115中,用SalI,StuI或SacI线性化的DNA可分离His Mut 重组子 KM71中,用SalI,StuI或SacI线性化的DNA可分离His Muts重组子 亲代载体用相同酶线性化 对照包括无DNA,线性化PBR322DNA及质粒(无细胞)涂板来检测是否有污染
of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut to completion
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