Lectin, Galactoside-Binding, S

Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) 抗体抗原
Lectin, Galactoside-Binding, Soluble, 3 (LGALS3)
别名 MGC52947, MGC75803, LGALS3, leg3, GBP, L-34, Mac-2, gal3, CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, gal-3, CBP 35, Gal-3, Galectin-3, galactoside-binding, galectin-3, lectin, soluble
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (M3-38)
标记
其他选择 非结合性
应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 酵素免疫分析法（EIA）, 流式细胞分析技术（FACS）, 免疫萤光（IF）, Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), 免疫组织化学石蜡技术（IHC-P）
Pubmed 12个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb114277
产品细节 Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) 抗体
免疫原 Plasma membrane glycoproteins from C57BL/6 mouse thioglycollate-elicited peritoneal exudate
克隆位点 M3-38
亚型 IgG2a
交叉反应 （详细） Species reactivity (tested):Mouse.
纯化方法 Protein G Chromatography.
目标详细情况 Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) 抗体
别名 Galectin-3
背景 Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.Synonyms: 35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Lgals3, Mac-2
基因ID 10090
UniProt P16110
研究领域 Neurology
使用细节 Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) 抗体
应用备注 Suitable for use in Indirect Immunofluorescence staining, including Flow Cytometricanalysis of live cells. The addition of Propidium Iodide is optional, its use eliminatesstaining artefacts caused by dead cells (1). This antibody is also suitable for Frozen Sections (4,5), Paraffin Sections (7) and ELISA (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 Flow Cytometry Analysis: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg of antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody FITC Goat anti-rat IgG (H+L) at appropriate dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
限制 仅限研究用
贮存及处理 Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) 抗体
浓度 1.0 mg/mL
缓冲液 PBS with 0.02 % Sodium Azide as preservative
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.