Green Fluorescent Protein (GFP

Green Fluorescent Protein (GFP) (AA 246) 抗体 (HRP)抗原
Green Fluorescent Protein (GFP)
抗原表位
其他选择 AA 246
适用
其他选择 Aequorea victoria
宿主
其他选择 山羊
克隆类型
多克隆
标记
其他选择 HRP
应用范围
其他选择 Western Blotting (WB), 酶联免疫吸附测定（ELISA）, Fluorescence Microscopy (FM)
Pubmed 找到1个引用
规格 1 mg
发货至 中国 (更改)
发货时间 23至28个工作日
产品编号 yb100089
产品细节 Green Fluorescent Protein (GFP) (AA 246) 抗体 (HRP)
免疫原 The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
亚型 IgG
产品特性 Concentration Definition: by UV absorbance at 280 nm
目标详细情况 Green Fluorescent Protein (GFP) (AA 246) 抗体 (HRP)
别名 GFP
背景 HRP Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: HRP, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
研究领域 Tags/Labels
使用细节 Green Fluorescent Protein (GFP) (AA 246) 抗体 (HRP)
应用备注 Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
限制 仅限研究用
贮存及处理 Green Fluorescent Protein (GFP) (AA 246) 抗体 (HRP)
状态 Lyophilized
溶解方式 Restore with deionized water (or equivalent)
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Gentamicin sulfate
注意事项 Do NOT add Sodium Azide!
储存条件 4 °C