L-苹果酸[MegaQuant法]检测试剂盒

Simple colourimetric method for the determination of L-Malic
Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD⁺ ↔ oxaloacetate + NADH + H⁺

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

(diaphorase)
(3) INT + NADH + H⁺ → NAD⁺ + INT-formazan

Kit size: 60 assays
Method: Spectrophotometric at 505 nm
Reaction time: ~ 6 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method