酵母葡聚糖酶检测试剂盒

Colourimetric method for the enzymatic determination of
yeast β-Glucan and also (1-3)-β Glucans

Principle:
(KOH, 4°C, 30 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + H₂O → soluble glucan

(glucazyme, 40°C, 16 h)
(2) Soluble glucan + H₂O → D-glucose

(glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(4) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

Kit size: 50 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 100 min
Detection limit: 1-100% of sample weight
Application examples:
Yeast preparations and other materials
Method recognition: Novel method