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- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Peptidase Inhibitor 16 (PI16)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPQ943Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 71.4kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Leu28~Gly440 (Accession # Q6UXB8) with two N-terminal Tags, His-tag and GST-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its 肽酶抑制因子16(PI16)重组蛋白 sequence is listed below.
MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-LTD EEKRLMVELH NLYRAQVSPT ASDMLHMRWD EELAAFAKAY ARQCVWGHNK ERGRRGENLF AITDEGMDVP LAMEEWHHER EHYNLSAATC SPGQMCGHYT QVVWAKTERI GCGSHFCEKL QGVEETNIEL LVCNYEPPGN VKGKRPYQEG TPCSQCPSGY HCKNSLCEPI GSPEDAQDLP YLVTEAPSFR ATEASDSRKM GTPSSLATGI PAFLVTEVSG SLATKALPAV ETQAPTSLAT KDPPSMATEA PPCVTTEVPS ILAAHSLPSL DEEPVTFPKS THVPIPKSAD KVTDKTKVPS RSPENSLDPK MSLTGARELL PHAQEEAEAE AELPPSSEVL ASVFPAQDKP GELQATLDHT GHTSSKSLPN FPNTSATANA TGGRALALQS SLPGAEGPDK PSVVSGLNSG
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this 肽酶抑制因子16(PI16)重组蛋白 protein is less than 5% within the expiration date under appropriate storage condition.
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文献和实验-Triton蛋白分级抽提试剂盒细胞膜NP-40 or RIPA膜蛋白抽提试剂盒蛋白分级抽提试剂盒细胞核RIPA核蛋白抽提试剂盒线粒体RIPA线粒体蛋白抽提试剂盒亚细胞定位蛋白抽提试剂盒蛋白酶磷酸酶抑制剂抑制剂蛋白酶或磷酸酶PMSF丝氨酸蛋白酶抑制剂,如胰凝乳蛋白酶、胰蛋白酶和凝血酶,也抑制半胱氨酸蛋白酶如木瓜蛋白酶Leupeptin 亮肽素抑制丝氨酸和半胱氨酸蛋白酶,如胰蛋白酶、木瓜蛋白酶、纤溶酶和组织蛋白酶BAprotinin 抑肽酶丝氨酸蛋白酶抑制剂,抑制纤维蛋白溶酶、激肽释放酶、胰蛋白
(Tag)要小得多纯化后蛋白可以直接进行下游操作6×组氨酸可以用于任何表达系统,包括:细菌、杆装病毒和哺乳动物6×组氨酸标签在生理溶液pH下不带电荷标签不会干涉重组蛋白的结构和功能6×组氨酸不会影响蛋白的分泌6×组氨酸标签免疫原性差重组蛋白可以不需要出去标签直接作为抗原进行免疫利用Xa因子蛋白酶可以方便地将6×组氨酸标签有效地切除去除标签的蛋白可以用作晶体成像或者核磁共振研究一些QIAexpress载体带有6×组氨酸二氢叶酸还原酶标签(6×His-DHFR)融合在6×组氨酸DHFT标签中的小肽段在表达
和廉价的[12]。 在启动子下游是RBS,其跨度约为54个核苷酸,两端限定在 -35(±2)和mRNA编码序列的+19到+22之间[13]。Shine-Dalgarno(SD)位点[14,15]在翻译起始阶段与16S rRNA的3’端相互作用[16]。SD与起始密码子间的距离约为5-13bp[17],而且此区的序列在mRNA转录物中应避免出现二级结构,否则将会降低翻译起始的效率[18]。 在RBS的5’和3’端均为A丰富区[19]。转录终止子位于编码序列的下游,作为转录终止的信号
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