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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
Aprotinin from bovine lung
- 库存:
现货
- 供应商:
上海善然生物科技有限公司
- 规格:
1MG
抑肽酶 来源于牛肺
Aprotinin from bovine lung
lyophilized powder, 3-8 TIU/mg solid
Frequently Asked QuestionsLive Chat andFrequently Asked Questions are available for this Product.ApplicationAprotinin is largely used as an inhibitor of trypsin.Preparation NoteThis product is a lyophilized powder with activity of 3-8 TIU/mg of solid powder. Aprotinin is freely soluble in water (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions tend to be less stable than concentrated ones, though solution stability also depends on pH. Aprotinin is relatively stable against denaturation - only thermolysin has been found capable of degrading it at 60-80°C. Sterilizaiton of Aprotinin can be achieved through filtration by a 0.2 μm filter.Unit DefinitionOne Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of Nalpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.Biochem/physiol Actions可以抑制胰蛋白酶、胰凝乳蛋白酶、激肽释放酶以及纤溶酶的丝氨酸蛋白酶抑制剂。
性质
Related CategoriesA to C,Analytical and Industrial Enzymes,Broad Spectrum Inhibitors of Proteolytic Enzyme Classes,Carboxypeptidase Y,Chymotrypsin, α-,
Chymotrypsin, alpha-,Coagulation Proteins and Reagents,Core Bioreagents,D to K,Elastase, leukocyte,Endoproteinase Lys-C,Enzyme Inhibitors,Enzyme Inhibitors by Enzyme,Enzyme Inhibitors by Type,Inhibitors,Kallikrein tissue,Miscellaneous Peptides,P to Q,Peptides and Proteins,Peptides for Cell Biology,Plasma & Blood Proteins,Plasmin,Protease Inhibitor Specificity Index,Protease Inhibitors,Proteins,R to Z,Research Essentials,Serine Protease Inhibitors,Subtilisin,Tissue Plasminogen Activator,Trypsin,Trypsin Inhibitors,生化试剂,细胞信号转导和神经科学,细胞生物学,蛋白质及衍生物,酶、抑制剂和底物更多...form lyophilized powdermol wt mol wt ~6,500solubility H2O: ≥5 mg/mLstorage temp. 2-8°CGene Information cow ... PTI(404172)
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文献和实验磷酰胺(DEPC) (Sigma) 、30% 甘油及蛋白酶抑制剂 [ 含有 1 μg/ml 抑肽酶(aprotinin) 、1 μg/ml 胃蛋白酶抑制剂(pepstatin) 、1 μg/ml 亮抑酶肽(leupeptin) 、0.1 mmol/L PMSF ] ,所有药品都来自于 Sigma ( 见注释 1 和注释 2) 。注意:媒介中的两种成分—— DEPC 和 β-巯基乙醇是有毒的,需要在通风橱中进行准备,并需要戴化学防护手套。 2.2 细胞核的提纯 ( 1 ) 样品
220 Gibco分装 B1092 DNAse Ⅰ,Bovine (脱氧核糖核酸酶) 25mg/100mg 230/430 Sigma分装 B1093 dNTP Mixture,50X (dNTP混合物) 4x 4uM/4x 10uM 160/350
是引起哮喘的因素之一。Kinin作用于皮肤和内脏感觉神经末梢,可引起剧烈疼痛。PGE则能增强和延长其致痛作用。kinin还可促进白细胞的游走和聚集,为重要炎症介质之一。 Kinin通过与靶细胞膜表面的kinin受体B1和B2结合产生作用,其机制可能与激活PLA2,释出AA,产生PGs及对靶组织的直接作用有关。 影响kinin释放酶-kinin系统的药物为抑肽酶(aprotinin)。多来自牛肺,是一种由58个氨基酸组成的kinin释放酶抑制剂,使kinin原不能形成
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