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- sigma
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- A1153-25MG
- 2025年09月28日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 英文名：
Aprotinin from bovine lung
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
- 规格：
1MG
抑肽酶 来源于牛肺
Aprotinin from bovine lung
lyophilized powder, 3-8 TIU/mg solid
Frequently Asked QuestionsLive Chat andFrequently Asked Questions are available for this Product.ApplicationAprotinin is largely used as an inhibitor of trypsin.Preparation NoteThis product is a lyophilized powder with activity of 3-8 TIU/mg of solid powder. Aprotinin is freely soluble in water (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions tend to be less stable than concentrated ones, though solution stability also depends on pH. Aprotinin is relatively stable against denaturation - only thermolysin has been found capable of degrading it at 60-80°C. Sterilizaiton of Aprotinin can be achieved through filtration by a 0.2 μm filter.Unit DefinitionOne Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of Nalpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.Biochem/physiol Actions可以抑制胰蛋白酶、胰凝乳蛋白酶、激肽释放酶以及纤溶酶的丝氨酸蛋白酶抑制剂。
性质
Related CategoriesA to C,Analytical and Industrial Enzymes,Broad Spectrum Inhibitors of Proteolytic Enzyme Classes,Carboxypeptidase Y,Chymotrypsin, α-,
Chymotrypsin, alpha-,Coagulation Proteins and Reagents,Core Bioreagents,D to K,Elastase, leukocyte,Endoproteinase Lys-C,Enzyme Inhibitors,Enzyme Inhibitors by Enzyme,Enzyme Inhibitors by Type,Inhibitors,Kallikrein tissue,Miscellaneous Peptides,P to Q,Peptides and Proteins,Peptides for Cell Biology,Plasma & Blood Proteins,Plasmin,Protease Inhibitor Specificity Index,Protease Inhibitors,Proteins,R to Z,Research Essentials,Serine Protease Inhibitors,Subtilisin,Tissue Plasminogen Activator,Trypsin,Trypsin Inhibitors,生化试剂,细胞信号转导和神经科学,细胞生物学,蛋白质及衍生物,酶、抑制剂和底物更多...form lyophilized powdermol wt mol wt ~6,500solubility H2O: ≥5 mg/mLstorage temp. 2-8°CGene Information cow ... PTI(404172)
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文献和实验磷酰胺（DEPC) (Sigma) 、30% 甘油及蛋白酶抑制剂 [ 含有 1 μg/ml 抑肽酶（aprotinin) 、1 μg/ml 胃蛋白酶抑制剂（pepstatin) 、1 μg/ml 亮抑酶肽（leupeptin) 、0.1 mmol/L PMSF ] ，所有药品都来自于 Sigma ( 见注释 1 和注释 2) 。注意：媒介中的两种成分—— DEPC 和 β-巯基乙醇是有毒的，需要在通风橱中进行准备，并需要戴化学防护手套。 2.2 细胞核的提纯 ( 1 ) 样品
220 Gibco分装 B1092 DNAse Ⅰ,Bovine (脱氧核糖核酸酶) 25mg/100mg 230/430 Sigma分装 B1093 dNTP Mixture,50X (dNTP混合物) 4x 4uM/4x 10uM 160/350
对不对?请指教!Glutathione-agarose(4%cross-linked beaded agarose)和Glutathione Sepharose 4B是一种东西吗? 其次,我想问一下你纯化过GST融合蛋白没有?我现纯化一个融合蛋白(某种酶和GST融合),我买了SIGMA公司的GLUTATHIONE-AGAROSE五毫升,但我不知怎么做.分子克隆说直接将亲和介质于细菌裂解液混允离心....我不知我买的可不可以这样做.而产品说明书上说做柱子,我就想1毫升的柱子怎么做,装介质的柱子的高度
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