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- A1153-25MG
- 2025年09月28日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
低温
- 英文名：
Aprotinin from bovine lung
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
- 规格：
25MG
产品名称
Frequently Asked Questions
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Application
Aprotinin is largely used as an inhibitor of trypsin.
Preparation Note
This product is a lyophilized powder with activity of 3-8 TIU/mg of solid powder. Aprotinin is freely soluble in water (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions tend to be less stable than concentrated ones, though solution stability also depends on pH. Aprotinin is relatively stable against denaturation - only thermolysin has been found capable of degrading it at 60-80°C. Sterilizaiton of Aprotinin can be achieved through filtration by a 0.2 μm filter.
Unit Definition
One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of Nalpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
Biochem/physiol Actions
可以抑制胰蛋白酶、胰凝乳蛋白酶、激肽释放酶以及纤溶酶的丝氨酸蛋白酶抑制剂。
性质
| Related Categories | A to C, Analytical and Industrial Enzymes, Broad Spectrum Inhibitors of Proteolytic Enzyme Classes, Carboxypeptidase Y, Chymotrypsin, α-, |
| form | lyophilized powder |
| mol wt | mol wt ~6,500 |
| solubility | H2O: ≥5 mg/mL |
| storage temp. | 2-8°C |
| Gene Information | cow ... PTI(404172) |
| D6750-100G | 脱氧胆酸钠 |
| 88440-250ML-f | 聚乙二醇200 |
| G1251-500G | L-谷氨酸 |
| C3032-500G | 氯化铯 |
| D1626-1G | DNA(鲱鱼精 ) |
| C3416-5G | 羧苄 |
| I9516-500ml | 异丙醇 |
| A4737-100G | BSA |
| A1112-100G | 海藻酸钠 |
| 56485-250MG | N-羟基琥珀酰亚胺磺酸钠盐 |
| G3126-100G | L-谷氨酰胺 |
| 451614-5G | 碳酸钠 |
| 91077C-1G | 人胰岛素 |
| k1377-5G | 卡那霉素 |
| 215929-500G | 碘酸钾 |
| B9876-500G | 硼砂 |
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文献和实验磷酰胺（DEPC) (Sigma) 、30% 甘油及蛋白酶抑制剂 [ 含有 1 μg/ml 抑肽酶（aprotinin) 、1 μg/ml 胃蛋白酶抑制剂（pepstatin) 、1 μg/ml 亮抑酶肽（leupeptin) 、0.1 mmol/L PMSF ] ，所有药品都来自于 Sigma ( 见注释 1 和注释 2) 。注意：媒介中的两种成分—— DEPC 和 β-巯基乙醇是有毒的，需要在通风橱中进行准备，并需要戴化学防护手套。 2.2 细胞核的提纯 ( 1 ) 样品
220 Gibco分装 B1092 DNAse Ⅰ,Bovine (脱氧核糖核酸酶) 25mg/100mg 230/430 Sigma分装 B1093 dNTP Mixture,50X (dNTP混合物) 4x 4uM/4x 10uM 160/350
用DAPI进行细胞染色DAPI的配置Stock solution of 1mg/ml in ddH2O; working solution of 0.25mg/ml to 50mg/ml in ddH2O or PGM.1.Place sample on slide.2.Add a few drops working solution.3.Squash and view.Staining � DAPI Staining for Cells:Separately DAPI staining
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