CD90 (THY1) 抗体 (PE)

CD90 (THY1) 抗体 (PE)抗原
CD90 (THY1)
别名 CD90, CD7, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2
适用
其他选择 小鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (5a-8)
标记
其他选择 PE
CD90 (THY1) 抗体 (PE)应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 10个引用可参考
规格 0.3 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb114256
产品细节
免疫原 CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
克隆位点 5a-8
亚型 IgG2b
特异性 This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
纯化方法 Protein G Chromatography
目标详细情况
别名 CD90
CD90 (THY1) 抗体 (PE)背景 CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
基因ID 21838
NCBI登录号 NP_033408
UniProt P01831
研究领域 CD Antigens, Surface Receptors of Immune Cells, Experimental Models
使用细节
应用备注 Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: PE-Mouse IgG2b,kCell Source Percentage of cells stained above control: Thymus: 98. 4%Spleen: 28. 8%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR/J
限制 仅限研究用
贮存及处理
浓度 0.1 mg/mL
缓冲液 PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CD90 (THY1) 抗体 (PE)注意事项 This product is photosensitive and should be protected from light
储存条件 4 °C
储存方法 Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!